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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Engineering Shewanella oneidensis enables xylose-fed microbial fuel cell

Fig. 1

Synthetic biology strategies for the construction of four recombinant S. oneidensis strains (namely XE, GE, XS, and GS) to enable xylose utilization and electricity generation of S. oneidensis. Xylose transporter genes included xylT (the gene encoding d-xylose-proton symporter) from Clostridium acetobutylicum and Gxf1 (the gene encoding glucose/xylose facilitator 1) from Candida intermedia. The xylose isomerase pathway included xylA (the gene encoding xylose isomerase) and xylB (the gene encoding xylulokinase) from E. coli. The oxidoreductase pathway included XYL1 (the gene encoding d-xylose reductase), XYL2 (the gene encoding xylitol dehydrogenase), and XKS1 (the gene encoding d-xylulokinase) from Scheffersomyces stipites. Four gene assemblies (plasmids), namely XE, GE, XS, and GS (as shown in the green-dash square) were synthesized for the enhanced xylose transport and metabolism, which transformed into S. oneidensis, respectively, to construct four recombinant S. oneidensis strains

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