Table 1 Strains and plasmids used in this study

EZ-T™

A cloning vector transformed from pBlueScript II SK(+)

NA

GenStar

pCP3031

A suicide vector integrated between slr2030 and slr2031

NA

[24]

E. coli DH5α

F⁻, φ80d lacZ∆M15, ∆ (lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rk⁻, mk⁺), phoA, supE44, λ-, thi-1, gyrA96, relA1

NA

TransGen Biotech

WT

Wild-type Synechocystis sp. PCC 6803

NA

ATCC 27184

ALE-7.0

A evolved strain just tolerant to 7.0-µM CdSO₄

NA

This study

ALE-9.0

End-point strain of ALE

NA

This study

∆slr0454 ^WT

WT-∆slr0454::cm^r

NA

This study

∆slr0623 ^WT

WT-∆slr0623::cm^r

NA

This study

∆slr0721 ^WT

WT-∆slr0721::cm^r

NA

This study

∆slr0774 ^WT

WT-∆slr0774::cm^r

NA

This study

∆slr0798 ^WT

WT-∆slr0798::cm^r

NA

This study

∆slr1302 ^WT

WT-∆slr1302::cm^r

NA

This study

∆ssr1480 ^WT

WT-∆ssr1480::cm^r

NA

This study

∆sll1586 ^WT

WT-∆sll1586::cm^r

NA

This study

∆slr1753 ^WT

WT-∆slr1753::cm^r

NA

This study

WT-C

WT-∆slr0168::cm^r

NA

This study

∆slr0454 ^ALE-9.0

ALE-9.0-∆slr0454::cm^r

NA

This study

∆slr0623 ^ALE-9.0

ALE-9.0-∆slr0623::cm^r

NA

This study

∆slr0721 ^ALE-9.0

ALE-9.0-∆slr0721::cm^r

NA

This study

∆slr0774 ^ALE-9.0

ALE-9.0-∆slr0774::cm^r

NA

This study

∆slr0798 ^ALE-9.0

ALE-9.0-∆slr0798::cm^r

NA

This study

∆slr1302 ^ALE-9.0

ALE-9.0-∆slr1302::cm^r

NA

This study

∆ssr1480 ^ALE-9.0

ALE-9.0-∆ssr1480::cm^r

NA

This study

∆sll1586 ^ALE-9.0

ALE-9.0-∆sll1586::cm^r

NA

This study

∆slr1753 ^ALE-9.0

ALE-9.0-∆slr1753::cm^r

NA

This study

ALE-9.0-C

ALE-9.0-∆slr0168::cm^r

NA

This study

OE-slr0454^WT

∆slr2030–slr2031:: P_cpc560_ slr0454^WT _T_rbcl spe^r

XhoI/NdeI

This study

OE-slr0623^WT

∆slr2030–slr2031:: P_cpc560_ slr0623^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0721^WT

∆slr2030–slr2031:: P_cpc560_ slr0721^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0774^WT

∆slr2030–slr2031:: P_cpc560_ slr0774^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0798^WT

∆slr2030–slr2031:: P_cpc560_ slr0798^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr1302^WT

∆slr2030–slr2031:: P_cpc560_ slr1302^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-ssr1480^WT

∆slr2030–slr2031:: P_cpc560_ ssr1480^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-sll1586^WT

∆slr2030–slr2031:: P_cpc560_ sll1586^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr1753^WT

∆slr2030–slr2031:: P_cpc560_ slr1753^WT _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0454^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr0454^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0623^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr0623^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0721^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr0721^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0774^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr0774^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr0798^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr0798^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr1302^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr1302^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-ssr1480^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ ssr1480^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-sll1586^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ sll1586^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-slr1753^ALE-9.0

∆slr2030–slr2031:: P_cpc560_ slr1753^ALE-9.0 _T_rbcl spe^r

XhoI/BglII

This study

OE-C

∆slr2030–slr2031:: P_cpc560_T_rbcl spe^r

NA

This study