Fig. 3

Effect of a temperature and b pH on the relative activity of RdFucI against l-fuculose. Enzymatic reactions were performed a at various temperatures ranging from 10 to 80 °C and b at various pHs ranging from 4 to 11. The buffers used were 50 mM sodium acetate (pH 4, 5, and 6), 50 mM sodium phosphate (pH 6, 7, and 8), 50 mM Tris–HCl (pH 7, 8, and 9), and 50 mM glycine–NaOH (9, 10, and 11). Experimental data represent means ± standard deviations of three replicates