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Table 2 The substrate specificities of GtCel5 and its variants

From: Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6

Enzymes CMC-Na (U/mg) Barley β-glucan (U/mg)
GtCel5 1117 ± 43 6257 ± 26
N233G 1901 ± 21 8383 ± 57
N233A 1419 ± 22 7866 ± 56
N233D 1185 ± 35 5869 ± 33
N233S 1039 ± 26 5474 ± 43