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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids

Fig. 3

Coexpression of RoFadD1 and RoTetRs along with tDGAT and RoPAP further increased TAG synthesis. Cells were cultured in ZYP-5052 auto-induction medium at 37 °C with shaking at 200 rpm for 48 h. Thin-layer chromatography (TLC) was carried out on lipids extracted from 5 mg of dried cells. tDGAT, WS/DGAT from T. curvata, RoPAP, PAP from R. opacus PD630. RoFadD1, putative acyl-CoA synthetase from R. opacus PD630. FadR, fatty acid metabolism regulator from E. coli MG1655; RoTetR1/2/3, three putative fatty acid metabolism regulators from R. opacus PD630. All data are the means ± standard deviations from triplicates

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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