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Table 2 The substrate specificities of GtCel5 and its variants

From: Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6

Enzymes

CMC-Na (U/mg)

Barley β-glucan (U/mg)

GtCel5

1117 ± 43

6257 ± 26

N233G

1901 ± 21

8383 ± 57

N233A

1419 ± 22

7866 ± 56

N233D

1185 ± 35

5869 ± 33

N233S

1039 ± 26

5474 ± 43

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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