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Table 2 Plasmids and strains used in this study

From: Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

Strains or plasmids Description Source
E. coli XL1-blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIq Z∆M15 Tn10 (Tetr)] Lab stock
P. pastoris strains
 X33 Wilde type Lab stock
 X33-SD P. pastoris X33 harbouring codon-optimized AlsS and AlsD gene This study
 X33-SDB P. pastoris X33 harbouring codon-optimized AlsS and AlsD and S. cerevisiae BDH1 gene This study
 X33-SDU P. pastoris X33 harbouring codon-optimized AlsS and AlsD and E. coli udhA gene This study
Plasmids
 pGAPZαA GAP promoter, α-signal, Zeocin resistance Invitrogen
 pGAPZ GAP promoter, Zeocin resistance This study
 pGAPZ-AlsS pGAPZ harbouring AlsS gene This study
 pGAPZ-AlsD pGAPZ harbouring AlsD gene This study
 pGAPZ-BDH1 pGAPZ harbouring BDH1 gene This study
 pGAPZ-udhA pGAPZ harbouring udhA gene This study
 pGAPZ-SD pGAPZ harbouring AlsS and AlsD genes This study
 pGAPZ-SDB pGAPZ harbouring AlsS, AlsD and BDH1 genes This study
 pGAPZ-SDU pGAPZ harbouring AlsS, AlsD and udhA genes This study
 pGAPZ-SDH pGAPZ harbouring AlsS, AlsD and HIS4 genes This study
 pGAPZ-SDBH pGAPZ harbouring AlsS, AlsD, BDH1 and HIS4 genes This study
 pGAPZ-SDUH pGAPZ harbouring AlsS, AlsD, udhA and HIS4 genes This study