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Table 1 Composition of toxic compounds of the inhibitor cocktail

From: CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate

Inhibitor

1 × IC mixture (stock)

SCM 45% IC (used for screens)

Concentration (mM)

Concentration (g/L)

Concentration (mM)

Concentration (g/L)

Furfural

11.4

1.1

5.13

0.495

5-HMF

5.6

0.7

2.52

0.315

Formaldehyde

5.8

0.2

2.61

0.09

Acetic acid

45.6

2.7

20.52

1.215

Formic acid

15.5

0.7

6.975

0.315

Vanillin

1.6

0.2

0.72

0.09

Coniferyl aldehyde

1.6

0.03

0.72

0.0135

p-Hydroxybenzaldehyde

1.6

0.2

0.72

0.09

  1. Compound concentrations are listed in mM and g/L for IC mixtures of the 1 × stock and the mixture used for genetic screens
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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