Fig. 1

Multiple alignment of amino acid sequences of McCut with homologous cutinases. Numbers on the left are the residue number of the first amino acid in each line. The putative catalytic triad (denoted by stars below the residues) of McCut was predicted by alignment with structure-resolved A. oryzae cutinase (PDB ID: 3GBS_A). Abbreviations and GenBank Accession Numbers or PDB ID of the cutinases in the alignment are as follows: M. cinnamomea S168 (M.c.KY568910.1), A. nidulans FGSC A4 (A.n.Q5AVY9.2), A. oryzae (A.o.3GBS_A), A. niger (A.n.AKE48475.1), H. insolens (H.i.4OYY_A), and Colletotrichum gloeosporioides (C.g.AAL38030.1). Identical residues are shaded in black, and conserved residues are shaded in gray