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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis

Fig. 2

Transcriptional intergenic links in the cua cluster. a Genetic organization of cua cluster with localization of promoters (arrow) and terminators (stem-loop) predicted by BPROM and FindTerm softwares. b RT-PCR analysis of cua gene cluster. PCR amplification was performed on genomic DNA (upper lane) or cDNA (lower lane) obtained after reverse transcription from a cellobiose grown culture. The primers used to study the transcriptional links between two successive genes were localized at the end of the first gene for the direct primer and at the beginning of the second gene for the reverse primer. Genes are named using their locus-tag number except for the control rpoD. The negative control (C) corresponds to a PCR performed on cDNA using primers targeting an intergenic region between two divergent genes, for which amplification might be detected if genomic DNA is present

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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