Skip to main content
Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa

Fig. 5

Relative expression levels of genes induced by L-tryptophan and the IAA yield of modified strain SC2-M1. Relative expression levels of genes gatA1, patA, ilvB3, fusE, sdr2, and nit2 in the logarithmic phase of strain SC2-M1 in the test group (named as M1-T) that was cultured with 3 mM L-tryptophan compared with the control group (named as M1) without L-tryptophan (A). The IAA yield of strain SC2-M1 overexpressing genes patA, ilvB3, and fusE (B). The IAA yields were tested by the colorimetric method and adding 3 mM L-tryptophan in Landy medium for 72 h at 25 °C. SC2-M1-P is the control strain containing empty plasmid pHY300PLK, Strains M1-patA, M1-ilvB, and M1-fusE represent genes patA, ilvB3, and fusE overexpressing strains, respectively. Strain M1-C1 represents patA-ilvB3-fusE co-expressing strain. Compared with the control, data that significantly increased (p < 0.05) were marked with *

Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us