Table 1 Strains and plasmids used
| Â | Characteristics | Sources |
|---|---|---|
Strains | ||
 E. coli BL21 | Host for plasmid construction | Lab stock |
 Paecilomyces variotii FN89 | Biodetoxification fungus isolated by our lab | Lab stock |
 C. glutamicum S9114 | Industrial strain | SIIM |
 C. glutamicum ZW1 | C. glutamicum S9114 carrying pH36-ado-aar | This study |
 C. glutamicum ZW2 | C. glutamicum S9114 carrying pH36-ado-rbs-aar | This study |
 C. glutamicum ZW3 | C. glutamicum S9114 carrying pH36-ado-TacM-aar | This study |
 C. glutamicum HW4 | C. glutamicum S9114 deleting fasR and carrying aar and ado | This study |
 C. glutamicum HW4-pH36-aar | C. glutamicum HW4 carrying pH36-aar | This study |
 C. glutamicum HW4-pH36-ado | C. glutamicum HW4 carrying pH36-ado | This study |
 C. glutamicum HW4-pH36-pntAB | C. glutamicum HW4 carrying pH36-pntAB | This study |
 C. glutamicum HW4-pH36-udhA | C. glutamicum HW4 carrying pH36-udhA | This study |
 C. glutamicum HW4-pH36-sgdh | C. glutamicum HW4 carrying pH36-sgdh | This study |
 C. glutamicum HW4-pH36-aasS | C. glutamicum HW4 carrying pH36-aasS | This study |
 C. glutamicum HW4-pH36-fdh1 | C. glutamicum HW4 carrying pH36-fdh1 | This study |
 C. glutamicum S9114-pH36-oleTJE | C. glutamicum S9114 carrying pH36-oleTJE | This study |
 C. glutamicum S9114-pH36-oleTMC | C. glutamicum S9114 carrying pH36-oleTMC | This study |
 C. glutamicum S9114-pH36-NsoleTJE | C. glutamicum S9114 carrying pH36-NsoleTJE | This study |
 C. glutamicum S9114-pH36-RsoleTJE | C. glutamicum S9114 carrying pH36-RsoleTJE | This study |
 C. glutamicum S9114-pH36-NsoleTMC | C. glutamicum S9114 carrying pH36-NsoleTMC | This study |
 C. glutamicum S9114-pH36-RsoleTMC | C. glutamicum S9114 carrying pH36-RsoleTMC | This study |
 C. glutamicum S9114-pH36-NCgl1221-oleTMC | C. glutamicum S9114 carrying pH36-NCgl1221-oleTMC | This study |
 C. glutamicum S9114-pH36-NCgl1337-oleTMC | C. glutamicum S9114 carrying pH36-NCgl1337-oleTMC | This study |
 C. glutamicum S9114-pH36-PorB-oleTMC | C. glutamicum S9114 carrying pH36-porB-oleTMC | This study |
 C. glutamicum S9114-pH36-PorC-oleTMC | C. glutamicum S9114 carrying pH36-porC-oleTMC | This study |
 C. glutamicum S9114-pEftu-NCgl1221-oleTMC | C. glutamicum S9114 carrying pEftu-NCgl1221-oleTMC | This study |
 C. glutamicum S9114-pEftu-NCgl1337-oleTMC | C. glutamicum S9114 carrying pEftu-NCgl1337-oleTMC | This study |
 C. glutamicum S9114-pEftu-PorB-oleTMC | C. glutamicum S9114 carrying pEftu-porB-oleTMC | This study |
 C. glutamicum S9114-pEftu-PorC-oleTMC | C. glutamicum S9114 carrying pEftu-porC-oleTMC | This study |
 C. glutamicum HW4-pH36-oleTJE | C. glutamicum HW4 carrying pH36-oleTJE | This study |
 C. glutamicum HW4-pH36-oleTMC | C. glutamicum HW4 carrying pH36-oleTMC | This study |
 C. glutamicum HW4-pH36-NsoleTJE | C. glutamicum HW4 carrying pH36-NsoleTJE | This study |
 C. glutamicum HW4-pH36-RsoleTJE | C. glutamicum HW4 carrying pH36-RsoleTJE | This study |
 C. glutamicum HW4-pH36-NsoleTMC (C. glutamicum HW5) | C. glutamicum HW4 carrying pH36-NsoleTMC | This study |
 C. glutamicum HW4-pH36-RsoleTMC (C. glutamicum HW6) | C. glutamicum HW4 carrying pH36-RsoleTMC | This study |
Plasmids | ||
 pK18mobsacB | Mobilizable vector in C. glutamicum, kanamycin resistance, sacB | Wang et al. [17] |
 pH36mob | Overexpression vector, kanamycin resistance | Lab stock |
 pEftumob | Insert promoter Peftu at the back of promoter Ptrc in pTRCmob | Lab stock |
 pH36-ado-aar | pH36mob carrying aar and ado | This study |
 pH36-ado-rbs-aar | pH36mob carrying aar, rbs and ado | This study |
 pH36-ado-TacM-aar | pH36mob carrying ado under H36 control and aar under TacM control | This study |
 pH36-aar | pH36mob carrying aar | This study |
 pH36-ado | pH36mob carrying ado | This study |
 pH36-pntAB | pH36mob carrying pntAB | This study |
 pH36-sgdh | pH36mob carrying sgdh | This study |
 pH36-udhA | pH36mob carrying udhA | This study |
 pH36-aasS | pH36mob carrying aasS | This study |
 pH36-fdh1 | pH36mob carrying fdh1 | This study |
 pH36-oleTJE | pH36mob carrying oleTJE | This study |
 pH36-oleTMC | pH36mob carrying oleTMC | This study |
 pH36-NsoleTJE | pH36mob carrying oleTJE with signal peptide of Ncgl1289 | This study |
 pH36-RsoleTJE | pH36mob carrying oleTJE with signal peptide of RS04950 | This study |
 pH36-NsoleTMC | pH36mob carrying oleTMC with signal peptide of Ncgl1289 | This study |
 pH36-RsoleTMC | pH36mob carrying oleTMC with signal peptide of RS04950 | This study |
 pH36-NCgl1221-oleTMC | pH36mob carrying oleTMC with signal peptide of Ncgl1221 | This study |
 pH36-NCgl1337-oleTMC | pH36mob carrying oleTMC with signal peptide of Ncgl1337 | This study |
 pH36-PorB-oleTMC | pH36mob carrying oleTMC with signal peptide of PorB | This study |
 pH36-PorC-oleTMC | pH36mob carrying oleTMC with signal peptide of PorC | This study |
 pEftu-NCgl1221-oleTMC | pH36-NCgl1221-oleTMC with H36 promoter replaced by Eftu promoter | This study |
 pEftu-NCgl1337-oleTMC | pH36-NCgl1337-oleTMC with H36 promoter replaced by Eftu promoter | This study |
 pEftu-PorB-oleTMC | pH36-PorB-oleTMC with H36 promoter replaced by Eftu promoter | This study |
 pEftu-PorC-oleTMC | pH36-PorC-oleTMC with H36 promoter replaced by Eftu promoter | This study |
 pK18-ΔfasR-aar-rbs-ado | Plasmid for fasR knockout in the genome and carrying aar, rbs and ado | This study |