Fig. 3

Intracellular expression of CglT enhanced cellobiose utilisation. a Western blot showing FLAG-tagged CglT using ANTI-FLAG M2 monoclonal antibody-horseradish peroxidise conjugate. Lane Wt. and CglT represents soluble fraction of cell lysate from wild-type G. thermoglucosidasius and the recombinant G. thermoglucosidasius ZB3bInt strain, respectively. Lane M is pre-stained protein ladder (10–250 kDa); Expected molecular mass of 50 kDa for CglT is indicated by red arrow. b pNPGase specific activity of recombinant G. thermoglucosidasius ZB3bInt strains (ZB3bInt) and wild-type G. thermoglucosidasius strains (WT). P values were calculated by student’s t test and results are shown as mean ± SEM of three biological replicates. c Cellobiose consumption and growth profiles by recombinant G. thermoglucosidasius ZB3bInt (ZB3bInt) and wild-type G. thermoglucosidasius strains (WT) when grown on 3.0% cellobiose as the carbon source at 55 °C. Black circles, G. thermoglucosidasius_ZB3bInt (OD600 nm); Red circles, wild-type (OD600 nm); blue square, G. thermoglucosidasius_Zb3bInt (remaining cellobiose); green squares, wild-type (remaining cellobiose). Results are shown as mean ± SEM of three biological replicates