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Enhancing methane oxidation in a bioelectrochemical membrane reactor using a soluble electron mediator
Biotechnology for Biofuels volume 13, Article number: 173 (2020)
Bioelectrochemical methane oxidation catalysed by anaerobic methanotrophic archaea (ANME) is constrained by limited methane bioavailability as well as by slow kinetics of extracellular electron transfer (EET) of ANME. In this study, we tested a combination of two strategies to improve the performance of methane-driven bioelectrochemical systems that includes (1) the use of hollow fibre membranes (HFMs) for efficient methane delivery to the ANME organisms and (2) the amendment of ferricyanide, an effective soluble redox mediator, to the liquid medium to enable electrochemical bridging between the ANME organisms and the anode, as well as to promote EET kinetics of ANME.
The combined use of HFMs and the soluble mediator increased the performance of ANME-based bioelectrochemical methane oxidation, enabling the delivery of up to 196 mA m−2, thereby outperforming the control system by 244 times when HFMs were pressurized at 1.6 bar.
Improving methane delivery and EET are critical to enhance the performance of bioelectrochemical methane oxidation. This work demonstrates that by process engineering optimization, energy recovery from methane through its direct oxidation at relevant rates is feasible.
Methane (CH4) is an important energy resource that has attracted increasing attention due to rapidly growing energy demand. CH4 resource is abundant with two sources: fossil natural gas with growing proven reserves, and biogas with renewable availability [1, 2]. Thus CH4 provides us a long-term energy sustainability. Moreover, CH4 is also recognized as a potent greenhouse gas (GHG), with its global-warming potential 28–34 times that of carbon dioxide over a 100-year time frame . It is therefore desirable for direct CH4 utilization or upgrading on-site to minimize its global-warming potential. Both factors sparked interests on developing effective CH4-based technologies to produce energy whilst mitigating its adverse impact on climate change [4, 5].
While direct CH4 combustion in gas turbines has been a widely implemented strategy for energy recovery from CH4, this approach is constrained by the inherently low volumetric energy density for transportation sector and low energy efficiency for electricity generation . Proposed alternatives for CH4 utilization include direct CH4 conversion into electricity, for example in solid fuel cells (SOCFs) [5, 7], and CH4 conversion into liquid fuels (e.g., hydrocarbons) through thermochemical processing [5, 6]. The approach of converting CH4 into electricity in SOCFs is technically challenging due to the high-temperatures required and the instability of the catalysts [5, 8]. Conversely, gas-to-liquid (GTL) technologies such as the well-established Fischer–Tropsch (FT) process, while attractive for its capability to convert CH4 into valuable and energy-dense liquid chemicals and fuels including methanol, longer chain hydrocarbons, olefins, and gasoline, is challenged by high technical complexity as well as numerous heat and pressure changes .
Benefited from increasing awareness of the feasibility of C–H bond activation by biocatalysis, biotechnologies are recently proposed to have good opportunities to support bioconversion of CH4 into electricity or liquid chemicals with virtues of mild operational conditions and technical simplicity over SOCFs and FT process [9, 10]. In this regard, microbial electrochemical technologies have recently been suggested as an effective method for the conversion of CH4 directly into electricity or indirectly into value-added chemicals and fuels through electrochemical conversions catalysed by whole living microbial cells . Bioelectrochemical CH4 transformation into versatile products can overcome its limitation of low volumetric energy density. Bioelectrochemical conversion of CH4 to electricity, compared to direct CH4 combustion in turbine, can achieve a higher efficiency since it avoids the Carnot cycle and produces primarily electricity instead of heat .
While the concept of a CH4-fueled bioelectrochemical systems (BESs) has been speculated ever since the birth of microbial electrochemistry as a research field , the difficulty in finding a suitable biocatalyst displaying abilities of both anaerobic CH4 activation and extracellular electron transfer (EET) has limited the number of successful reports to a handful [5, 13]. Indeed, the demonstration of direct EET as the necessary mechanism to enable CH4 oxidation coupled with sulfate reduction [14, 15], or with insoluble iron and manganese oxides [16, 17] by some lineages of anaerobic methanotrophic archaea (ANME) suggested that ANME might have the ability to respire on solid electrode surfaces. Recently it has been demonstrated that ANME-2d can couple anaerobic oxidation of methane (AOM) to electrode reduction .
While the implications of direct electricity production from CH4 oxidation in BESs at ambient temperature is feasible, the technology is far from large-scale implementations due to the low current densities currently achievable , which are normally orders of magnitude lower than typically achieved by their bacterial electroactive counter parts, e.g., Geobacter . The typically low rates of AOM catalysed by ANME and coupled with electrode reduction in BESs are probably the result of two rate-limiting processes, including (1) poor mass transfer rate and solubility of CH4 in the cultivation media, which limits microbial accessibility to CH4; (2) electron transfer from the microbial cells to the final external electron acceptor (i.e. the anode electrode).
As a possible solution to the poor CH4 mass transfer issue, our group has previously proposed the use of hollow fibre membranes (HFMs) to supply CH4 gas in membrane biofilm reactors (MBfRs) driving denitrification, resulting in an increase in the denitrification performance by 20–30 times relative to a control [21, 22]. This significant improvement can be understood by considering that in MBfRs, methanotrophic biofilm colonize and grow on the outer surface of the membranes, having therefore direct access to CH4 at supersaturation levels. This strategy, while effective when coupling CH4 oxidation with the reduction of a soluble electron acceptor such as nitrate, cannot directly be implemented in BESs where the electron acceptor is a solid-state electrode. To be effective, it is necessary to establish an electrical connection between the methanotrophic biomass on HFMs and the electrode.
In this work, we tested the hypothesis according to which a soluble redox mediator can be used to shuttle electrons between methanotrophic organisms and the anode electrode, thereby “electrochemically bridging” the HFMs (and the biofilm within) and the electrode without the requirement to physically grow the methanotrophic organisms on the surface of the electron-accepting anode. We inoculated our systems with an enriched culture of ANME organisms and amended potassium ferricyanide as the redox mediator. The current knowledge of ANME metabolism when using insoluble electron acceptors points at the involvement of c-type cytochromes in extracellular electron transfer [14, 16, 23, 24]. Therefore, we used a redox mediator with a mid-point potential sufficiently high to effectively extract electrons from c-type cytochromes (E0′ for ferricyanide is ca. + 416 mV vs the Standard Hydrogen Electrode at pH 7 , while the E0′ for c-type cytochromes is reported at ca. − 200 to 300 mV ), as well as for its high electron transfer kinetics at graphite electrodes . We then characterised the bioelectrochemical membrane reactor (BEMR) system in terms of current output and community dynamics and compared the performance with a Control-BES where neither the HFMs nor the mediator was used.
ANME couple methane oxidation with ferricyanide reduction
To validate if ferricyanide can mediate electron transfer from ANME to the electrode, the ability of the ANME-dominated culture to reduce ferricyanide with CH4 as electron donor was firstly investigated. In incubations containing microbial inoculation and CH4 amendment, the characteristic dark yellow colour of ferricyanide in oxidised form gradually faded, and completely disappeared after 48 h (Additional file 1: Fig. S1), yielding a clear uncoloured solution, indicating the progressive reduction of ferricyanide and its conversion into ferrocyanide (Fig. 1a). By comparison, abiotic control (without inoculum) displayed no visible colour change during the incubation, further proving that the ferricyanide reduction observed in the flasks amended with ANME organisms was of a biological nature. Further, the progressive accumulation of 13CO2 indicated 13CH4 oxidation (Fig. 1b), confirming the presence of biological conversions associated with ferricyanide reduction.
Colonization of methanotrophic biofilm on HFMs
Bubble-less gas exchange HFMs have been shown as effective for delivering gaseous substrate of CH4 because CH4 can be delivered directly from the inside of HFMs to a biofilm attached on the outer surface of HFMs [21, 28]. Thus, colonization of a CH4-oxidizing biofilm on HFMs is the prerequisite for a highly efficient CH4 utilization system. As the methanotrophic biomass used here as inoculum was adapted to nitrate as the electron acceptor, we fed nitrate (and ammonium) during Stage 1 of operation to stimulate colonization of methanotrophic biofilm on HFMs. Figure 2a reports the profiles of nitrate and ammonium concentration (and removal rates) over time during multiple batch feeding tests. Nitrate was removed with rates increased over time throughout the operational course of the first 147 days, indicating the gradual colonization of methanotrophic biofilm on the surface of HFMs and confirming our previous observations [21, 29]. Ammonium removal was also observed since the anammox microorganisms contained in the inoculum oxidized ammonium using the nitrite produced by ANME archaea from nitrate reduction, which has been described before . The attachment of methanotrophic biofilm on the HFMs could be also verified by SEM imaging. At the end of operational Stage 1, a dense biofilm morphology can be observed on the outer hollow fibres, a clear indication of the successful colonization (Fig. 2b,c).
Enhanced performance of bioelectrochemical CH4 oxidation in the BEMR
Figure 3 shows CH4-dependent bioelectrochemical current output by means of chronoamperometry in the BEMR (Fig. 3a) and Control-BES (Fig. 3b). The current versus time traces in both systems display initial peaks and maxima in the first 30 days. Coulombic efficiency estimation showed that only 54.3% of electron recovery can be obtained against the assumption that the current output in this period was solely driven by AOM (Additional file 1: Table S1), which suggests that there was some other more oxidized carbonaceous substances contributed to the current output. This could be ascribed to the oxidation of intracellular storage substances present in methanotrophic biomass providing additional reducing equivalents, as previously discussed [30, 31]. Afterwards, the current in the BEMR stabilized at around 149 mA m–2 when the HFMs was pressurized at 0.8 bar. It dropped to approximately 102 mA m−2 as soon as HFMs was depressurized to 0 bar (Fig. 3a, insert). In this scenario, the CH4 supply pressure was the same to the Control-BES, while the current output in the BEMR was still about 126 times higher than the stabilized current (0.8 mA m–2) in the Control-BES. Once the HFMs was re-pressurized at 0.8 bar, the current in the BEMR was quickly recovered to the level of approximately 102 mA m−2, and it was further increased to around 170 mA m−2 and 196 mA m−2 when the pressure applied to HFMs was increased to 1.2 bar and 1.6 bar, respectively.
Regarding the control experiment for confirming the contribution of ferricyanide to increased current output, ferricyanide was gradually removed or replenished to the anode chamber by a manner of medium replacement through continuous pumping. Along with the rapid decline of ferricyanide concentration, the current quickly dropped to around 0.6 mA m−2; as soon as ferricyanide was fed back to the anode chamber, the current rapidly recovered before it plateaued at the original level (Additional file 1: Fig. S3).
The enhancement of AOM in the BEMR was further evidenced by significant CO2 accumulation observed (Additional file 1: Fig. S4). The calculated coulombic efficiency of BEMR was 96.5 ± 4.9% during the stabilized current period (Day 183 to 255) (Additional file 1: Table S1), which suggests that electrons generated from CH4 oxidation can be almost entirely recovered as current output. In comparison, limited CO2 production was observed in the Control-BES (Additional file 1: Fig. S4), which was in line with the low current observation (Fig. 3b).
Electrochemical characterization by cyclic voltammetry
To understand how ferricyanide enhanced bioelectrochemical oxidation, CVs were recorded and compared in the BEMR and Control-BES (Fig. 4). The voltametric response of the Control-BES (insert in Fig. 4) showed a redox couple centred at + 0.17 V, which could be associated with electroactive biofilm on the graphite anode in the Control-BES (as proven by SEM imaging, Additional file 1: Fig. S5). By contrast, the voltammogram of BEMR revealed a reversible redox process featured by ferri- ferrocyanide redox couple. It confirmed the active role of ferricyanide as an effective mediator in the BEMR. The observed higher voltametric current density on the CV profile of the BEMR indicated the excellent electron transfer kinetics of the mediator involved at graphite electrodes, which could facilitate the electron transfer from the microbial cells to the anode ultimately for the enhanced performance of bioelectrochemical oxidation in the BEMR.
Microbial community dynamics
To understand biological processes for bioelectrochemical AOM, 16S rRNA gene amplicon sequencing was undertaken to characterize microbial community dynamics both in the BEMR and the Control-BES at different operational stages (Fig. 5).
The methanotrophic community used as the inoculum for both bioelectrochemical systems described in this work was previously enriched in the presence of nitrate as the final electron acceptor. Accordingly, it contained the ANME archaea Candidatus Methanoperedens (32.8%) and its syntrophic partner anammox bacterium Candidatus Kuenenia (5.2%) . Other bacteria, such as Candidatus Collierbacteria (13.9%) and Rhodocyclaceae (7.0%), also coexist in the community with high percentage, however their roles are yet to be confirmed . At the end of operational Stage 1, the 16S rRNA gene profiling results in the BEMR was in line with the inoculum, which suggested the colonization of methanotrophic biofilms on HFMs. When nitrate was depleted and the BEMR was operated with polarized electrode as the electron acceptor, ANME organism Ca. Methanoperedens still dominated the microbial community (25.7%) at the end of operational Stage 2 in the BEMR. Meanwhile, no other methane-oxidizing microbes were found to occur in the community, revealing that Ca. Methanoperedens was the only candidate being responsible for CH4 activation. Interestingly, organisms belonging to the genus Geobacter were also observed in the community enriched in BEMR, reaching a relative abundance of 2.4%, which could be stimulated by readily available intermediates (such as acetate) released by intracellular storage substances of methanotrophic biomass [30, 31]. Regarding the Control-BES, the microbial community was also consistent with the inoculum when nitrate was fed in the first 147 days. When nitrate was replaced by polarized electrode as electron acceptor, Ca. Methanoperedens also dominated the community without any other CH4- or EET-associated microorganisms evolved. Thus Ca. Methanoperedens was expected to catalyse bioelectrochemical CH4 oxidation in the Control-BES. But compared with the BEMR, a more significant drop (from 29.8% to 18.0%) of Ca. Methanoperedens population was observed, which was perhaps related to more sluggish EET kinetic in the Control-BES (Fig. 3).
In the current study, the current output was achieved in a BES (Control-BES) catalysed by an ANME-dominated methanotrophic consortium (Fig. 3b). Microbial community analysis indicated that ANME in the consortium likely implement essential physiological processes independently, including CH4 activation and EET to electrode (Fig. 5). However, the current output performance achieved in the Control-BES is extremely poor, which could be mainly attributed to two possible rate-limiting aspects: (i) electron production from CH4 that is limited by low CH4 bioavailability due to low solubility of CH4 in the solution and (ii) electron transfer from ANME to electrode constrained by poor EET ability of ANME.
For the first aspect, it was proposed to be overcome by an engineering approach in terms of CH4 delivery by HFMs [21, 32]. However, the principle cannot be directly applied into BESs, since commercially available HFMs that are made of nonconductive polymer cannot electrochemically interact with the anode to transfer electrons to outside circuit of BESs. In this study, this issue was addressed by amending soluble mediator of ferricyanide in the BEMR. Ferricyanide accepts electrons extracellularly, which means only EET-capable microbes catalyse ferricyanide reduction. The genetic information of ANME reveals that it encodes numerous multihaeme c-type cytochromes , indicating its EET capability. Actually, a similar ANME species (also belonging to the genus of Ca. Methanoperedens) has been proved to be able to reduce other iron species (i.e. ferric citrate, ferrihydrite) , which further consolidate the EET physiology of ANME in the inoculum. As no other CH4-oxidizing microbes appeared in the enriched methanotrophic culture (Fig. 5), the immediate onset of ferricyanide reduction being correlated to CH4 oxidation in batch incubations suggested ANME in the methanotrophic consortium is able to reduce ferricyanide (Fig. 1). These results demonstrated that ferricyanide can be used as an effective mediator to electrochemically bridge the HFMs (and the biofilm within) and the electrode. As shown in Fig. 6, methanotrophic biofilms colonized on the outer surface of HFMs oxidized CH4 diffused from inside of hollow fibres with soluble ferricyanide in the solution as the electron acceptor. Ferricyanide capturing electron from CH4 oxidation was reduced to ferrocyanide, which can be diffused to physically separated electrode surface and be oxidized to ferricyanide by the polarized electrode. The dynamic redox cycles of ferri/ferrocyanide enabled electrochemical bridging between HFMs (i.e. the site of CH4 oxidation) to the electrode (i.e. the site of final electron transfer), achieving sustainable bioelectrochemical CH4 oxidation on HFMs. This can be evidenced by the dependence of current on ferricyanide concentration (Additional file 1: Fig. S3). When ferricyanide was removed from the anode, the current dropped to a level matching that of the Control-BES, which revealed the indispensable role of the mediator on bridging electron transfer from the methanotrophic biofilm on HFMs to anode. The CH4 bioavailability limitation was overcome with the shuttling role of ferricyanide, as current output performance was accordingly enhanced with pressure increase and it almost doubled when the pressure was increased from 0 to 1.6 bar (Fig. 3).
Regarding the second limitation aspect, electron transfer from ANME to electrode could be constrained by poor EET ability of ANME. Microbial community analysis indicated that ANME likely activated CH4 and transferred electrons to the electrode independently (Fig. 5). However, the low current output in the Control-BES (Fig. 3) indicated that ANME seemed to be a weak electricigen in comparison to strong counterparts such as Geobacter . Use of mediators is suggested as an effective method to improve the EET kinetics of weak electricigen , which has been intensively confirmed to enhance performance of BESs with different strains as biocatalysts [34, 35]. This is proved to be applicable to ANME as well in the current study. When ferricyanide was applied as exogenous mediator in our current study, bioelectrochemical CH4 oxidation performance in the BEMR was enhanced dramatically by 126 times compared to that in the Control-BES when both were operated with ambient CH4 pressure (Fig. 3). Taken together, soluble mediator of ferricyanide enhances bioelectrochemical CH4 oxidation in the BEMR not only through directly facilitating EET of ANME, but also through indirectly increasing CH4 bioavailability by enabling application of HFMs for CH4 delivery. With HFMs pressurized (at 1.6 bar) to delivery CH4 substrate, the BEMR outperformed the Control-BES for approximately 244 times at CH4-based current output.
To date, multiple strategies have been reported to effectively enhance performance of CH4-powered BESs. These strategies, including genetic manipulation to increase methyl-coenzyme M reductase (MCR) expression for faster CH4 activation , as well as system optimization of configurating gas-diffusion electrode (GDE) to increase CH4 bioavailability , have been demonstrated to enhance performance by orders of magnitude in comparison to their counterparts without any manipulation on biocatalysts or BES configuration (Additional file 1: Table S2). The engineering approach presented in this study also shows impressive performance enhancement over these unmanipulated systems, although the current density achieved here is still inferior to some manipulated CH4-powered BESs reported to date (Additional file 1: Table S2). However, it should be pointed out the aim of this study is to validate the feasibility of a newly raised engineering strategy, while the strategy itself has not been optimized to its best conditions. The highest performance of CH4-powered BESs reported to date was indeed achieved by further system optimization on the basis of a convinced biological manipulation [13, 36]. Hence, system optimization is anticipated to further enhance the performance of bioelectrochemical CH4 oxidation based on the engineering strategy in the current study. One optimization aspect is to identify the optimal mediator species and concentration to obtain the faster mediated interaction between ANME and electrodes. Another optimization aspect is to further amplify the surface area of HFMs. Compared to the strategy of using GDE to increase CH4 bioavailability , application of HFMs features the availability of higher membrane pack intensity for the higher surface to volume ratio, which could potentially enable incredible CH4 diffusion and biomass incorporation as biofilm on HFMs.
Meanwhile, different methanotrophic consortia with different EET pathways were identified in different studies, which could also be associated with performance variations in different CH4-powdered BESs. Specifically, CH4 activation is all proposed to be activated by reverse methanogenesis via MCR in different methanotrophic consortia, while EET strategy varied depending on the MCR hosts (Additional file 1: Table S2). An intermediate-dependent EET mechanism has been proposed in different methanotrophic consortia [13, 19], within which CH4 activator interact with other electroactive bacteria (i.e. Geobacter) by diffusible intermediates, and final electron exchange with electrode are performed by the strong electricigen of Geobacter rather than CH4 activators themselves. The higher electroactivity of Geobacter enables more efficient electrical interaction between methanotrophic consortia and electrodes , which could also be the reason for the superior AOM performance in BESs identified with the intermediate-dependent EET mechanism [13, 19]. Nevertheless, compared with the direct interaction between methanotrophs and electrode in our study, the diffusion-dependent EET strategy in methanotrophic consortia is perhaps vulnerable to intermediate loss and environmental chemical fluctuations , which can affect energy efficiency for CH4 transformation. Hence, a more comprehensive investigation is required to know the importance of physiology in terms of EET strategy in CH4-based BESs. Together, our study provides an engineering strategy to enhance CH4 oxidation towards CH4-based bioelectrochemical technologies, and it also inspires us with architecture design for CH4-based BESs from both engineering and biocatalytic consortia perspectives. Due to the high redox potential of ferricyanide, although it is suitable for application in electrode-poised BESs for upgrading CH4 to chemicals or fuels, it is less favourable when compared to other mediators for application in a fuel cell mode for direct electricity generation. Further studies are warranted to reveal different fit-for-purpose mediators in different CH4-fuelled BESs. By utilizing suitable mediators, practical application of CH4 transformation for direct electricity generation or for fuel and chemicals production could be promoted with enhanced performance.
This study reports the application of redox mediator ferricyanide in a bioelectrochemical membrane reactor (BEMR) to enhance the catalysis of methane oxidation by an ANME-dominated methanotrophic consortium using an electrode as electron acceptor. On the one hand, the diffusible mediator bridges the distancing EET from methanotrophic biofilm on the non-conductive hollow fibres to physically separated anode, ultimately enabling membrane-based CH4 delivery for enhanced CH4 bioavailability. On the other hand, the mediator enhances EET kinetics of ANME directly by introducing the mediator-directed EET strategy. With hollow fibres pressurized at 1.6 bar to deliver CH4, the BEMR amended with mediator outperformed the Control-BES for approximately 244 times at CH4-based current output. Hence, this work provides an engineering strategy to enhance bioelectrochemical CH4 oxidation with ANME as the biocatalyst.
Batch incubations to test AOM coupled to ferricyanide reduction
Ferricyanide reduction theoretically can be coupled with CH4 oxidation to CO2, which can be described by the following equation . The Gibbs energy value is calculated based on standard conditions (pH 7, 298 K, 1 atm).
Before operating the bioelectrochemical systems described above to verify whether ferricyanide can be used as a soluble mediator to shuttle electrons between the HFMs-supported biofilm and the anode, we first assessed whether our methanotrophic culture was in fact able to catalyse ferricyanide reduction during CH4 oxidation, hence providing proof that the redox mediator was able to extract electrons from AOM.
Incubations were performed in 35 mL serum bottles (Sigma, USA). Methanotrophic biomass dominated by ANME archaea and anammox bacterium was used as the inoculum . Biomass collected from parent reactor was centrifuged at 6000 rpm for 15 min. The resulting biomass pellet was resuspended in fresh medium without nitrate (i.e. the basic medium used in the parent reactor, which composition was reported previously ). The procedure was repeated three times to eliminate any residual nitrate. Finally, the biomass was resuspended into triple volume of fresh medium (to yield a volatile suspended solids (VSS) concentration of ca. 0.25 g/L). K3[Fe(CN)6] was added into the culture resuspension at a concentration of 1 mM. The biomass was then distributed into 20 mL aliquots to each serum bottle, after which the bottles were sealed with butyl rubber stoppers and aluminium crimp caps. 10 mL 13CH4 (> 99.99% 13C; Sigma-Aldrich) was injected into the headspace each vail to provide the electron donor. All these operations were processed inside an anaerobic chamber (Coy Laboratory Products Inc., USA). Incubations were performed in triplicates, at room temperature (24 ± 1 °C), and in the dark. 1.0-mL liquid samples were routinely taken from the flasks and analysed to determine K3[Fe(CN)6] reduction and 13CO2 accumulation (see analytical methods below). 0.5 mL of liquid samples were filtrated for K3[Fe(CN)6] and K4[Fe(CN)6] quantification immediately; another 0.5 mL of liquid samples were taken and injected into 3 mL Exetainer vials (Labco, UK) for 13CO2 measurement.
BEMR configuration and operation
The schematic diagram of BEMR configuration and operation are illustrated in Fig. 7. It was constructed on the basis of a H-type electrochemical reactor configuration, which was assembled by using two commercially available glass bottles (Wenoote, China), each somewhat modified to accommodate various sampling ports and two flanges to allow clamping the bottles together. A piece of cation-exchange membrane (CMI-700, Membrane International Inc., USA) was cut into the size fitting the connection flange. It was then sandwiched between two pieces of pierced rubber gaskets, which was assembled between two bottles to separate them into two chambers (anode and cathode chamber). Each chamber had a total (i.e. empty volume) volume of 210 mL, and the working volume (i.e. liquid volume) was 160 mL. The top port of each bottle was sealed by a rubber stopper (Cole-Parmer, USA), kept in place by a plastic screw cap. Provision of CH4 to the anode chamber was achieved using a bundle of HFMs consisting of 300 pieces of hollow fibres (TM 830Y K200 nonporous polypropylene hollow fibre, Teijin Fibers, Ltd., Japan) each with length of 20 cm and an inner/outer fibre diameter of 50/200 μm. The HFM bundle was folded from the middle, then the two ends were jointed as such that all membrane sections were aligned. The end the HFM bundle was packed into a tubing fitting and then cemented together with epoxy glue. The fitting was pierced through the stopper, leaving a protruded part for tubing connection. Above assembly resulted into an effective membrane surface area (under anodic electrolyte) of 301.4 cm2. The end of the HFM was connected to a CH4 gas cylinder through a gas-impermeable hose. CH4 was fed to the anodic chamber by pressurizing the lumen of the HFM using a regulator, thus CH4 was forced to penetrate through the wall of the HFM. Next to the HFM bundle, a graphite rod (6.0 mm in diameter, Graphite Sales Inc., US) pierced through the rubber stopper with 7 cm immersed into the solution, was used as the anode electrode. In the cathode chamber, a piece of stainless steel fibre felt (5 cm × 2.5 cm × 1 mm, Lier Filter Ltd, Henan, China) was used as cathode electrode. External electrical continuity was guaranteed by connecting the cathode a titanium wire, which was then pierced through the rubber stopper. To ensure gas-tightness of each chamber, epoxy glue was applied onto every holes on the rubber stoppers where electrodes or fittings were installed.
The BEMR was operated in two stages: Stage 1 for the methanotrophic biofilm formation on HFMs (days 0–147) and Stage 2 for the tests of bioelectrochemical CH4 oxidation (days 147–255). During Stage 1, nitrate was used as the electron acceptor and the reactor was operated according to a similar procedure as used for the parent reactor, which has been shown as an effective method for the faster growth of methanotrophic biofilm on HFMs [39,40,41]. To inoculate the reactor, 100 mL biomass was seeded to the anodic chamber of BEMR, yielding an initial VSS concentration of ca. 0.75 g L−1. The growing medium consisted of 3 g L−1 KH2PO4, 6 g L−1 Na2HPO4, 0.15 g L−1 NH4Cl, 0.015 g L−1 CaCl2·2H2O, 0.1 g L−1 MgCl2·6H2O, NaHCO3 1.5 g L−1 and trace element . Nitrate and ammonium were introduced to the reactor by manual injection of their stock solutions (2.8 mol/L NO3−-N and 3.4 mol/L NH4+-N, respectively), to give initial concentrations between 14.0 to 15.0 mmol N/L for the two chemicals. Samples were taken regularly to monitor nitrate and ammonium consumption, and both nutrients were topped-up to their initial concentrations once their levels dropped below 4.0 mmol N/L. Nitrogen removal rates within each nutrient feeding pulse were determined as the slope of the concentration profiles of nitrate and ammonium. CH4 was continuously supplied to the reactor by applying 1.5 bar of pressure to the lumen of the HFM. The successful colonization of biofilm was determined by a gradual stabilization of nitrate removal rate.
During Stage 2, the cathodic chamber was filled with electrolyte containing 50 mM of phosphate buffer solution. Nitrate and ammonium additions were discontinued, the BEMR was connected to a potentiostat (CHI 1030C, CH Instruments Inc, USA), and, under the hypothesis that the anode would act as the final electron acceptor instead of nitrate, the anode electrode was polarized at 0.5 V versus an Ag/AgCl reference electrode in 3 M KCl (MF-2053, Basi, USA, + 0.210 V vs the standard hydrogen electrode, SHE). Potassium ferricyanide (K3[Fe(CN)6]) was added into the anodic chamber at a concentration of 1 mM to enable electron transfer between the biofilm grown onto the HFMs and the anode electrode. CH4 supply to the anode chamber was again obtained by pressurizing the HFM with CH4 gas. Different pressures (i.e. 0, 0.8, 1.2, 1.6 bar) were applied during this stage to investigate the correlation between CH4 availability and current output.
To provide control measurements, an identical of bioelectrochemical system (Control-BES) was set up and was operated under the same conditions to the BEMR, however, without HFMs assembly and amendment of ferricyanide as the redox mediator. CH4 in the Control-BES was fed directly to the headspace of the anodic chamber. Performance of CH4 oxidation was compared between the BEMR and the Control-BES by monitoring their current output as well as CO2 accumulation from AOM in the reactors throughout the operational course. As strong PBS was used in the anodic electrolyte, all of the CO2 produced from AOM was trapped in the solution. To measure CO2 accumulation in the solution, 0.5 mL of liquid samples was collected from the anode chamber and injected into 3 mL Exetainer vials (Labco, UK) for dissolved CO2 quantification.
To further verify the role of ferricyanide in bridging electron transfer from methanotrophic biofilm on HFMs to physically separated anode, a control experiment was performed by comparing chronoamperometric current in the presence and absence of ferricyanide. In the ferricyanide-amended BEMR with stable current output under the condition of pressurizing HFMs at 0.8 bar, ferricyanide was removed from the anolyte by continuous medium change in order not to upset biofilm on the HFMs. Specifically, as show in Additional file 1: Fig. S1, a feeding bottle was connected to the anode chamber, and 1 L of deoxygenated and CH4-saturated medium was pumped through the anode chamber into an overflow bottle at a flow rate of 5 mL/min to gradually wash ferricyanide away. A 1 L gas bag filled with CH4 was connected to the headspace of the feeding bottle to avoid vacuum during pumping. Liquid from the anode chamber was sampled every 15–30 min to quantify the variation of ferricyanide concentration over time. After the ferricyanide was removed from the anode chamber, a second stage of the test was performed by change the medium in the feeding bottle to 1 L of deoxygenated and CH4-saturated medium containing 1 mM ferricyanide to add it back to the anode chamber. Chronoamperometric current response to ferricyanide removal and addition was recorded at a poised potential of + 0.5 V Ag/AgCl to show the correlation between ferricyanide concentration and current output. Both BEMR and Control-BES were operated in batch mode, with internal mixing obtained by using magnetic bar and a magnetic stirrer set at 200 rpm. Both systems were operated at ambient temperature of 24 ± 1 °C.
Bioelectro-catalytic oxidation of CH4 in the BEMR and Control-BES was determined by a chronoamperometry (CA). The current versus time traces resulting from the application of a fixed anodic potential of 0.5 V were automatically constructed by the potentiostat with a resolution of 100 s per sample.
In addition, cyclic voltammetry (CV) was performed on the anodes of both of BEMR and Control-BES at the end of the experimentation using a multichannel potentiostat (Potentiostat/Galvanostat VMP3, Biologic Science Instrument, France). CVs traces were recorded between a potential window of − 0.7/ + 0.6 V vs Ag/AgCl at the scan rate 1.0 mV s−1.
Liquid samples were taken from batch incubations and BEMR using sterile syringes and needles and were immediately filtered through 0.22 μm disposable sterile Millipore filter (Merck). The concentrations of nitrogenous compounds (including NH4+, NO3−) were measured with a Lachat QuickChem 8000 flow injection analyzer (Lachat Instrument, Milwaukee, WI). K3[Fe(CN)6] and K4[Fe(CN)6] were quantified by a spectrophotometric method as detailed in Lai et al. . For the CO2 measurement, the samples stored in Exetainer vials were acidified with 0.2 mL anaerobic HCl solution (1 mM) and kept for at least 1 h before GC/MS measurement to allow CO2 to equilibrate with the headspace. Quantity and isotopic composition of CO2 in the headspace of Exetainer vials was measured by GC/MS (GC 7890A coupled to MSD 5975C; Agilent) as details described in Additional file 1.
Field-emission scanning electron microscope (FESEM)
FESEM images were obtained using a JOEL-7100F Field Emission Scanning Electron Microscopy. Details of sample preparation are provided in the Supporting Information.
16S rRNA gene sequencing
16S rRNA gene sequencing was applied to characterize microbial community dynamics. Total cell DNA was extracted using FastDNA SPIN for Soil kit (MP Biomedicals, USA) according to the manufacturer’s instructions. The extracted DNA concentration was quantified with NanoDrop 2000 (Thermo Fisher Scientific, USA). The 16S rRNA gene was amplified using the universal primer set 926F (5′-AAACTYAAAKGAATTGACGG-3′) and 1392R (5′-ACG-GGCGGTGTGTRC-3′). A QIAquick PCR Purification Kit (Qiagen) and a Quant-iT dsDNA HS assay kit (Invitrogen) were employed to purify and quantify the PCR products, respectively. Amplicons were pooled in equimolar concentration and sequenced with an Illumina sequencer (Illumina, USA). Raw sequencing data were quality-filtered and demultiplexed using Trimmomatic, with poor-quality sequences trimmed and removed. Subsequently, high-quality sequences at 97% similarity were clustered into operational taxonomic units (OTUs) using QIIME with default parameters, and representative OTU sequences were taxonomically aligned against Greengenes 16S rRNA database.
Calculation of coulombic efficiency
The coulombic efficiency (CE), defined as the fraction of electrons recovered from CH4 oxidation, was calculated as:
where I is the current output observed in BESs, e is the moles of electrons from each mole of CH4 oxidation (8 for CH4 from the half reaction: CH4 + 2H2O → CO2 + 8H+ + 8e−), n is the detected CO2 production (mol), F is Faraday’s constant (96,485 C mol−1).
Availability of data and materials
All data generated or analysed during this study are included in this manuscript and its Additional file 1.
Anaerobic methanotrophic archaea
Anaerobic oxidation of methane
Bioelectrochemical membrane reactor
Extracellular electron transfer
Hollow fibre membranes
Methyl-coenzyme M reductase
Solid fuel cells
Volatile suspended solids
Ge X, Yang L, Sheets JP, Yu Z, Li Y. Biological conversion of methane to liquid fuels: status and opportunities. Biotechnol Adv. 2014;32(8):1460–75.
Kalyuzhnaya M, Yang S, Rozova O, Smalley N, Clubb J, Lamb A, Gowda GN, Raftery D, Fu Y, Bringel F. Highly efficient methane biocatalysis revealed in a methanotrophic bacterium. Nat Commun. 2013;4(1):1–7.
Allen G. Biogeochemistry: Rebalancing the global methane budget. Nature. 2016;538(7623):46–8.
Holmes D, Smith J. Biologically produced methane as a renewable energy source. Advances in applied microbiology, vol. 97. New York: Elsevier; 2016. p. 1–61.
Ren ZJ. Microbial fuel cells: running on gas. Nat Energy. 2017;2(6):1–2.
Haynes CA, Gonzalez R. Rethinking biological activation of methane and conversion to liquid fuels. Nat Chem Biol. 2014;10(5):331.
Gür TM. Comprehensive review of methane conversion in solid oxide fuel cells: prospects for efficient electricity generation from natural gas. Prog Energy Combust Sci. 2016;54:1–64.
Chen Y, deGlee B, Tang Y, Wang Z, Zhao B, Wei Y, Zhang L, Yoo S, Pei K, Kim JH. A robust fuel cell operated on nearly dry methane at 500 C enabled by synergistic thermal catalysis and electrocatalysis. Nat Energy. 2018;3(12):1042–50.
Lawton TJ, Rosenzweig AC. Biocatalysts for methane conversion: big progress on breaking a small substrate. Curr Opin Chem Biol. 2016;35:142–9.
Conrado RJ, Gonzalez R. Envisioning the bioconversion of methane to liquid fuels. Science. 2014;343(6171):621–3.
Du Z, Li H, Gu T. A state of the art review on microbial fuel cells: a promising technology for wastewater treatment and bioenergy. Biotechnol Adv. 2007;25(5):464–82.
Wagner M. Microbiology: conductive consortia. Nature. 2015;526(7574):513–4.
McAnulty MJ, Poosarla VG, Kim K-Y, Jasso-Chávez R, Logan BE, Wood TK. Electricity from methane by reversing methanogenesis. Nat Commun. 2017;8(1):1–8.
McGlynn SE, Chadwick GL, Kempes CP, Orphan VJ. Single cell activity reveals direct electron transfer in methanotrophic consortia. Nature. 2015;526(7574):531–5.
Scheller S, Yu H, Chadwick GL, McGlynn SE, Orphan VJ. Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction. Science. 2016;351(6274):703–7.
Ettwig KF, Zhu B, Speth D, Keltjens JT, Jetten MS, Kartal B. Archaea catalyze iron-dependent anaerobic oxidation of methane. Proc Natl Acad Sci. 2016;113(45):12792–6.
Cai C, Leu AO, Xie G-J, Guo J, Feng Y, Zhao J-X, Tyson GW, Yuan Z, Hu S. A methanotrophic archaeon couples anaerobic oxidation of methane to Fe (III) reduction. ISME J. 2018;2018:1.
Ding J, Lu Y-Z, Fu L, Ding Z-W, Mu Y, Cheng SH, Zeng RJ. Decoupling of DAMO archaea from DAMO bacteria in a methane-driven microbial fuel cell. Water Res. 2017;110:112–9.
Yu L, Yang Z, He Q, Zeng RJ, Bai Y, Zhou S. Novel gas diffusion cloth bioanodes for high-performance methane-powered microbial fuel cells. Environ Sci Technol. 2018;53(1):530–8.
Xie X, Criddle C, Cui Y. Design and fabrication of bioelectrodes for microbial bioelectrochemical systems. Energy Environ Sci. 2015;8(12):3418–41.
Cai C, Hu S, Guo J, Shi Y, Xie G-J, Yuan Z. Nitrate reduction by denitrifying anaerobic methane oxidizing microorganisms can reach a practically useful rate. Water Res. 2015;87:211–7.
Xie G-J, Cai C, Hu S, Yuan Z. Complete nitrogen removal from synthetic anaerobic sludge digestion liquor through integrating anammox and denitrifying anaerobic methane oxidation in a membrane biofilm reactor. Environ Sci Technol. 2016;51(2):819–27.
Haroon MF, Hu S, Shi Y, Imelfort M, Keller J, Hugenholtz P, Yuan Z, Tyson GW. Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage. Nature. 2013;500(7464):567–70.
Cai C, Leu AO, Xie G-J, Guo J, Feng Y, Zhao J-X, Tyson GW, Yuan Z, Hu S. A methanotrophic archaeon couples anaerobic oxidation of methane to Fe (III) reduction. ISME J. 2018;12(8):1929–39.
Lai B, Yu S, Bernhardt PV, Rabaey K, Virdis B, Krömer JO. Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system. Biotechnol Biofuels. 2016;9(1):39.
Kracke F, Vassilev I, Krömer JO. Microbial electron transport and energy conservation–the foundation for optimizing bioelectrochemical systems. Front Microbiol. 2015;6:575.
Randviir EP. A cross examination of electron transfer rate constants for carbon screen-printed electrodes using electrochemical impedance spectroscopy and cyclic voltammetry. Electrochim Acta. 2018;286:179–86.
Chen H, Zhao L, Hu S, Yuan Z, Guo J. High-rate production of short-chain fatty acids from methane in a mixed-culture membrane biofilm reactor. Environ Sci Technol Lett. 2018;5(11):662–7.
Cai C, Hu S, Chen X, Ni B-J, Pu J, Yuan Z. Effect of methane partial pressure on the performance of a membrane biofilm reactor coupling methane-dependent denitrification and anammox. Sci Total Environ. 2018;639:278–85.
Zhang X, Xia J, Pu J, Cai C, Tyson GW, Yuan Z, Hu S. Biochar-mediated anaerobic oxidation of methane. Environ Sci Technol. 2019;53(12):6660–8.
Cai C, Shi Y, Guo J, Tyson GW, Hu S, Yuan Z. Acetate production from anaerobic oxidation of methane via intracellular storage compounds. Environ Sci Technol. 2019;53(13):7371–9.
Chen H, Luo J, Liu S, Yuan Z, Guo J. Microbial methane conversion to short-chain fatty acids using various electron acceptors in membrane biofilm reactors. Environ Sci Technol. 2019;53(21):12846–55.
Doyle LE, Marsili E. Weak electricigens: a new avenue for bioelectrochemical research. Biores Technol. 2018;258:354–64.
Martinez CM, Alvarez LH. Application of redox mediators in bioelectrochemical systems. Biotechnol Adv. 2018;36(5):1412–23.
Heydorn RLEC, Krull R, Dohnt K. Strategies for the targeted improvement of anodic electron transfer in microbial fuel cells. ChemBioEng Rev. 2020;1(7):4–17.
Yamasaki R, Maeda T, Wood TK. Electron carriers increase electricity production in methane microbial fuel cells that reverse methanogenesis. Biotechnol Biofuels. 2018;11(1):211.
Logan BE. Microbial fuel cells. New York: Wiley; 2008.
Hu S, Zeng RJ, Burow LC, Lant P, Keller J, Yuan Z. Enrichment of denitrifying anaerobic methane oxidizing microorganisms. Environ Microbiol Rep. 2009;1(5):377–84.
Luo J-H, Chen H, Hu S, Cai C, Yuan Z, Guo J. Microbial selenate reduction driven by a denitrifying anaerobic methane oxidation biofilm. Environ Sci Technol. 2018;52(7):4006–122.
Luo J-H, Wu M, Yuan Z, Guo J. Biological bromate reduction driven by methane in a membrane biofilm reactor. Environ Sci Technol Lett. 2017;4(12):562–6.
Luo J-H, Wu M, Liu J, Qian G, Yuan Z, Guo J. Microbial chromate reduction coupled with anaerobic oxidation of methane in a membrane biofilm reactor. Environ Int. 2019;130:104926.
We are grateful to the AWMC Analytical Services Laboratory (ASL) for all chemical analyses.
This work is supported by the Australian Research Council (ARC) through the projects of the Australian Laureate Fellowship (FL170100086) and Discovery Project (DP170104038).
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Zhang, X., Rabiee, H., Frank, J. et al. Enhancing methane oxidation in a bioelectrochemical membrane reactor using a soluble electron mediator. Biotechnol Biofuels 13, 173 (2020). https://doi.org/10.1186/s13068-020-01808-7
- Bioelectrochemical membrane reactor
- Redox mediator
- Bioelectrochemical methane oxidation