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Table 1 Saccharide composition of onion epidermal wall by met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

  µg/mg dry weight %
Trial 1 Trial 2 Trial 3 Mean Total
mTFA Sae mTFA Sae mTFA Sae mTFA Sae
Glc 6.9 21.6 13.1 144.9 17.1 186.3 12.3 (2.3) 117.6 (19.8) 130.0 24
GalA 183.5 16.5 255.5 8.5 321.3 29.0 253.4 (23.1) 18.0 (2.4) 271.5 51
Gal 64.4 1.9 52.0 2.8 87.0 4.5 67.8 (7.2) 3.0 (0.6) 70.9 13
Xyl/Man* 27.8 6.7 21.7 7.6 29.5 8.8 26.3 (1.8) 7.7 (0.4) 34.0 6
Rha 14.8 0.5 5.4 1.0 12.9 1.0 11.0 (1.9) 0.8 (0.1) 11.9 2
Ara 8.0 0.2 5.1 1.0 11.1 0.8 8.1 (1.2) 0.6 (0.2) 8.7 2
Fuc 3.1 0.3 2.4 1.5 4.1 1.6 3.2 (0.4) 1.1 (0.4) 4.4 1
GlcA 0.8 0.0 0.0 0.0 1.8 0.0 0.9 (0.3) 0.0 (0.0) 0.9 0.2
Total 309.3 47.7 355.1 167.3 485.0 232.0 383.1 149.0 532.1 100
  1. mTFA, peel hydrolyzed with methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman’s hydrolysis) on residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates