Fig. 2
From: Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
Gene deletion and point mutation in O. polymorpha. a Editing efficiencies for deletions of genes OpURA3 and OpLEU2. b Editing efficiencies of OpADE2 deletion using 50- to 1000-bp length of homologous arms. c Editing efficiencies of multi-locus deletions of three genes OpLEU2, OpURA3 and OpHIS3. d The editing efficiency of point mutation in the gene OpURA3. NA: no correct mutant was obtained. Error bars indicate standard deviations of three biological repeats (n = 3)