Skip to main content

Table 2 Kinetic parameters of CkGE15A on various synthetic substrates

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

ConditionTemperature (°C)Km (mM)kcat (1/min)kcat/Km (s−1M−1)
Benzyl-d-glucuronoate2218.5 ± 0.71.18 ± 0.041.06 ± 0.05
Allyl-d-glucuronoate22Cannot be saturated up to 40 mM0.21 ± 3.2×10−3
Methyl-d-glucuronoate22Cannot be saturated up to 40 mM0.028 ± 4.6×10−4
Benzyl-d-glucuronoate408.6 ± 0.93.39 ± 0.146.56 ± 0.38
  1. The results are presented as the average of triplicate experiments with standard errors