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Table 3 Saccharide composition of onion epidermal wall pre-digested with Driselase enzyme followed by met/TFA/Saeman’s hydrolysis

From: Saccharide analysis of onion outer epidermal walls

  µg/mg dry weight %
Trial 1 Trial 2 Trial 3 Mean Total
Dri + mTFA Sae Dri + mTFA Sae Dri + mTFA Sae Dri + mTFA Sae
Glc 42.1 41.2 45.51 47.03 42.1 62.2 43.2 (1.0) 50.1 (5.1) 93.3 16.3
GalA 395.8 0.0 320.19 0.00 308.7 6.8 341.6 (22.3) 2.3 (1.9) 343.8 59.9
Gal 110.0 3.2 40.69 3.27 66.3 4.3 72.3 (16.5) 3.6 (0.3) 75.9 13.2
Xyl/Man* 20.9 4.4 18.57 3.63 19.8 13.1 19.8 (0.5) 7.1 (2.5) 26.8 4.7
Rha 21.7 0.4 9.67 0.43 11.3 0.8 14.2 (3.1) 0.6 (0.1) 14.8 2.6
Ara 21.9 0.6 6.79 0.71 9.5 1.6 12.7 (3.8) 1.0 (0.3) 13.7 2.4
Fuc 2.1 0.5 4.2 0.41 2.0 2.4 2.8 (0.6) 1.1 (0.5) 3.9 0.7
GlcA 3.6 0.0 0.0 0.0 1.6 0.0 1.7 (0.8) 0.0 (0.0) 1.7 0.3
Total 618.1 50.2 445.6 55.5 461.3 91.3 508.3 65.7 574.0 100
  1. Dri + mTFA, peel Driselase digested and the soluble sugars hydrolyzed by methanolysis with TFA; Sae, 2-step sulfuric acid (Saeman's hydrolysis) on Driselase digested residue; Xyl/Man*, HPAEC-PAD parameters did not separate xylose and mannose peaks. µg/mg values were adjusted using correction factors listed in “Materialsandmethods”. (SEM), standard error of mean of 3 biological replicates