Table 6 Benefits and shortcomings associated with methods used for S/G analysis reported in this work
From: Comparison of methodologies used to determine aromatic lignin unit ratios in lignocellulosic biomass
Method | Benefit | Shortcoming | |
|---|---|---|---|
Thioacidolysis | Small sample size, high reproducibility, wealth of historical data | Potential bias for aromatic lignin units released by β-O-4 linkages not being representative (although can be advantage for specificity), sensitive to other components in biomass impacting reaction, laborious sample preparation | |
Py-MBMS | Small sample size, rapid analysis, high reproducibility, multiple cell wall phenotype measurements possible | Destructive, requires comparison within species, potential bias for aromatic lignin units released by thermally labile linkages, overlap of some lignin-derived ions with non-lignin-derived species, semi-quantitative | |
1H–13C HSQC | Representative of whole cell wall, multiple cell wall phenotype measurements possible particularly including lignin linkage information | Semi-quantitative, large sample size requirement, long analysis time (which can be overcome if cryoprobe is available) | |
ssNMR Manders | Non-destructive | Underestimates contribution from S-lignin units | |
ssNMR Deconvolution | Non-destructive, representative of whole cell wall, multiple cell wall phenotype measurements possible | Low throughput (can be overcome if DNP hardware is available), sensitive to incorrect initial peak-fitting parameters, not appropriate for grass species, need other a priori data | |