Fig. 3

RNA blotting of the wild-type and transplastomic C. reinhardtii strains containing the cel6A genes regulated by the 16S promoter and atpA 5′UTR chimeric construct. The RNA bands from the ethidium-bromide-stained agarose gel are shown in the top gel. Total RNA was hybridized with a radiolabeled cel6A probe to evaluate the accumulation of cel6A transcripts in cel6A, NPTII-cel6A, and TetC-cel6A transplastomic strains as well as its absence in the wild-type RNA sample (bottom gel)