Table 2 Plasmids and strains used in this study
Strains or plasmids | Description | Source |
|---|---|---|
E. coli XL1-blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIq Z∆M15 Tn10 (Tetr)] | Lab stock |
P. pastoris strains | ||
 X33 | Wilde type | Lab stock |
 X33-SD | P. pastoris X33 harbouring codon-optimized AlsS and AlsD gene | This study |
 X33-SDB | P. pastoris X33 harbouring codon-optimized AlsS and AlsD and S. cerevisiae BDH1 gene | This study |
 X33-SDU | P. pastoris X33 harbouring codon-optimized AlsS and AlsD and E. coli udhA gene | This study |
Plasmids | ||
 pGAPZαA | GAP promoter, α-signal, Zeocin resistance | Invitrogen |
 pGAPZ | GAP promoter, Zeocin resistance | This study |
 pGAPZ-AlsS | pGAPZ harbouring AlsS gene | This study |
 pGAPZ-AlsD | pGAPZ harbouring AlsD gene | This study |
 pGAPZ-BDH1 | pGAPZ harbouring BDH1 gene | This study |
 pGAPZ-udhA | pGAPZ harbouring udhA gene | This study |
 pGAPZ-SD | pGAPZ harbouring AlsS and AlsD genes | This study |
 pGAPZ-SDB | pGAPZ harbouring AlsS, AlsD and BDH1 genes | This study |
 pGAPZ-SDU | pGAPZ harbouring AlsS, AlsD and udhA genes | This study |
 pGAPZ-SDH | pGAPZ harbouring AlsS, AlsD and HIS4 genes | This study |
 pGAPZ-SDBH | pGAPZ harbouring AlsS, AlsD, BDH1 and HIS4 genes | This study |
 pGAPZ-SDUH | pGAPZ harbouring AlsS, AlsD, udhA and HIS4 genes | This study |