Skip to main content

Table 2 Metabolic engineering of microorganisms for ethylene production

From: Bio-production of gaseous alkenes: ethylene, isoprene, isobutene

Organism Description Production References
Escherichia coli MG1655 efe cloned into a high-copy plasmid downstream of a lac inducible promoter 4.39e−5 mL−1 A600−1 mL−1 (peak production) [3]
Escherichia coli JM109 Transformed with efe from P. syringae on pUC19 backbone 0.23 mL L−1 h−1 [34]
Escherichia coli JM109 Overexpressed EFE from P. syringae 10 mL h−1 g−1 dcw [86]
Synechocystis sp. PCC 6803 Overexpressed EFE from P. syringae and blocked TCA intermediate enzymes 9.7 mL L−1 h−1 [32]
Synechocystis sp. PCC 6803 Genome integration of efe 0.44 mL L−1 h−1 [87]
Synechocystis sp. PCC 6803 4 copies of efe, modified TCA cycle, and partial deletion of ntcA 2.64 mL L−1 h−1 A730−1 [42]
Synechocystis sp. PCC 6803 Chromosomal insertion of codon-optimized efe from P. syringae under pea pshA promoter 5.65 mL L−1 h−1 [30]
Synechocystis sp. PCC 6803 Integrated efe cassette with modified ribosomal binding site 0.72 mL L−1 h−1 A −1730 [31]
Synechocystis sp. PCC 6803 Codon-optimized efe expressed from pVZ321 with lacI repressor, Trc promoter, pol-linker, and transcription terminators from pTrc99a 0.2 mL L−1 h−1 [15]
Saccharomyces cerevisiae Multicopy plasmid expression of oxidase Aox1 and nox 0.23 mL L−1 h−1 [88]
Saccharomyces cerevisiae efe cloned into pYX212-EFE under TPI1 promoter 0.19 mL L−1 h−1 [89]
Saccharomyces cerevisiae Expressed P. syringae efe 0.34 mL L−1 h−1 [39]
Synechococcus sp. PCC 7942 efe cloned in pUC303 with promoter and terminator from psbAI 0.03 mL L−1 h−1 A730−1 [90]
Synechococcus elongatus PCC 7942 P. syringae efe was inserted at the psbAI locus 0.451 mL L−1 h−1 A730−1 [91]
Trichoderma viride Integrated efe from P. syringae into chromosome under the control of cbh1 promoter 0.00106 mL h−1 g−1 dcw [92]
Trichoderma reesei Integrated into chromosome 4 efe genes under pgkL promoter 0.0041 mL L−1 h−1 [33]
Pseudomonas syringae Cloned efe into a high-copy plasmid pMEFE1 8 mL L−1 h−1 A610−1 [93]
Pseudomonas putida KT2440 Integrated efe from P. syringae at five 16S rDNA sites 64.04 mL h−1 g−1 dcw [41]