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Table 4 Effect of cultivation conditions

From: Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

  Agitation (rpm) Aeration (vvm) pH
300 400 500 0.1 0.5 1.0 4 5 6 Uncontrolled
OD600 23.65 ± 1.5α 34.8 ± 1.9β 42.35 ± 2.61γ 17.6 ± 0.8δ 22.95 ± 0.85α 25.1 ± 1α 17 ± 0.9δ 22.95 ± 1α 23 ± 1.2α 23.85 ± 1.3α
2,3-BD (g/L) 8.34 ± 0.62α 4.36 ± 32β ND 11.07 ± 0.5γ 10.78 ± 0.3γ 8.87 ± 0.2α 10.82 ± 0.2γ 10.78 ± 0.6γ 10.97 ± 0.7γ 10.77 ± 0.9γ
Acetoin (g/L) 1.17 ± 0.02α 1.37 ± 0.02β 9.27 ± 0.81γ 0.97 ± 0.08δ 1.6 ± 0.12ε 1.89 ± 0.15ε 0.91 ± 0.04α 1.63 ± 0.1γ 2.75 ± 0.3ζ 0.99 ± 0.05α
Glycerol (g/L) 3.49 ± 0.1α ND ND 4.95 ± 0.3β 3.45 ± 0.2α 2.26 ± 0.2γ 4 ± 0.2δ 3.46 ± 0.18α 0.46 ± 0.03ε 4 ± 0.3δ
Ethanol (g/L) 0.66 ± 0.01α ND ND 2.43 ± 0.2β 0.46 ± 0.1α ND 0.2 ± 0.01γ 0.47 ± 0.05α 0.01 ± 0δ 0.2 ± 0.01γ
  1. Data indicate average values of duplicate experiments with standard deviation. Values with different Greek symbols between columns were significantly different (P < 0.05). ND indicates not detected