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Table 2 Kinetic parameters of CkGE15A on various synthetic substrates

From: Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules

Condition

Temperature (°C)

Km (mM)

kcat (1/min)

kcat/Km (s−1M−1)

Benzyl-d-glucuronoate

22

18.5 ± 0.7

1.18 ± 0.04

1.06 ± 0.05

Allyl-d-glucuronoate

22

Cannot be saturated up to 40 mM

0.21 ± 3.2×10−3

Methyl-d-glucuronoate

22

Cannot be saturated up to 40 mM

0.028 ± 4.6×10−4

Benzyl-d-glucuronoate

40

8.6 ± 0.9

3.39 ± 0.14

6.56 ± 0.38

  1. The results are presented as the average of triplicate experiments with standard errors
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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