Biotechnology for Biofuels and Bioproducts

Table 4 Effect of cultivation conditions

From: Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

	Agitation (rpm)			Aeration (vvm)			pH
	300	400	500	0.1	0.5	1.0	4	5	6	Uncontrolled
OD₆₀₀	23.65 ± 1.5^α	34.8 ± 1.9^β	42.35 ± 2.61^γ	17.6 ± 0.8^δ	22.95 ± 0.85^α	25.1 ± 1^α	17 ± 0.9^δ	22.95 ± 1^α	23 ± 1.2^α	23.85 ± 1.3^α
2,3-BD (g/L)	8.34 ± 0.62^α	4.36 ± 32^β	ND	11.07 ± 0.5^γ	10.78 ± 0.3^γ	8.87 ± 0.2^α	10.82 ± 0.2^γ	10.78 ± 0.6^γ	10.97 ± 0.7^γ	10.77 ± 0.9^γ
Acetoin (g/L)	1.17 ± 0.02^α	1.37 ± 0.02^β	9.27 ± 0.81^γ	0.97 ± 0.08^δ	1.6 ± 0.12^ε	1.89 ± 0.15^ε	0.91 ± 0.04^α	1.63 ± 0.1^γ	2.75 ± 0.3^ζ	0.99 ± 0.05^α
Glycerol (g/L)	3.49 ± 0.1^α	ND	ND	4.95 ± 0.3^β	3.45 ± 0.2^α	2.26 ± 0.2^γ	4 ± 0.2^δ	3.46 ± 0.18^α	0.46 ± 0.03^ε	4 ± 0.3^δ
Ethanol (g/L)	0.66 ± 0.01^α	ND	ND	2.43 ± 0.2^β	0.46 ± 0.1^α	ND	0.2 ± 0.01^γ	0.47 ± 0.05^α	0.01 ± 0^δ	0.2 ± 0.01^γ

Data indicate average values of duplicate experiments with standard deviation. Values with different Greek symbols between columns were significantly different (P < 0.05). ND indicates not detected

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