Km (mg ml−1) | kcat (s−1) | kcat/Km \((\mathrm{ml }\,{\mathrm{mg}}^{-1}\, {\mathrm{ s}}^{-1})\) | Tm (°C) | |
---|---|---|---|---|
IaGal WT | 0.47 ± 0.08 | 34 ± 2 | 73 ± 13 | 105 |
Variant 1 | 0.38 ± 0.09 | 14 ± 4 | 36 ± 13 | 102 |
Variant 2 | 0.48 ± 0.08 | 16 ± 3 | 34 ± 9 | 101 |
Variant 3 | 2.3 ± 0.6 | 28 ± 15 | 12 ± 7 | 103 |
Variant 4 | 2.7 ± 0.3 | 20 ± 6 | 7 ± 2 | 103 |
BlGal | 0.94 ± 0.15 | 2.5·102 ± 0.1·102 | 2.6·102 ± 0.4·102 | 53 |
MtGal | 1.0 ± 0.4 | 3.6·102 ± 0.3·102 | 3.5·102 ± 1.3·102 | N/A |