- Open Access
A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis
Biotechnology for Biofuels volume 10, Article number: 250 (2017)
Like a number of anaerobic and cellulolytic Gram-positive bacteria, the model microorganism Ruminiclostridium cellulolyticum produces extracellular multi-enzymatic complexes called cellulosomes, which efficiently degrade the crystalline cellulose. Action of the complexes on cellulose releases cellobiose and longer cellodextrins but to date, little is known about the transport and utilization of the produced cellodextrins in the bacterium. A better understanding of the uptake systems and fermentation of sugars derived from cellulose could have a major impact in the field of biofuels production.
We characterized a putative ABC transporter devoted to cellodextrins uptake, and a cellobiose phosphorylase (CbpA) in R. cellulolyticum. The genes encoding the components of the ABC transporter (a binding protein CuaA and two integral membrane proteins) and CbpA are expressed as a polycistronic transcriptional unit induced in the presence of cellobiose. Upstream, another polycistronic transcriptional unit encodes a two-component system (sensor and regulator), and a second binding protein CuaD, and is constitutively expressed. The products might form a three-component system inducing the expression of cuaABC and cbpA since we showed that CuaR is able to recognize the region upstream of cuaA. Biochemical analysis showed that CbpA is a strict cellobiose phosphorylase inactive on longer cellodextrins; CuaA binds to all cellodextrins (G2–G5) tested, whereas CuaD is specific to cellobiose and presents a higher affinity to this sugar. This results are in agreement with their function in transport and signalization, respectively. Characterization of a cuaD mutant, and its derivatives, indicated that the ABC transporter and CbpA are essential for growth on cellobiose and cellulose.
For the first time in a Gram-positive strain, we identified a three-component system and a conjugated ABC transporter/cellobiose phosphorylase system which was shown to be essential for the growth of the model cellulolytic bacterium R. cellulolyticum on cellobiose and cellulose. This efficient and energy-saving system of transport and phosphorolysis appears to be the major cellobiose utilization pathway in R. cellulolyticum, and seems well adapted to cellulolytic life-style strain. It represents a new way to enable engineered strains to utilize cellodextrins for the production of biofuels or chemicals of interest from cellulose.
Cellulose is the most abundant polysaccharide on earth, mostly found in plant-cell wall. This polymer is made up of linear chains of glucosyl residues linked through β-1,4 glycosidic bonds, and arranged in a quasi-crystalline structure that makes it highly recalcitrant to enzymatic hydrolysis. This large reservoir of glucose represents a remarkable potential renewable source of energy to produce biofuels or chemicals as an alternative to fossil-derived products. Conversion of cellulose into biofuel through fermentation process depends on its efficient enzymatic breakdown into fermentable sugars, but also relies on the subsequent uptake and fermentation of the degradation products by fermentative microorganisms.
The model bacterium Ruminiclostridium cellulolyticum is an anaerobic organism able to grow on cellulose as a unique carbon source, as well as on soluble sugars such as xyloglucan, cellobiose and to a lesser extent glucose [1,2,3]. The bacterium performs efficient extracellular degradation of plant cell wall polysaccharides into fermentable sugars thanks to multienzymatic complexes called cellulosomes [4, 5]. This enzymatic system has been extensively studied over the past 20 years, mining the enzymatic diversity, and understanding the cellulosomes’ assembly and functioning in plant cell wall degradation process [4,5,6,7,8,9]. However uptake of the released cellodextrins is crucial for growth on cellulose, and little is known about their transport and utilization into R. cellulolyticum, as well as in other cellulosomes-producing bacteria.
In microbes, sugar uptake is usually mediated by phosphotransferase system (PTS), secondary transporters energized by proton-motive force, or ABC transporter functioning with ATP hydrolysis. In the genome of R. cellulolyticum, no PTS system-encoding genes were found, whereas ABC transporters seem to be largely involved in the uptake of saccharides by this bacterium . ABC-type importers are composed of two transmembrane domains forming the channel of the transporter, two intracellular nucleotide-binding domains (NBD) energizing the system through ATP hydrolysis, and an additional extracellular solute binding protein (SBP) essential to capture and deliver the substrate to the cognate translocator. In R. cellulolyticum, only one ABC transporter was studied to date. It was shown to be specific of 4-glucosyl-backbone xyloglucan dextrins which are subsequently degraded in the cytoplasm by three specific enzymes . In some other Gram-positive cellulolytic bacteria and archaea, some binding proteins of ABC transporters were shown to bind to cellobiose and/or cellodextrins; the role of the cognate ABC-transporters during the growth on cellulose was not established [11,12,13,14,15].
In the cellulolytic bacteria, imported cellodextrins are subsequently degraded by the action of intracellular cellodextrin phosphorylases or cellobiose phosphorylases which belong to the Family-94 of glycosyl hydrolases [16,17,18,19]. These enzymes convert cellodextrins of n glucosyl residues in glucose-1-P and a cellodextrin of n-1 glucosyl residues without investment of ATP. They are therefore assumed to save one ATP molecule when the phosphorylated monosaccharide enters the glycolytic pathway. This energy-saving pathway is of particular importance in anaerobic bacteria in which the yield of ATP is usually low.
Genome analysis of R. cellulolyticum reveals that four genes encode putative phosphorylases located at loci Ccel_1439, 2109, 2354, 3412 . Among them, only the gene located at the locus Ccel_2109 is adjacent to genes encoding a putative ABC transporter and a three-component system forming a cluster of genes. This cluster was named cua for cellulose utilization associated . In the present report, we focused on this gene cluster, and explored its role in cellobiose and cellulose degradation products signalization, uptake, and catabolism.
Sequence analysis of cua gene cluster and its products
The cua gene-cluster from loci Ccel_2109 to Ccel_2115 encompasses seven genes predicted to encode (i) a solute binding protein (CuaD), (ii) a putative two-component system composed of a sensor (CuaS) and a response regulator (CuaR), (iii) a solute binding protein CuaA and two integral membrane proteins CuaB and C, forming a putative ABC transporter, and (iv) a putative GH94 cellobiose phosphorylase named CbpA in the present study (Fig. 1).
Upstream and downstream of the cluster, the genes at loci Ccel_2116 and Ccel_2108 encode a putative chaperon protein and a resolvase, respectively. In the cluster, overlapping genes or short intergenic region are observed between the genes cuaD, cuaR, and cuaS, and between cuaA, cuaB, and cuaC. The largest intergenic region (735 bp) is located between the genes cuaC and cbpA (Fig. 1).
The putative sensor CuaS exhibits features characteristic of bacterial histidine kinase proteins with two predicted transmembrane helices, delimiting a 268 amino acids external loop and a conserved C-terminal cytoplasmic region containing an ATP-binding kinase domain. The putative response regulator CuaR belongs to the AraC/XylS family. Both CuaA and CuaD are predicted to be ABC transporters solute binding proteins (SBP) belonging to Family-1, but they share only 14% sequence identity. This family includes proteins displaying affinity to oligosaccharides, whereas the Family-2 SBPs target monosaccharides . As for SBPs found in other Gram-positive bacteria, an N-terminus typical lipoprotein signal peptidase II is predicted for both proteins. The putative cleavage site is located at amino acids 22 and 20 for CuaA and CuaD, respectively, and the mature proteins are predicted to bear an N-terminal acylated cysteine. CuaB and C are putative transmembrane domains, each of them containing six transmembrane-spanning helices, and the typical signal sequence found in integral membrane proteins of ABC transporters [20, 21].
Analysis of the substrates of CbpA, CuaD, and CuaA
As the cua cluster seems to be implicated in cellobiose/cellulose metabolism , we first investigated the binding abilities of the SBPs and characterized the activity of the putative phosphorylase towards cellodextrins. Three recombinant proteins, rCbpA, rCuaA, and rCuaD, were overproduced in E. coli and purified. Activity and specificity of rCbpA was analyzed on cellobiose and longer cellodextrins by quantifying both the released products including α-d-glucose-1-phosphate, and the consumption of the substrate. The enzyme displays phosphorolytic activity on cellobiose (G2) specifically, with K M and k cat values of 2.85 ± 0.47 mM and 1458.4 ± 108 min−1, respectively, but no activity was detected on longer cellodextrins (G3–G5).
The ability of rCuaD and rCuaA to bind to cellodextrins was analyzed using IsoThermal microCalorimetry (ITC). Both SBPs failed to bind to glucose and arabinose. rCuaA was shown to bind to all tested cellodextrins (G2–G5) (Table 1). In all cases, the titration curve fits with a single binding model with a calculated n (stoichiometry) of 1 (see Additional file 1). Analysis of the K D values for the various cellodextrins in the case of CuaA indicates that the affinity is inversely proportional to the size of the dextrins (G2 > G3 > G4 > G5). On the other hand, rCuaD interacts only with cellobiose but with an affinity 10 times higher for the disaccharide than rCuaA. Although comparable ΔG values were obtained for both proteins (approx. − 9 kcal mol−1), the binding of rCuaA to cellobiose is characterized by a highly favorable enthalpy, whereas rCuaD interacts with cellobiose with both favorable enthalpy and entropy (Table 1), thereby indicating that rCuaA and rCuaD bind to cellobiose with different interaction modes. Overall, these results support the predicted role of these three proteins in the signalization, uptake, and degradation of cellobiose/cellodextrins.
Polycistronic organization and regulation of the expression of the cua genes
Based on their expression patterns determined by qPCR, two groups of genes were identified: the cuaDSR group which is constitutively expressed, and the group encompassing cuaABC and cbpA which is upregulated in the presence of cellobiose or cellulose (see Additional file 2) . To further analyze if they form two distinct polycistronic units, transcriptional intergenic links between the cua genes and their adjacent genes were investigated. Using cDNA prepared from mRNA of a cellobiose grown culture, transcriptional links were observed between the successive genes Ccel_2116-cuaD-cuaS-cuaR and between the genes cuaA-cuaB-cuaC-cbpA, respectively (Fig. 2). In contrast, no amplification was detected between adjacent genes cuaR and cuaA, neither between the gene cbpA and the following gene located at locus Ccel_2108. These results support the presence of two transcriptional units on cellobiose, [Ccel_2116-cuaD-cuaS-cuaR] and [cuaA-cuaB-cuaC-cbpA]. Using bioinformatic tools, terminators were predicted downstream cuaR, and cbpA in agreement with the obtained results (Fig. 2). However, the presence of a promotor and a terminator was also predicted upstream and downstream of the gene at locus Ccel_2116, suggesting that this gene could also be expressed by the way of an independent transcriptional unit. However depending on its strength, the prediction of a terminator downstream of the gene at locus Ccel_2116 does not exclude the possibility of a transcriptional link with the following gene cuaD as it is experimentally observed. A terminator was also predicted at the end of cuaA although a transcriptional link was clearly detected with the following gene cuaB. This terminator might be involved in the regulation of the expression level of cuaA compared to cuaB and C that may lead to an excess of binding protein compared to the transmembrane channel as it was formerly observed for the maltose ABC transporter in E. coli .
Overall the results strongly suggest that the cua genes are organized in two transcriptional units: (Ccel_2116)-cuaD-cuaS-cuaR constitutively expressed, and cuaA-cuaB-cuaC-cbpA which is overexpressed in the presence of cellobiose and cellulose compared to arabinose.
Role of the regulator CuaR
As the expression of some of the genes of the cua cluster is regulated, we tested the ability of CuaR to induce their expression by reconstitution of the induction pathway in E. coli. For that purpose, the gene cuaR was expressed in E. coli under the control of an arabinose inducible promoter in a pBAD24 vector (pBADcuaR). The cells were co-transformed with low copy vector pUA66 derivatives. These vectors carry the gfpmut2 reporter gene encoding a green fluorescent protein (GFP), transcriptionally fused to each of the intergenic sequences located upstream of the genes at loci Ccel_2116 (IG1), Ccel_2115 (cuaD, IG2), Ccel_2112 (cuaA, IG3) or Ccel_2109 (cbpA IG4). A pUA66 vector without any intergenic sequence was used as a negative control (Fig. 3). The fluorescence level measured directly in the cells thus reflects the expression level of the reporter gene. With pBAD24 vector, a basal level of fluorescence was measured for each intergenic region. The fact that this basal level was rather high in the case of the intergenic region IG1 suggests that this sequence was at least partly recognized by an E. coli resident regulator/polymerase. With pBADcuaR and in the presence of arabinose, a drastic increase of the fluorescence was only observed when the reporter gene was fused to IG3 (Fig. 3). This result demonstrates that, in E. coli, CuaR is able to recognize the region upstream of cuaA, and induces the expression of the reporter gene. The gene cuaR therefore probably acts as an activator of the transcription of cuaABC and cbpA in R. cellulolyticum.
Growth of a cuaD mutant strain on cellobiose
To investigate the role of these genes in R. cellulolyticum, the group II intron-based Clostron technology was used to inactivate the genes cuaD and cuaA . The cuaD mutant strain was easily obtained and named MTLcuaD, whereas cuaA mutant could not be obtained in spite of using different targets within cuaA.
On arabinose, MTLcuaD and wild-type strains showed similar growth, but in contrast to the wild-type strain the mutant strain failed to grow on cellobiose, thereby indicating that cuaD is critical for cellobiose utilization (Fig. 4). To analyze further this mutant, the presence of CuaD and CuaA was assayed by western blot analyses using antisera raised against rCuaD and rCuaA, respectively. CuaD and CuaA were both detected in the membrane fraction of the wild-type strain grown on cellobiose or arabinose, but none of these binding proteins were detected in the mutant strain grown on arabinose (Fig. 4b, c). This observation strongly suggests that the inability of the MTLcuaD strain to grow on cellobiose is due to the fact that CuaA and most probably the other components of the ABC transporter are no longer synthesized. As we have shown (see above) that cuaD, cuaS, and cuaR are transcriptionally linked, the insertion of the type II intron in cuaD most probably induced a polar effect on the expression of the genes cuaS and cuaR, as already described for insertional type II intron inactivation in the cip-cel operon of R. cellulolyticum . The expression level of the genes cuaD, cuaS, and R was shown to be about 10 time lower in the mutant strain compared to the wild-type strain both grown on arabinose, confirming the polar effect (see Additional file 3). The disability of the signalization system has severely hampered the expression of the genes cuaABC-cbpA, thus explaining the inability of the MTLcuaD to grow on cellobiose-based medium.
Adaptation of cuaD mutant strain on cellulose
To further analyze its phenotype, the MTLcuaD strain was grown on minimal medium containing crystalline cellulose and growth was followed by measuring the protein content (Fig. 5). The wild-type strain grew up to 150 µg mL−1 from day 1 to day 8, whereas the mutant strain grew very slowly up to day 11–12, after which a growth at the same rate as the wild-type strain was observed. As the enzymatic degradation of the cellulose by the cellulosomes during the culture generates an array of cellodextrins ranging from 2 to 6 glucosyl residues, we analyzed the soluble sugars in the culture supernatant. This analysis revealed an accumulation of cellobiose in the MTLcuaD culture during the first 12 days along with a slight consumption of cellulose (20%), whereas nearly no soluble sugars were detected in the supernatant of the wild-type culture (Fig. 5a, b). After 12 days, the cellobiose disappeared rapidly in the MTLcuaD culture, thereby indicating that the strain was suddenly able to import cellobiose. At the same time, the strain started to consume cellulose faster and to grow with a doubling time similar to the wild-type strain. This adapted mutant strain growing on cellulose was subsequently assayed on cellobiose-based medium and was found to grow as fast as the wild-type strain on the disaccharide (Fig. 6). The mutation introduced by the type II intron is still present in this adapted mutant strain (Fig. 6) and CuaD could not be detected, as in the original MTLcuaD strain (Fig. 4c). Nevertheless, western blot analyses revealed the presence of CuaA although this protein was undetectable in the original mutant strain (Fig. 4b). Altogether, these results strongly suggest that spontaneous mutation(s) occurred allowing the strain to adapt and to use the crystalline polysaccharide thanks to at least the presence of CuaA, suggesting that the growth on cellobiose and cellulose is strongly correlated to the presence of the Cua ABC transporter. The sequencing of the adapted MTLcuaD strain genome indicated that several mutations occurred (see Additional file 4). Among them, the most relevant mutation was a two-base pair deletion within the terminator stem between cuaA and cuaB, reducing the theoretical strength of the terminator by two (Fig. 6). In this context, it is hypothesized that this mutation may lead to a stabilization of the messenger transcribed from cuaABC-cbpA, thereby increasing the production of the Cua ABC transporter and CbpA, and thus the recovery of a normal growth on cellobiose and cellulose.
Importance of the ABC transporter and CbpA for growth on cellobiose and cellulose
To further examine the role of the ABC transporter and of CbpA during the growth on cellobiose and cellulose-based media, we functionally complemented the MTLcuaD mutant strain with replicative vectors carrying either no expression cassette (pSOSzeroTm), the genes cuaABC (pSOScuaABC), the genes cuaABC-cbpA (pSOScuaABC-cbpA), or the gene cbpA alone (pSOS956cbpA). All the genes were cloned under the control of a constitutive promoter, but the native gene organization found in R. cellulolyticum was maintained except for the vector pSOScuaABC-cbpA where the large intergenic region (735 bp) naturally occurring between cuaC and cbpA was shortened to 30 bp, including a ribosome binding site to secure the expression of cbpA into the operonic structure.
Western blot analyses confirmed the presence of CuaA in the mutant strains transformed with either pSOScuaABC or pSOScuaABC-cbpA (Fig. 7a). On arabinose, the growth of the transformed R. cellulolyticum mutant strains was not affected by the presence of the vectors compared to the wild-type strain (see Additional file 5). As expected on cellobiose, the mutant strain containing the control vector failed to grow, but complementation with the vector pSOScuaABC-cbpA restored the growth although with a longer generation time (18 h) compared to the wild-type strain. The growth of the mutant strain complemented with the vector carrying only the genes encoding the ABC transporter (pSOScuaABC) could not exceed a final OD value of 0.2 thus showing that the presence of the ABC transporter without CbpA cannot restore the wild-type phenotype. In this strain, cellobiose might be imported but is not optimally catabolized in the absence of CbpA thus leading to a very limited growth on the disaccharide. These results establish that CbpA plays a critical role during the growth on cellobiose. Complementation with the vector pSOScbpA failed to restore any growth on cellobiose, thereby indicating that the cells cannot import cellobiose in the absence of the Cua ABC transporter. Altogether, these results indicate that in R. cellulolyticum, the ABC transporter and CbpA, whose gene expression is induced by the three-component system, are essential for both uptake and utilization of cellobiose. Nevertheless, it cannot be ruled out that the signalization mediated by cuaDSR may regulate the expression of other genes involved in cellobiose metabolism.
The degradation of cellulose performed by the cellulosomes releases cellobiose and longer cellodextrins. On this substrate, the mutant strain carrying the control vector behaved exactly like the untransformed mutant strain: after a slow growth of 12 days during which cellobiose accumulation was shown in the culture supernatant, the MTLcuaD (pSOSzeroTm) adapts and starts to consume rapidly cellobiose, to grow fast, and to produce CuaA (Figs. 4b, 7). This result highlights the importance of Cua ABC transporter during the growth on cellulose. As it was observed on cellobiose, the growth of the mutant strain carrying the vector pSOScuaABC-cbpA is restored on cellulose although the growth is slightly delayed compared to the wild-type strain. As for the wild-type strain, cellobiose did not accumulate in the supernatant during the culture on cellulose of the MTLcuaD(pSOScuaABC-cbpA) strain (see Additional file 6). The functional complementation of the mutant strain with only the ABC transporter also restored the growth on cellulose, although the final protein content at the stationary phase reached a lower value compared to wild-type and mutant MTLcuaD (pSOScuaABC-cbpA) strains (Fig. 7). This result markedly differed from the data obtained for the same strain on cellobiose (nearly no growth). It could be explained by the fact that the ABC transporter can also import longer cellodextrins since CuaA was shown to bind to G2–G5. In this case, the imported longer cellodextrins may support the observed growth of the MTLcuaD (pSOScuaABC) strain on cellulose, though the action of other intracellular enzyme(s) than CbpA, like dextrins hydrolases or phosphorylases.
On cellulose, the MTLcuaD (pSOScbpA) strain started to grow 5 days later than the mutant strain, suggesting that an adaptation also occurred in this strain. The extended slow growth phase observed is probably attributable to the overproduction of CbpA in MTLcuaD (pSOScbpA) strain that may reduce the fitness of the strain during this initial growth phase on cellulose.
Similar combined signalization and transport systems are described in other bacteria [25,26,27,28]. In Gram-negative bacteria, such genes clusters encode only one SBP that is implicated in both signalization and transport purposes, whereas in Gram-positive bacteria two SBPs are encoded like in R. cellulolyticum. In C. beijerinckii, each SBP was shown to be specifically dedicated for either signalization or transport . In R. cellulolyticum, the studied SBPs also appear to have different functions: CuaA operates with the ABC transporter channel and helps to collect a large set of cellodextrins with a moderate affinity. In contrast, CuaD is probably committed to signalization since it was shown to bind specifically to cellobiose with a very high affinity and is genetically associated with the two-component system, thus forming the third subunit of a three-component system. CuaD would therefore sense the presence of cellobiose which is the shortest and most abundant degradation product released from cellulose by the cellulosomes in vitro .
Altogether, the data reported in the present study enable to propose the model depicted in Fig. 8. During the growth on cellulose, the cellulosomes secreted by R. cellulolyticum release a mix of cellodextrins from the substrate, among which cellobiose is detected by the solute binding protein CuaD of the three-component system CuaDSR. This signalization system is believed to transduce this signal via the sensor CuaS to the regulator CuaR which in turn would activate the transcription of the genes cuaABC and cbpA encoding the ABC transporter and the cellobiose phosphorylase CbpA, respectively. The ABC-transporter would then import the various released cellodextrins. The cua gene cluster does not encode any ABC transporter ATPase but the corresponding gene could be located elsewhere on the chromosome as formerly observed for other Gram-positive bacteria [29,30,31,32]. For instance, in Bacillus subtilis, the gene encoding the ABC ATPase MsmX was reported to be encoded in the genome by an isolated gene, and the corresponding protein was found to energize several ABC transporters dedicated to oligosaccharides uptake . In this respect, an orthologous gene to msmX was identified in the genome of R. cellulolyticum at locus Ccel_2909. The gene product shares 65% homology with MsmX, and could energize the cellobiose/cellodextrin ABC transporter. This NBD could also energize other ABC transporters like the xyloglucan dextrins ABC transporter recently described, for which no ABC ATPase-encoding gene was found in the cluster encoding the system . After uptake, CbpA would convert specifically cellobiose into glucose and glucose-1-P, as it was reported for other reported cellobiose phosphorylases [33, 34], whereas the degradation of larger imported cellodextrins (G3–G5) would require other enzyme(s). Four genes encoding putative cytoplasmic GH94 cellodextrin phosphorylases (at loci Ccel_1439, Ccel_2354, Ccel_3412), or β-glucosidases GH1 (Ccel_1139 and Ccel_0374) could be involved in this process [4, 35].
In cellulolytic bacteria such as C. thermocellum or Ruminococcus albus, phosphorolytic activity was however reported to dominate hydrolytic activity [16, 18]. As the phosphorolytic cleavage mechanism saves ATP during sugar catabolism, such process ought to be especially important for obligate anaerobic cellulolytic bacteria which grow with low ATP yields and need in addition to synthesize and secrete numerous cellulases to achieve an efficient degradation of the cellulose. From an energetic point of view, ABC transporter systems are assumed to require more energy to import carbohydrate compared to PTS uptake systems, which are not found in R. cellulolyticum . Nevertheless, the higher cost in ATP to transport cellodextrins through ABC transporters may be balanced by the import of long cellodextrins which advantageously deliver more glucose units than short cellodextrins for the same cost, as it occurs in C. thermocellum . Another advantage of the ABC transporters is that they display high uptake efficiency at low substrate concentration in the medium, thanks to the presence of the solute binding protein . In R. cellulolyticum, the capture and import of cellodextrins is indeed rather efficient since nearly no soluble sugars are detected in the culture supernatant of the wild-type strain during the growth on cellulose. This efficient transport system might therefore contribute to increase the efficiency of the cellulolysis by preventing feedback inhibition of the cellulases by cellodextrins. In addition, the efficiency of the ABC transporter may help to reach the concentration threshold of intracellular cellobiose needed to thermodynamically favor phosphorolysis by CbpA .
Overall, the CuaABC transporter seems well adapted to anaerobic degradation of cellulose in R. cellulolyticum by scavenging efficiently cellodextrins present at low concentrations during the degradation of cellulose, reducing feed-back inhibition of the cellulases, and ensuring a requisite flow of substrate into the cell able to support phosphorolysis reaction. It is therefore not surprising that similar gene clusters are found in other anaerobic and cellulolytic bacteria whose genome was sequenced (Fig. 9). Recently, to produce cellulosic biofuel, synthetic biology strategies aimed to make yeasts and E. coli strains able to use cellobiose rather than glucose . This was done through the acquisition of intracellular β-glucosidase, cellobiose phosphorylase, and/or cellobiose transporters from heterologous organisms [37,38,39,40], but none of the selected transporters used were ABC transporters. The efficient and energy-saving system of the conjugated transport/phosphorolysis described in the present study was shown to be well adapted to the low concentrations of soluble sugars released during cellulolysis, and to provide high enough intracellular sugar concentration for efficient phosphorolysis. This system adapted to cellulolytic life-style could therefore represent an attractive way to engineer strains for biofuels or chemicals production from ligno-cellulosic substrates.
The present study identified a three-component signalization system inducing the expression of genes encoding an ABC transporter and a cellobiose phosphorylase. Through the study of a mutant strain and its derivatives, the genes encoding the ABC transporter and the cellobiose phosphorylase were shown to be essential for the growth of R. cellulolyticum on cellobiose and cellulose. This uptake and intracellular degradation of cellobiose system appears to be the major cellobiose utilization pathway in R. cellulolyticum. It seems well adapted to cellulolytic life-style and could be used to engineer new strain to produce biofuels and chemicals of interest from cellodextrins.
Strains, media and vectors
Strains used in this study are reported in Additional file 7. Escherichia coli strains were grown at 37 °C in Luria–Bertani medium supplemented with appropriate antibiotic (100 µg mL−1 of ampicillin or 35 µg mL−1 chloramphenicol). R. cellulolyticum H10 ATCC 35319  and mutants were grown anaerobically at 32 °C on basal medium  or minimal medium (basal medium without yeast extract), supplemented with either 2 g L−1 cellobiose, arabinose, or 5 g L−1 Sigmacell20 (Sigma-Aldrich, Saint Louis, MO). When necessary, thiamphenicol (5 µg mL−1) or erythromycin (2.5 µg mL−1) was added to the medium. Colonies of recombinant R. cellulolyticum mutant strains were isolated under the anaerobic atmosphere of a glove box as described previously . Growth on soluble substrate was followed by monitoring OD at 450 nm over time (arabinose, cellobiose). When cultured on 5 g L−1 Sigmacell, growth measurements were based on protein content quantification as described previously .
Sequencing of strains was performed from genomic DNA by GATC (Konstanz, Germany).
Analysis of cellulose- and soluble cellodextrin-content during growth
During growth, samples of culture were harvested and centrifuged. The cellulose pellets and the soluble sugars present in the supernatants were analyzed using high-pressure anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD). After centrifugation, the pellets of the different growth samples were resuspended in 500 μL of 12 M H2SO4 and incubated for 1 h at 37 °C under shaking. For each sample, 20 µL was diluted into 220 μL of distilled water and autoclaved for 1 h at 120 °C. After cooling down, 50 μL of 10 M NaOH was added and the sample was centrifuged at 10,000g for 10 min at room temperature. 10 µL of supernatant was mixed with 190 μL of distilled water and 50 μL of 0.5 M NaOH. Glucose was quantified by high-pressure anion exchange chromatography using a Dionex ICS 3000 (Sunnyvale, CA) equipped with a pulsed amperometric detector (HPAEC-PAD). 25 µL was applied to a Dionex CarboPac PA1 column (4 × 250 mm) preceded by the corresponding guard column (4 × 50 mm). Sugars were eluted with the buffers 0.1 M NaOH and 0.5 M sodium acetate + 0.1 M NaOH as the eluants A and B, respectively, using for glucose the multi-step procedure: isocratic separation (5 min, 95% A + 5% B), column wash (2 min, 99% B), and subsequent column equilibration (2.5 min, 95% A + 5% B). Injection of samples containing glucose (Sigma) at known concentrations (5–100 nM) was used to quantify the glucose content.
The culture supernatants were analyzed similarly except that 20 µL of supernatant was mixed with 190 μL of distilled water and 50 μL of 0.5 M NaOH, prior HPAEC-PAD analyses. In that case, sugars were eluted using the multi-step procedure: isocratic separation (5 min, 95% A + 5% B), separation gradient (8 min, 10 to 37% B), column wash (2 min, 99% B), and subsequent column equilibration (2.5 min, 95% A + 5% B). Injections of samples containing glucose, cellobiose (Sigma), cellotriose, cellotetraose, and cellopentaose (Megazyme) at known concentrations (5–100 nM) were used to identify and quantify the cellodextrins present in the culture supernatants.
Construction of cuaD mutations in R. cellulolyticum
Gene inactivation in R. cellulolyticum was performed using the ClosTron technology as described by Heap et al.  with minor modifications to create the pMTL007cuaD and pMTL007cuaA [23, 24]. The integration sites in the target genes and the primers used to retarget the Ll.LtrB intron in the pMTL007 [IBS, EBS1d and EBS2] (see Additional file 8) were generated by the Perutka algorithm (http://ClosTron.com). Specific cuaD and cuaA target primers were used to produce a fragment by overlapping PCR using pMTL007 as the matrix. The fragments and the recipient pMTL007 were subsequently digested by BsrGI and HindIII and ligated, creating the pMTL007cuaD and pMTL007cuaA to be used for electro-transformation of R. cellulolyticum. Electro-transformation was performed as previously described [43, 44]. The modified strain interrupted in the gene at locus Ccel_2115 was called MTLcuaD.
Complementation of MTLcuaD mutant
As the MTLcuaD-mutated strain already contains erythromycin resistance brought by the insertion in the genome, we used a thiamphenicol-resistant vector pSOSzeroTm . This vector was digested using SalI and ligated with the expression cassette obtained from pSOS954 digested by the same enzyme. The resulting E. coli–R. cellulolyticum shuttle expression vector called pSOS956 was then used to clone the genes cuaABC, cuaABC-cbpA, and cbpA. Amplicons were obtained by PCR using genomic DNA of R. cellulolyticum as the template and the primers pairs 2112BamDir/2110NarRev, and 2109BamDir/2109NarRev. Corresponding amplicons were digested with BamHI and NarI, and ligated to pSOS956 similarly digested to give the vectors pSOScuaABC and pSOScbpA. To construct the vector pSOScuaABC-cbpA, the PCR product obtained using the primers pair 2109NarDir/2109NarRev on genomic DNA from R. cellulolyticum was digested with NarI and ligated in the NarI-digested pSOScuaABC. This construction brings cbpA downstream of cuaABC under the control of the same promotor, but it removes the intergenic region found naturally upstream of cbpA. The resulting vectors were called pSOSzeroTm, pSOSABC, pSOSABC-cbpA, and pSOScbpA, and were transferred in the MTLcuaD mutant strain as previously described [43, 44].
RNA preparation and reverse transcription
Ruminiclostridum cellulolyticum was grown in minimal medium supplemented with arabinose (2 g L−1), cellobiose (2 g L−1), or cellulose (5 g L−1). Samples were harvested at mid or late-exponential phase of growth (8000 g 10 min) and frost at − 80 °C. Total RNAs were isolated from the pellet using the Maxwell® 16 LEV simplyRNA Blood Kit (Promega) according to the manufacturer’s instructions and an extra TURBO DNase digestion step to eliminate the contaminating DNA. The RNA quality was assessed by an Experion chip (Bio-Rad), and the absence of DNA contamination was confirmed by PCR. RNA was quantified spectrophotometrically at 260 nm (NanoDrop 1000; Thermo Fisher Scientific). For cDNA synthesis, 1 µg total RNA and 0.5 μg random primers (Promega) were used with the GoScript™ Reverse transcriptase (Promega) according to the manufacturer instruction.
Quantitative real-time-PCR for transcriptional analyses
Intergenic links and qPCR analysis were performed on cDNA using primers listed in Additional file 8. Intergenic links were analyzed as previously described . qPCR was carried out on a CFX96 real-time PCR detection system (Biorad, France) and the software Manager CFX 3.1 using RNA16S gene as a reference for normalization. For qPCR, the reaction volume was 15 μL and the final concentration of each primer was 0.5 μM. The cycling parameters of the qPCR were 98 °C for 2 min, followed by 45 cycles of 98 °C for 5 s, 56 °C for 10 s, 72 °C for 1 s. A final melting curve from 65 to 95 °C is added to determine the specificity of the amplification. To determine the amplification kinetics of each product, the fluorescence derived from the incorporation of EvaGreen into the double-stranded PCR products was measured at the end of each cycle using the SsoFast EvaGreen Supermix 2X Kit (Bio-Rad, France). The results were analyzed using Bio-Rad CFX Manager software, version 3.0 (Bio-Rad, France). For each point, a technical duplicate was performed. The amplification efficiencies for each primer pairs comprised between 80 and 100%.
The gene cuaR was amplified by PCR using the forward and the reverse primers pBadRegFLEcoD and pBadRegPstR, and the amplicon was digested by EcoRI and PstI before ligation with EcoRI-PstI linearized pBAD24  to give pBAD-cuaR. Intergenic regions (IG) located upstream of the genes at the locus Ccel_2116, cuaD, cuaA, and cbpA were amplified using forward and reverse primers pairs IG1gfpXhoD/IG1gfpBamR, IG2gfpXhoD/IG2gfpBamR, IG3gfpXhoD/IG3gfpBamR, IG4gfpXhoD/IG4gfpBamR respectively. Amplicons were digested using XhoI and BamHI, and ligated to linearized pUA66 vector allowing transcriptional fusions with gfpmut2 gene (see Additional file 8) . The vectors pBAD24, pBAD24cuaR, and pUA66, pUA66-IG1, pUA66-IG2, pUA66-IG3, and pUA66-IG4 were transferred in MG1665 E. coli strain for fluorescence measurements. Recombinant MG1655 E. coli cells containing a pBAD24 and a pUA66 derivative vector were grown overnight in minimal medium M9, 0.2% casamino acids (Gifco, USA), with 100 µg mL−1 ampicillin and 25 µg mL−1 kanamycin. Cultures were diluted in the same medium at an OD of 0.1 in a 96-well plate (Greiner, Germany). Either 0.1% arabinose or the same volume of water was added, and the plate was incubated at 37 °C overnight under gentle shaking. OD at 600 nm and GFP fluorescence (488 nm and 521 nm excitation and emission wavelengths, respectively) were read using a Tecan i-control™ 1.9.17.
Cloning of the genes encoding rCuaA and rCuaD and rCbpA in E. coli
Recombinant mature proteins rCuaA, rCuaD, and rCbpA were designed to contain 6 histidines residues at their N-terminus (rCuaA, rCuaD) or at the C-terminus (rCbpA). All genes were amplified by PCR using the genomic DNA of R. cellulolyticum and the forward and reverse primers pairs CuaANdeID/CuaAXhoIR, CuaDNdeID/CuaDXhoIR, CbpANdeID/CbpAXhoIR for the production of rCuaA, rCuaD, and rCbpA, respectively. The amplicons were digested with NdeI and XhoI and cloned in a NdeI–XhoI linearized pET22b(+), thereby generating the pET-cuaA pET-cuaD, or digested with NcoI and XhoI and cloned into a NcoI-XhoI linearized pET28a(+) generating pET-cbpA. The generated plasmids were used to transform BL21(DE3) strain to produce the corresponding recombinant proteins.
Production and purification of the recombinant proteins
Recombinant E. coli BL21(DE3) containing the vectors pETcuaA, pETcuaD, or pETcbpA were grown at 37 °C under shaking to an optical density at 600 nm of 1.0, IPTG was added to a final concentration of 200 µM, and the cultures were incubated overnight under shaking at 16 °C. The cells were then harvested by centrifugation for 15 min at 5000g and broken in a French press before to proceed to a purification through affinity chromatography and anion exchange chromatography as described before . The purified protein concentration was determined by measuring the absorbance at 280 nm.
PAGE and Western blot analysis
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed as previously described . Primary antibodies were obtained using the purified recombinant rCuaA and rCuaD proteins (Eurogentec, France).
Preparation of membrane proteins from R. cellulolyticum
Ruminiclostridum cellulolyticum cells were harvested from a minimal medium grown culture (90 mL) at mid exponential stage of growth (7000g, 15 min, 4 °C). Pelleted cells were broken in buffer containing 50 mM KH2PO4, 150 mM NaCl, pH7, using a French press. After centrifugation (45,000g, 30 min), the pellets containing the membranes were collected in the same buffer containing 1% SDS. Protein content was determined using the Lowry method . Membrane proteins (5 µg) were loaded on a SDS-PAGE to check the quality of the samples (Additional file 9).
Isothermal titration calorimetry (ITC)
Ligand binding parameters were measured using a Microcal iTC200 calorimeter (Malvern) at 20 °C in 150 mM, NaCl, 25 mM HEPES, pH 7.0. Concentrated solutions of glucose, arabinose, cellobiose (Sigma, St Louis MO) and cellotriose, cellotetraose, cellopentaose (Megazyme, Ireland) were diluted to a concentration varying between 100 and 600 µM. The protein concentration in the cell (200 µL) was diluted in the same buffer to a concentration varying between 10 and 60 µM. Data were fitted by nonlinear regression using a single-site model in the Origin 7 software package.
Phosphorylase activity measurement
rCbpA (0.5 µM) was incubated with 0.05–9 mM cellobiose (Sigma) in 25 mM phosphate buffer pH 6 containing 0.01% (w/v) NaN3 in a final volume of 200 µL at 37 °C during 2 min. Then 50 µL of 0.5 M sodium hydroxide was added prior to analysis by HPAEC-PAD, using the same procedure as for cellodextrin content analyses, except that various concentrations of glucose, cellobiose, and α-d-glucose-l-phosphate (Sigma) were used to identify and quantify the released sugars. Activity of rCbpA on 1 mM cellotriose, cellotetraose, and cellopentaose was assayed with 1 µM of enzyme and incubation times up to 120 min at 37 °C were used.
Nucleotide sequences analysis were performed using BPROM, FindTerm (http://www.softberry.com) or ARNold (http://rna.igmors.u-psud.fr). Amino acid sequence analysis were performed using on line tools: Signal P, LipoP (http://www.cbs.dtu.dk), BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and ClustalW2 (http://www.ebi.ac.uk).
solute binding protein
nucleotide binding domain
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SP designed the research. AF, MM, NF, NL, YD, HPF performed the experiments. SP, AF, MM, HPF, YD, RB, PDP analyzed the data. SP and HPF wrote the manuscript. All authors read and approved the final manuscript.
The authors acknowledge Professors Nigel N. Minton and John T. Heap (University of Nottingham, UK) as creators of the transferred material pMTL007. We thank Chantal Tardif, Sandrine Pages, Mireille Ansaldi and Cécile Jourlin for fruitful discussions.
The authors declare that they have no competing interests.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This research was supported by a fellowship from the Ministère de l’Enseignement Supérieur et de la Recherche to AF, and from Erasmus+ Agency to NL, and by a grant from the Agence Nationale de la Recherche (Grant Number: ANR-14-CE05-0019-01).
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Additional file 3. Comparison of cua genes expression level between WT and MTLcuaD mutant strain grown on arabinose. qPCR was performed on cDNA prepared on total RNA extracted from cultures of R. cellulolyticum WT and MTLcuaD strains grown on minimal medium containing 2 g.L−1 arabinose. Expression of each gene of the mutant strain MTLcuaD is presented compared to the WT gene expression normalized to 1.
Additional file 4. Mutations found in the sequence of the adapted MTLcuaD mutant strain. The table reports all single-nucleotide polymorphism (SNP) and insertions/deletions mutations (In/Del) type which were common to two individual MTLcuaD mutant strains clones and absent from the MTLcuaD mutant strain.(white background), and the mutations that were found only in the mutant strain (grey background).
Additional file 6. Growth of two complemented MTLcuaD strains on cellulose. The data shows the growth, the degradation of cellulose and the concentration of cellobiose in the supernatant of the culture of the strains MTLcuaD (pSOScuaABC) and MTLcuaD (pSOScuaABC-cbpA) on minimal medium containing 5 g.L−1 cellulose.
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Fosses, A., Maté, M., Franche, N. et al. A seven-gene cluster in Ruminiclostridium cellulolyticum is essential for signalization, uptake and catabolism of the degradation products of cellulose hydrolysis. Biotechnol Biofuels 10, 250 (2017). https://doi.org/10.1186/s13068-017-0933-7
- Three-component system
- Cellobiose phosphorylase