Fig. 2

Characterization of Arabidopsis fpgs1, ccoaomt1, and fpgs1ccoaomt1 mutants. a Schematic diagram of the exon–intron organization of CCoAOMT1 and FPGS1 genes, and the T-DNA insertion positions in ccoaomt1 (SALK_151507) and fpgs1 mutants. b Confirmation of FPGS1 and CCoAOMT1 transcript levels in 9-day-old plate-grown WT, fpgs1, ccoaomt1and fpgs1ccoaomt1 by RT-PCR (Lane M, 1 kb DNA ladder Promega G5711; lane 1, housekeeping control AtACTIN 2 gene; lane 2, FPGS1 gene; lane 3, CCoAOMT1 gene). c 6-week-old WT, fpgs1(f1), ccoaomt1 (cc1), and fpgs1ccoaomt1 (f1cc1) Arabidopsis plants prior sampling the stems for analysis (n ≥ 30). d Comparisons of plant height, fresh stem weight, and fresh aboveground weight of 6-week-old WT, fpgs1, ccoaomt1, and fpgs1ccoaomt1 Arabidopsis plants. Plant height is the height of the primary inflorescence stem; Aboveground plant fresh weight: include all aboveground tissues including leaves, flowers, and siliques; Plant stem fresh weight: aboveground tissue after removal of rosette leaves, cauline leaves, flowers, and siliques. The data were collected from 30 plants for each genotype. There were no statistically significant differences between values according to one-way ANOVA and LSD test (values were mean ± SE. n = 30, P ≤ 0.05)