Omics analysis coupled with gene editing revealed potential transporters and regulators related to levoglucosan metabolism efficiency of the engineered Escherichia coli

Chang, Dongdong; Wang, Cong; Ul Islam, Zia; Yu, Zhisheng

doi:10.1186/s13068-022-02102-4

Biotechnology for Biofuels and Bioproducts

Table 2 Down-regulated proteins at both early- and mid-log phases of levoglucosan utilization relative to fructose utilization

From: Omics analysis coupled with gene editing revealed potential transporters and regulators related to levoglucosan metabolism efficiency of the engineered Escherichia coli

Differentially expressed proteins (DEPs)	Accession No.	Gene name	Log₂ FC
Differentially expressed proteins (DEPs)	Accession No.	Gene name	Early log phase	Mid log phase
Energy production and conversion‑related proteins
FdrA family protein	A0A140NAI7	yahF	− 2.42	− 2.17
Aldehyde-alcohol dehydrogenase	A0A140NCE4	adhE	− 1.82	− 1.52
Molybdopterin dehydrogenase FAD-binding	A0A140N5N6	ygeT	− 3.02	− 1.07
Cytochrome o ubiquinol oxidase, subunit III	A0A140NC92	cyoC	− 2.03	− 1.29
Molybdopterin oxidoreductase Fe₄S₄ region	A0A140NE68	fdhF	− 1.20	− 2.82
Hydrogenase (NiFe) small subunit HydA	A0A140NDP4	hyaA	− 2.10	− 1.06
Nickel-dependent hydrogenase large subunit	A0A140NB83	hyaB	− 1.05	− 1.20
Amino acid transport and metabolism‑related proteins
Inner-membrane translocator	A0A140N716	livH	− 1.03	− 2.99
T-protein	A0A140N544	tyrA	− 1.66	− 2.29
Carbohydrate transport and metabolism‑related proteins
Fructose-bisphosphate aldolase	A0A140N821	fba	− 1.56	− 1.27
d-Erythrose-4-phosphate dehydrogenase	A0A140N827	epd	− 1.97	− 1.35
Ribulose-phosphate 3-epimerase	A0A140SS41	alsE	− 1.23	− 4.07
PTS system, fructose subfamily, IIC subunit	A0A140N8J0	fruA	− 3.24	− 4.43
Phosphofructokinase	A0A140N679	fruK	− 3.67	− 3.81
PTS system fructose-specific EIIA component	A0A140N9Z8	fruB	− 4.11	− 3.59
Replication, recombination and repair‑related proteins
Integration host factor subunit beta	A0A140NDV2	ihfB	− 1.51	− 1.22
Poorly characterized proteins
5′-Deoxynucleotidase YfbR	A0A140N9N4	yfbR	− 5.22	− 4.74
Protein ViaA	A0A140NFS7	viaA	− 1.25	− 1.12
ATPase RavA	A0A140NI88	ravA	− 1.43	− 1.18
Phage minor tail protein G	A0A140NE00	ECBD_2862	− 1.22	− 3.06
Protein YcfR	A0A140N4R7	ycfR	− 2.54	− 2.45
Type VI secretion system effector, Hcp1 family	A0A140N758	yhhZ	− 1.27	− 5.48

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