Fig. 2
FACS sorting of +N N. oceanica cultures and proteomic abundance differences on triacylglycerol pathway. a Autofluorescence control for unstained +N N. oceanica cells, control for cells which were incubated in 15% DMSO and control cells incubated with 0.7 µg/ml Nile Red without DMSO. b Cell density plot +N N. oceanica cells stained with Nile Red, the SSC (Sidescatter)-height channel informs about cell size and morphological features, while the Nile Red intensity axis value reports the intensity of Nile Red fluorescence detected through FL2-Log-Height fluorescence channel. Percentage in upper left corner indicates percentage of depicted events from total. Percentages above subpopulations denote gated cell fraction from total. c Diagram of regulation of enzymes belonging to the carbon fixation towards triacylglycerol pathway between +N P3 and +N P4 subpopulations. Metabolites are highlighted by gray boxes and enzymes are highlighted by yellow boxes, color of the arrows indicates up- or down-regulation, while the numbers adjacent to the enzyme names are the regulation factors (log2). Rb-1,5-P: Ribulose-1,5-bisphosphate; RUBISCO: ribulose-1,5-bisphosphate carboxylase; cbbx: cbbx homolog to protein activating expression of Rubisco; G3P: 3-phospho glycerate; F-1,6-P: fructose-1,6-bisphosphate; GAP: glycerine aldehyde-3-phosphate; DHAP: dihydroxyacetone-phosphate; PEP: phosphoenolpyruvate; Malonyl-CoA: Malonyl-coezyme A; Malonyl-ACP: Malonyl-acyl carrier protein; Acyl-CoA: acyl-coenzyme A; LACS: Long chain acyl CoA synthetase; LPAAT: lysophosphatidic acid acyltransferase; LPAT: lyso-phosphatidylcholine acyltransferase; DGAT: diacylglycerol acetyltransferase; ER: endoplasmic reticulum; OAA: oxaloacetate; TAG: triacylglycerol; LDSP: lipid droplet surface protein; M6PR: NADPH dependent Mannose-6-phosphate reductase; PEP: phosphoenolpyruvate; OAA: oxaloacetate; PPDK: pyruvate, phosphate dikinase; TIM: triosephosphate isomerase. CO2 fixation by Rubisco and fatty acid biosynthesis occurs in plastid, carbon fixation involving PPDK in cytosol, TAG synthesis in ER and storage of TAG in lipid bodies (filled yellow)