Hewitt CJ, Nebe-Von Caron G, Nienow AW, McFarlane CM. Use of multi-staining flow cytometry to characterise the physiological state of Escherichia coli W3110 in high cell density fed-batch cultures. Biotechnol Bioeng. 1999;63(6):705–11.
Article
CAS
PubMed
Google Scholar
Want A, Thomas ORT, Kara B, Liddell J, Hewitt CJ. Studies related to antibody fragment (Fab) production in Escherichia coli W3110 fed-batch fermentation processes using multiparameter flow cytometry. Cytometry Part A. 2009;75A(2):148–54.
Article
CAS
Google Scholar
Höfler C, Heckmann J, Fritsch A, Popp P, Gebhard S, Fritz G, Mascher T. Cannibalism stress response in Bacillus subtilis. Microbiology. 2016;162(1):164–76.
Article
PubMed
CAS
Google Scholar
Moormeier DE, Bayles KW. Staphylococcus aureus biofilm: a complex developmental organism. Mol Microbiol. 2017;104(3):365–76.
Article
CAS
PubMed
PubMed Central
Google Scholar
Mustafi N, Grünberger A, Mahr R, Helfrich S, Nöh K, Blombach B, Kohlheyer D, Frunzke J. Application of a genetically encoded biosensor for live cell imaging of l-valine production in pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum strains. PLoS ONE. 2014;9(1):e85731.
Article
PubMed
PubMed Central
CAS
Google Scholar
Frunzke J, Bramkamp M, Schweitzer J-E, Bott M. Population heterogeneity in Corynebacterium glutamicum ATCC 13032 caused by prophage CGP3. J Bacteriol. 2008;190(14):5111–9.
Article
CAS
PubMed
PubMed Central
Google Scholar
Di L, Evans T, Zhang F. Applications and advances of metabolite biosensors for metabolic engineering. Metab Eng. 2015;31:35–43.
Article
CAS
Google Scholar
Harst A, Albaum SP, Bojarzyn T, Trotschel C, Poetsch A. Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations. J Proteom. 2017;160:1–7.
Article
CAS
Google Scholar
Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger K-E, Kohlheyer D, Drepper T. Comparative single-cell analysis of different E. coli expression systems during microfluidic cultivation. PLoS ONE. 2016;11(8):e0160711.
Article
PubMed
PubMed Central
CAS
Google Scholar
Lindmeyer M, Jahn M, Vorpahl C, Müller S, Schmid A, Bühler B. Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains. Front Microbiol. 2015;6:1042.
Article
PubMed
PubMed Central
Google Scholar
Ji P, Zhang Y, Wang J, Zhao F. MetaSort untangles metagenome assembly by reducing microbial community complexity. Nat Commun. 2017;8:14306.
Article
CAS
PubMed
PubMed Central
Google Scholar
Dong HP, Williams E, Wang DZ, Xie ZX, Hsia RC, Jenck A, Halden R, Li J, Chen F, Place AR. Responses of Nannochloropsis oceanica IMET1 to long-term nitrogen starvation and recovery. Plant Physiol. 2013;162(2):1110–26.
Article
CAS
PubMed
PubMed Central
Google Scholar
Sukenik A, Carmeli Y, Berner T. Regulation of fatty acid composition by irradiance level in the eustigmatophyte Nannochloropsis sp. J Phycol. 1989;25(4):686–92.
Article
CAS
Google Scholar
Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR. Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng. 2009;102(1):100–12.
Article
CAS
PubMed
Google Scholar
Li J, Han D, Wang D, Ning K, Jia J, Wei L, Jing X, Huang S, Chen J, Li Y, et al. Choreography of transcriptomes and lipidomes of nannochloropsis reveals the mechanisms of oil synthesis in microalgae. Plant Cell. 2014;26(4):1645–65.
Article
CAS
PubMed
PubMed Central
Google Scholar
Wang T, Ji Y, Wang Y, Jia J, Li J, Huang S, Han D, Hu Q, Huang WE, Xu J. Quantitative dynamics of triacylglycerol accumulation in microalgae populations at single-cell resolution revealed by Raman microspectroscopy. Biotechnol Biofuels. 2014;7:58.
Article
PubMed
PubMed Central
CAS
Google Scholar
Doan T-TY, Obbard JP. Improved Nile red staining of Nannochloropsis sp. J Appl Phycol. 2011;23(5):895–901.
Article
CAS
Google Scholar
Yen Doan T-T, Obbard JP. Enhanced lipid production in Nannochloropsis sp. using fluorescence-activated cell sorting. GCB Bioenergy. 2011;3(3):264–70.
Article
CAS
Google Scholar
Rumin J, Bonnefond H, Saint-Jean B, Rouxel C, Sciandra A, Bernard O, Cadoret J-P, Bougaran G. The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae. Biotechnol Biofuels. 2015;8:42.
Article
PubMed
PubMed Central
CAS
Google Scholar
Lee D-H, Bae CY, Han J-I, Park J-K. In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules. Anal Chem. 2013;85(18):8749–56.
Article
CAS
PubMed
Google Scholar
Traller JC, Hildebrand M. High throughput imaging to the diatom Cyclotella cryptica demonstrates substantial cell-to-cell variability in the rate and extent of triacylglycerol accumulation. Algal Res. 2013;2(3):244–52.
Article
Google Scholar
Su J, Yang X, Zheng T, Hong H. An efficient method to obtain axenic cultures of Alexandrium tamarense—a PSP-producing dinoflagellate. J Microbiol Methods. 2007;69(3):425–30.
Article
CAS
PubMed
Google Scholar
Guillard RRL. Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH, editors. Culture of marine invertebrate animals: proceedings—1st conference on culture of marine invertebrate animals greenport. Boston, MA: Springer US; 1975:29–60.
Jehmlich N, Hübschmann T, Gesell Salazar M, Völker U, Benndorf D, Müller S, Bergen M, Schmidt F. Advanced tool for characterization of microbial cultures by combining cytomics and proteomics. Appl Microbiol Biotechnol. 2010;88(2):575–84.
Article
CAS
PubMed
Google Scholar
Cox J, Mann M. MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. Nat Biotechnol. 2008;26(12):1367–72.
Article
CAS
PubMed
Google Scholar
Cox J, Hein MY, Luber CA, Paron I, Nagaraj N, Mann M. Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, Termed MaxLFQ. Mol Cell Proteom. 2014;13(9):2513–26.
Article
CAS
Google Scholar
Vizcaino JA, Csordas A, Del-Toro N, Dianes JA, Griss J, Lavidas I, Mayer G, Perez-Riverol Y, Reisinger F, Ternent T, et al. 2016 update of the PRIDE database and its related tools. Nucleic Acids Res. 2016;44(22):11033.
Article
CAS
PubMed
PubMed Central
Google Scholar
Dray S, Dufour A. The ade4 Package: implementing the duality diagram for ecologists. J Stat Softw. 2007;22(4):1–20.
Article
Google Scholar
Terashima M, Freeman ES, Jinkerson RE, Jonikas MC. A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants. Plant J Cell Mol Biol. 2015;81(1):147–59.
Article
CAS
Google Scholar
Guzmán HM, Valido ADLJ, Presmanes KF, Duarte LC. Quick estimation of intraspecific variation of fatty acid composition in Dunaliella salina using flow cytometry and Nile Red. J Appl Phycol. 2012;24(5):1237–43.
Article
CAS
Google Scholar
Lubke GH, Muthén B. Investigating population heterogeneity with factor mixture models. Psychol Methods. 2005;10(1):21.
Article
PubMed
Google Scholar
Müller S, Harms H, Bley T. Origin and analysis of microbial population heterogeneity in bioprocesses. Curr Opin Biotechnol. 2007;59(11):839–52.
Google Scholar
Hyka P, Lickova S, Přibyl P, Melzoch K, Kovar K. Flow cytometry for the development of biotechnological processes with microalgae. Biotechnol Adv. 2013;31(1):2.
Article
CAS
PubMed
Google Scholar
Yoneda K, Yoshida M, Suzuki I, Watanabe MM. Identification of a major lipid droplet protein in a marine diatom Phaeodactylum tricornutum. Plant Cell Physiol. 2016;57(2):397–406.
Article
CAS
PubMed
Google Scholar
Clement R, Dimnet L, Maberly SC, Gontero B. The nature of the CO2-concentrating mechanisms in a marine diatom, Thalassiosira pseudonana. New Phytol. 2016;209(4):1417–27.
Article
CAS
PubMed
Google Scholar
Loganathan N, Tsai Y-CC, Mueller-Cajar O. Characterization of the heterooligomeric red-type rubisco activase from red algae. Proc Natl Acad Sci. 2016;113(49):14019–24.
Article
CAS
PubMed
Google Scholar
Stoop J, Pharr DM. Mannitol metabolism in celery stressed by excess macronutrients. Plant Physiol. 1994;106(2):503–11.
Article
CAS
PubMed
PubMed Central
Google Scholar
Bellou S, Baeshen MN, Elazzazy AM, Aggeli D, Sayegh F, Aggelis G. Microalgal lipids biochemistry and biotechnological perspectives. Biotechnol Adv. 2014;32(8):1476–93.
Article
CAS
PubMed
Google Scholar
Ho S-H, Chang J-S, Lai Y-Y, Chen C-NN. Achieving high lipid productivity of a thermotolerant microalga Desmodesmus sp. F2 by optimizing environmental factors and nutrient conditions. Bioresour Technol. 2014;156:108–16.
Article
CAS
PubMed
Google Scholar
Alipanah L, Rohloff J, Winge P, Bones AM, Brembu T. Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum. J Exp Bot. 2015;66(20):6281–96.
Article
CAS
PubMed
PubMed Central
Google Scholar
Simionato D, Block MA, La Rocca N, Jouhet J, Maréchal E, Finazzi G, Morosinotto T. The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus. Eukaryot Cell. 2013;12(5):665–76.
Article
CAS
PubMed
PubMed Central
Google Scholar
Moellering ER, Benning C. RNA interference silencing of a major lipid droplet protein affects lipid droplet size in Chlamydomonas reinhardtii. Eukaryot Cell. 2010;9(1):97.
Article
CAS
PubMed
Google Scholar
Smith SR, Gle C, Abbriano RM, Traller JC, Davis A, Trentacoste E, Vernet M, Allen AE, Hildebrand M. Transcript level coordination of carbon pathways during silicon starvation-induced lipid accumulation in the diatom Thalassiosira pseudonana. New Phytol. 2016;210(3):890–904.
Article
CAS
PubMed
PubMed Central
Google Scholar
He Y, Zhang P, Huang S, Wang T, Ji Y, Xu J. Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells. Biotechnol Biofuels. 2017;10:275.
Article
PubMed
PubMed Central
CAS
Google Scholar
Wakisaka Y, Suzuki Y, Iwata O, Nakashima A, Ito T, Hirose M, Domon R, Sugawara M, Tsumura N, Watarai H, et al. Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy. Nat Microbiol. 2016;1(10):16124.
Article
CAS
PubMed
Google Scholar
Havlin S, Buldyrev SV, Goldberger AL, Mantegna RN, Ossadnik SM, Peng CK, Simons M, Stanley HE. Fractals in biology and medicine. Chaos Solitons Fractals. 1995;6(1):171–201.
Article
CAS
PubMed
Google Scholar
Coffey DS. Self-organization, complexity and chaos: the new biology for medicine. Nat Med. 1998;4(8):882.
Article
CAS
PubMed
Google Scholar
Davey HM, Kell DB. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses. Microbiol Rev. 1996;60(4):641–96.
CAS
PubMed
PubMed Central
Google Scholar
Lidstrom ME, Konopka MC. The role of physiological heterogeneity in microbial population behavior. Nat Chem Biol. 2010;6(10):705.
Article
CAS
PubMed
Google Scholar