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Table 2 Substrate specificity of McCut

From: High-level expression and characterization of a novel cutinase from Malbranchea cinnamomea suitable for butyl butyrate production

Substrate

Specific activity (U/mg)

Relative activity (%)

pNP estersa

 pNPA (C2)

553.5 ± 2.5EF

48.2

 pNPB (C4)

1147.9 ± 8.3A

100

 pNPH (C6)

1076.2 ± 5.0B

93.7

 pNPC (C8)

646.9 ± 33.3D

56.3

 pNPD (C10)

607.6 ± 28.3DE

52.9

 pNPL (C12)

494.5 ± 5.0F

43.1

 pNPM (C14)

962.6 ± 5.0C

83.8

 pNPP (C16)

619.5 ± 10.8DE

54.0

Triglyceridesb

 Triacetin (C2)

183.6 ± 4.8B

50.8

 Tributyrin (C4)

361.1 ± 11.6A

100

 Tricaproin (C6)

175.7 ± 9.1B

48.7

 Tricaprylin (C8)

30.7 ± 2.9C

8.5

 Tricaprin (C10)

10.2 ± 0.5D

2.4

 Trilaurin (C12)

7.9 ± 0.4D

2.2

  1. All data are mean values ± standard deviations of triplicate measurements
  2. Mean values associated with different capital letters are siginificantly different within pNP esters or triglycerides
  3. aActivities of McCut towards pNP esters were measured at 45 °C in 50 mM Tris–HCl pH 8.0
  4. bActivities with triglycerides as substrates were performed at 45 °C in 2.5 mM Tris–HCl pH 8.0 containing 0.1% (w/v) Triton X-100 and 0.1% (w/v) arabic gum
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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