Table 3 Saccharide composition of onion epidermal wall pre-digested with Driselase enzyme followed by met/TFA/Saeman’s hydrolysis
µg/mg dry weight | % | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Trial 1 | Trial 2 | Trial 3 | Mean | Total | ||||||
Dri + mTFA | Sae | Dri + mTFA | Sae | Dri + mTFA | Sae | Dri + mTFA | Sae | |||
Glc | 42.1 | 41.2 | 45.51 | 47.03 | 42.1 | 62.2 | 43.2 (1.0) | 50.1 (5.1) | 93.3 | 16.3 |
GalA | 395.8 | 0.0 | 320.19 | 0.00 | 308.7 | 6.8 | 341.6 (22.3) | 2.3 (1.9) | 343.8 | 59.9 |
Gal | 110.0 | 3.2 | 40.69 | 3.27 | 66.3 | 4.3 | 72.3 (16.5) | 3.6 (0.3) | 75.9 | 13.2 |
Xyl/Man* | 20.9 | 4.4 | 18.57 | 3.63 | 19.8 | 13.1 | 19.8 (0.5) | 7.1 (2.5) | 26.8 | 4.7 |
Rha | 21.7 | 0.4 | 9.67 | 0.43 | 11.3 | 0.8 | 14.2 (3.1) | 0.6 (0.1) | 14.8 | 2.6 |
Ara | 21.9 | 0.6 | 6.79 | 0.71 | 9.5 | 1.6 | 12.7 (3.8) | 1.0 (0.3) | 13.7 | 2.4 |
Fuc | 2.1 | 0.5 | 4.2 | 0.41 | 2.0 | 2.4 | 2.8 (0.6) | 1.1 (0.5) | 3.9 | 0.7 |
GlcA | 3.6 | 0.0 | 0.0 | 0.0 | 1.6 | 0.0 | 1.7 (0.8) | 0.0 (0.0) | 1.7 | 0.3 |
Total | 618.1 | 50.2 | 445.6 | 55.5 | 461.3 | 91.3 | 508.3 | 65.7 | 574.0 | 100 |