Iwai M, Ikeda K, Shimojima M, Ohta H. Enhancement of extraplastidic oil synthesis in Chlamydomonas reinhardtii using a type-2 diacylglycerol acyltransferase with a phosphorus starvation-inducible promoter. Plant Biotechnol J. 2014;12(6):808–19.
Article
CAS
PubMed
PubMed Central
Google Scholar
Maul JE, Lilly JW, Cui LY, dePamphilis CW, Miller W, Harris EH, Stern DB. The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats. Plant Cell. 2002;14(11):2659–79.
Article
CAS
PubMed
PubMed Central
Google Scholar
Specht E, Miyake-Stoner S, Mayfield S. Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett. 2010;32(10):1373–83.
Article
CAS
PubMed
PubMed Central
Google Scholar
Doron L, Segal N, Shapira M. Transgene expression in microalgae-from tools to applications. Front Plant Sci. 2016;7:505.
Article
PubMed
PubMed Central
Google Scholar
Gong YM, Hu HH, Gao Y, Xu XD, Gao H. Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects. J Ind Microbiol Biotech. 2011;38(12):1879–90.
Article
CAS
Google Scholar
Erpel F, Restovic F, Arce-Johnson P. Development of phytase-expressing Chlamydomonas reinhardtii for monogastric animal nutrition. BMC Biotechnol. 2016;16:29.
Article
PubMed
PubMed Central
CAS
Google Scholar
Bock R. Engineering plastid genomes: methods, tools, and applications in basic research and biotechnology. Annu Rev Plant Biol. 2015;66:211–41.
Article
CAS
PubMed
Google Scholar
Olejniczak SA, Lojewska E, Kowalczyk T, Sakowicz T. Chloroplasts: state of research and practical applications of plastome sequencing. Planta. 2016;244(3):517–27.
Article
CAS
PubMed
PubMed Central
Google Scholar
Hanson MR, Gray BN, Ahner BA. Chloroplast transformation for engineering of photosynthesis. J Exp Bot. 2013;64(3):731–42.
Article
CAS
PubMed
Google Scholar
Braun-Galleani S, Baganz F, Purton S. Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid verde fluorescent protein as a reporter. Biotechnol J. 2015;10(8):1289–97.
Article
CAS
PubMed
PubMed Central
Google Scholar
Mayfield SP, Schultz J. Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast. Plant J. 2004;37(3):449–58.
Article
CAS
PubMed
Google Scholar
Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield SP. Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Mol Genet Genom. 2005;274(6):625–36.
Article
CAS
Google Scholar
Eberhard S, Drapier D, Wollman FA. Searching limiting steps in the expression of chloroplast-encoded proteins: relations between gene copy number, transcription, transcript abundance and translation rate in the chloroplast of Chlamydomonas reinhardtii. Plant J. 2002;31(2):149–60.
Article
CAS
PubMed
Google Scholar
Manuell AL, Beligni MV, Elder JH, Siefker DT, Tran M, Weber A, McDonald TL, Mayfield SP. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. Plant Biotechnol J. 2007;5(3):402–12.
Article
CAS
PubMed
Google Scholar
Rasala BA, Muto M, Lee PA, Jager M, Cardoso RM, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield SP. Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 2010;8(6):719–33.
Article
CAS
PubMed
PubMed Central
Google Scholar
Surzycki R, Greenham K, Kitayama K, Dibal F, Wagner R, Rochaix JD, Ajam T, Surzycki S. Factors effecting expression of vaccines in microalgae. Biologicals. 2009;37(3):133–8.
Article
CAS
PubMed
Google Scholar
Rasala BA, Muto M, Sullivan J, Mayfield SP. Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5′ untranslated region optimization. Plant Biotechnol J. 2011;9(6):674–83.
Article
CAS
PubMed
Google Scholar
Muto M, Henry RE, Mayfield SP. Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast. BMC Biotechnol. 2009;9(1):26.
Article
PubMed
PubMed Central
CAS
Google Scholar
Kuroda H, Maliga P. Sequences downstream of the translation initiation codon are important determinants of translation efficiency in chloroplasts. Plant Physiol. 2001;125(1):430–6.
Article
CAS
PubMed
PubMed Central
Google Scholar
Gray BN, Ahner BA, Hanson MR. High-level bacterial cellulase accumulation in chloroplast-transformed tobacco mediated by downstream box fusions. Biotechnol Bioeng. 2008;102(4):1045–54.
Article
CAS
Google Scholar
Gray BN, Yang HJ, Ahner BA, Hanson MR. An efficient downstream box fusion allows high-level accumulation of active bacterial beta-glucosidase in tobacco chloroplasts. Plant Mol Biol. 2011;76(3–5):345–55.
Article
CAS
PubMed
Google Scholar
Paulova L, Patakova P, Branska B, Rychtera M, Melzoch K. Lignocellulosic ethanol: technology design and its impact on process efficiency. Biotechnol Adv. 2015;33(6):1091–107.
Article
CAS
PubMed
Google Scholar
Togasaki RK, Brunke K, Kitayama M, Griffith M. Isolation of intact chloroplasts from Chlamydomonas reinhardtii with Beckman centrifugal elutriation system. In: Biggins J, editor. Progress in photosynthesis research, vol. 3. Dordrecht: Springer; 1987. p. 499–502.
Chapter
Google Scholar
Hunter SH. Organic growth essentials of the aerobic nonsulfur photosynthetic bacteria. J Bacteriol. 1946;52(2):213–21.
CAS
PubMed
PubMed Central
Google Scholar
Cerutti H, Johnson AM, Gillham NW, Boynton JE. A eubacterial gene conferring spectinomycin resistance on Chlamydomonas reinhardtii: integration into the nuclear genome and gene expression. Genetics. 1997;145(1):97–110.
CAS
PubMed
PubMed Central
Google Scholar
Ramesh VM, Bingham SE, Webber AN. A simple method for chloroplast transformation in Chlamydomonas reinhardtii. Methods Mol Biol. 2004;274:301–7.
CAS
PubMed
Google Scholar
Guzman-Zapata D, Macedo-Osorio KS, Almaraz-Delgado AL, Duran-Figueroa N, Badillo-Corona JA. Production of recombinant proteins in the chloroplast of the green alga Chlamydomonas reinhardtii. Methods Mol Biol. 2016;1385:69–85.
Article
CAS
PubMed
Google Scholar
Noor-Mohammadi S, Pourmir A, Johannes TW. Method to assemble and integrate biochemical pathways into the chloroplast genome of Chlamydomonas reinhardtii. Biotechnol Bioeng. 2012;109(11):2896–903.
Article
CAS
PubMed
Google Scholar
Diaz-Troya S, Florencio FJ, Crespo JL. Target of rapamycin and LST8 proteins associate with membranes from the endoplasmic reticulum in the unicellular green alga Chlamydomonas reinhardtii. Eukaryot Cell. 2008;7(2):212–22.
Article
CAS
PubMed
Google Scholar
Rodolfi L, Zittelli GC, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng. 2009;102(1):100–12.
Article
CAS
PubMed
Google Scholar
Miller R, Wu GX, Deshpande RR, Vieler A, Gartner K, Li XB, Moellering ER, Zauner S, Cornish AJ, Liu BS, et al. Changes in transcript abundance in Chlamydomonas reinhardtii following nitrogen deprivation predict diversion of metabolism. Plant Physiol. 2010;154(4):1737–52.
Article
CAS
PubMed
PubMed Central
Google Scholar
Yap BH, Crawford SA, Dagastine RR, Scales PJ, Martin GJ. Nitrogen deprivation of microalgae: effect on cell size, cell wall thickness, cell strength, and resistance to mechanical disruption. J Ind Microbiol Biotechnol. 2016;43(12):1671–80.
Article
CAS
PubMed
Google Scholar
Harun R, Singh M, Forde GM, Danquah MK. Bioprocess engineering of microalgae to produce a variety of consumer products. Renew Sust Energ Rev. 2010;14(3):1037–47.
Article
CAS
Google Scholar
Hannon M, Gimpel J, Tran M, Rasala B, Mayfield S. Biofuels from algae: challenges and potential. Biofuels. 2010;1(5):763–84.
Article
CAS
PubMed
PubMed Central
Google Scholar
Mansfeldt CB, Richter LV, Ahner BA, Cochlan WP, Richardson RE. Use of de novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols. PLoS ONE. 2016;11(2):e0147527.
Article
PubMed
PubMed Central
CAS
Google Scholar
Coragliotti AT, Beligni MV, Franklin SE, Mayfield SP. Molecular factors affecting the accumulation of recombinant proteins in the Chlamydomonas reinhardtii chloroplast. Mol Biotechnol. 2011;48(1):60–75.
Article
CAS
PubMed
Google Scholar
Siaut M, Cuine S, Cagnon C, Fessler B, Nguyen M, Carrier P, Beyly A, Beisson F, Triantaphylides C, Li-Beisson YH, Peltier G. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnol. 2011;11(1):7.
Article
CAS
PubMed
PubMed Central
Google Scholar
Sager R, Granick S. Nutritional control of sexuality in Chlamydomonas reinhardtii. J Gen Physiol. 1954;37(6):729–42.
Article
CAS
PubMed
PubMed Central
Google Scholar