Fig. 2

Enzymatic conversion of a l-fucose to l-fuculose (forward reaction) and b l-fuculose to l-fucose (reverse reaction) by RdFucI. Enzymatic reaction was performed by 3 µg of RdFucI with either a l-fucose or b l-fuculose at 10 mM as the starting substrate at 30 °C for 10 min in 20 mM sodium phosphate (pH 7.0) in the presence of 1 mM of Mn²⁺. l-Fucose concentration was experimentally measured and l-fuculose concentration was calculated by subtracting experimentally determined l-fucose concentration from total sugar concentration (10 mM). Experimental data represent means ± standard deviations of three replicates