Table 2 Metabolic engineering of microorganisms for ethylene production

Escherichia coli MG1655

efe cloned into a high-copy plasmid downstream of a lac inducible promoter

4.39e⁻⁵ mL⁻¹ A₆₀₀⁻¹ mL⁻¹ (peak production)

[3]

Escherichia coli JM109

Transformed with efe from P. syringae on pUC19 backbone

0.23 mL L⁻¹ h⁻¹

[34]

Escherichia coli JM109

Overexpressed EFE from P. syringae

10 mL h⁻¹ g⁻¹ dcw

[86]

Synechocystis sp. PCC 6803

Overexpressed EFE from P. syringae and blocked TCA intermediate enzymes

9.7 mL L⁻¹ h⁻¹

[32]

Synechocystis sp. PCC 6803

Genome integration of efe

0.44 mL L⁻¹ h⁻¹

[87]

Synechocystis sp. PCC 6803

4 copies of efe, modified TCA cycle, and partial deletion of ntcA

2.64 mL L⁻¹ h⁻¹ A₇₃₀⁻¹

[42]

Synechocystis sp. PCC 6803

Chromosomal insertion of codon-optimized efe from P. syringae under pea pshA promoter

5.65 mL L⁻¹ h⁻¹

[30]

Synechocystis sp. PCC 6803

Integrated efe cassette with modified ribosomal binding site

0.72 mL L⁻¹ h⁻¹ A ⁻¹₇₃₀

[31]

Synechocystis sp. PCC 6803

Codon-optimized efe expressed from pVZ321 with lacI repressor, Trc promoter, pol-linker, and transcription terminators from pTrc99a

0.2 mL L⁻¹ h⁻¹

[15]

Saccharomyces cerevisiae

Multicopy plasmid expression of oxidase Aox1 and nox

0.23 mL L⁻¹ h⁻¹

[88]

Saccharomyces cerevisiae

efe cloned into pYX212-EFE under TPI1 promoter

0.19 mL L⁻¹ h⁻¹

[89]

Saccharomyces cerevisiae

Expressed P. syringae efe

0.34 mL L⁻¹ h⁻¹

[39]

Synechococcus sp. PCC 7942

efe cloned in pUC303 with promoter and terminator from psbAI

0.03 mL L⁻¹ h⁻¹ A₇₃₀⁻¹

[90]

Synechococcus elongatus PCC 7942

P. syringae efe was inserted at the psbAI locus

0.451 mL L⁻¹ h⁻¹ A₇₃₀⁻¹

[91]

Trichoderma viride

Integrated efe from P. syringae into chromosome under the control of cbh1 promoter

0.00106 mL h⁻¹ g⁻¹ dcw

[92]

Trichoderma reesei

Integrated into chromosome 4 efe genes under pgkL promoter

0.0041 mL L⁻¹ h⁻¹

[33]

Pseudomonas syringae

Cloned efe into a high-copy plasmid pMEFE1

8 mL L⁻¹ h⁻¹ A₆₁₀⁻¹

[93]

Pseudomonas putida KT2440

Integrated efe from P. syringae at five 16S rDNA sites

64.04 mL h⁻¹ g⁻¹ dcw

[41]