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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris

Fig. 2

ApV1 phytase retains the thermostability improvement after deglycosylation although glycosylation contributes to overall stability. Remaining activity of glycosylated and deglycosylated AppA (a) and ApV1 (b) phytases. Phytase activity was determined by the p-NPP assay after incubation at 65, 75 or 85 °C. Remaining activity was calculated as a percentage of phytase activity without high-temperature treatment. Deglycosylated forms are indicated as “DG”. Data are represented as mean values ± standard deviation (n = 3)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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