Table 2 Strains and plasmids used in this study
Strains or plasmids | Genotype/properties | Source/references |
|---|---|---|
Strain | ||
 E. coli DH5α | F-φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk −, mk +) supE44λ- thi-1 gyrA96 relA1 phoA | TransGen Biotech |
 CQDH5α-Qiu | E. coli DH5α derivative; {pHY300PLK-gfp-cm-ter} | [60] |
 CQDH5α-promotera | E. coli DH5α derivative; {pHY300PLK-Promoter-gfp-cm-ter} | This work |
 CQDH5α-amyb | E. coli DH5α derivative; {pHY300PLK-P04420/P04420-4/P04420-6/P04420-8/P04420-9-α-amylase-ter} | This work |
 DH5α-patA | DH5α derivative; {pHY300PLK-P04420-patA} | This work |
 DH5α-ilvB | DH5α derivative; {pHY300PLK-P04420-ilvB3} | This work |
 DH5α-fusE | DH5α derivative; {pHY300PLK-P04420-fusE} | This work |
 DH5α-C | DH5α derivative; {pHY300PLK-P04420-patA-ilvB3-fusE} | This work |
 DH5α-Pgap-IAM | DH5α derivative; {pHY300PLK-Pgap-iaam} | This work |
 DH5α-P04420-IAM | DH5α derivative; {pHY300PLK-P04420-iaam} | This work |
 DH5α-Pgap-IPyAAKA | DH5α derivative; {pHY300PLK-Pgap-aro8-kdc-aldH} | This work |
 DH5α-P04420-IPyAAKA | DH5α derivative; {pHY300PLK-P04420-aro8-kdc-aldH} | This work |
 DH5α-P04420-IPyAJP6 | DH5α derivative; {pHY300PLK-P04420-ELJP6_14505-ipdC-ELJP6_00725} | This work |
 B. subtilis 168 | trpC2 | BGSC, [60] |
 CQ168-Qiu | B. subtilis 168 derivative; {pHY300PLK-gfp-cm-ter} | [60] |
 CQ168-promoterc | B. subtilis 168 derivative; {pHY300PLK-Promoter-gfp-cm-ter} | This work |
 P. polymyxa SC2 | Wild type, isolated from the rhizosphere of pepper plants in Guizhou, China | [66] |
 P. polymyxa SC2-M1 | Spontaneous mutant of P. polymyxa SC2 | [68] |
 CQM1-Qiu | P. polymyxa SC2-M1 derivative; {pHY300PLK-gfp-cm-ter} | [60] |
 CQM1-Promoterd | P. polymyxa SC2-M1 derivative; {pHY300PLK-Promoter-gfp-cm-ter} | This work |
 CQM1-amye | P. polymyxa SC2-M1 derivative; {pHY300PLK-P04420/P04420-4/P04420-6/P04420-8/P04420-9-α-amylase-ter} | This work |
 SC2-M1-P | SC2-M1derivative; {pHY300PLK} | This work |
 M1-patA | SC2-M1derivative; {pHY300PLK-P04420-patA} | This work |
 M1-ilvB | SC2-M1derivative; {pHY300PLK-P04420-ilvB3} | This work |
 M1-fusE | SC2-M1derivative; {pHY300PLK-P04420-fusE} | This work |
 M1-C1 | SC2-M1 derivative; {pHY300PLK-P04420-patA–ilvB3–fusE} | This work |
 M1-Pgap-IAM | SC2-M1 derivative; {pHY300PLK-Pgap-iaam} | This work |
 M1-P04420-IAM | SC2-M1 derivative; {pHY300PLK-P04420-iaam} | This work |
 M1-Pgap-IPyAAKA | SC2-M1 derivative; {pHY300PLK-Pgap-aro8-kdc-aldH} | This work |
 M1-P04420-IPyAAKA | SC2-M1 derivative; {pHY300PLK-P04420-aro8-kdc-aldH} | This work |
 M1-P04420-IPyAJP6 | SC2-M1 derivative; {pHY300PLK-P04420-ELJP6_14505-ipdC-ELJP6_00725} | This work |
Plasmid | ||
 pHY300PLK | E. coli and B. subtilis shuttle vector; Ampr, Tetr | TaKaRa |
 pHY300PLK-gfp-cm | pHY300PLK-gfp-cm-ter | [60] |
 pHY300PLK-Promoterf-gfp-cm | pHY300PLK-Promoter-gfp-cm-ter | This work |
 pHY300PLK-Promoterg-amylase | pHY300PLK-P04420/P04420-4/P04420-6/P04420-8/P04420-9-α-amylase-ter | This work |
 pHY300PLK-Pgap | pHY300PLK-Pgap | [60] |
 pHY300PLK-P04420 | pHY300PLK-P04420 | This work |
 pHY300PLK-patA | pHY300PLK-P04420-patA | This work |
 pHY300PLK-ilvB3 | pHY300PLK-P04420-ilvB3 | This work |
 pHY300PLK-fusE | pHY300PLK-P04420-fusE | This work |
 pHY300PLK-patA-ilvB3-fusE | pHY300PLKK-P04420-patA-ilvB3-fusE | This work |
 pHY300PLK-Pgap-IAM | pHY300PLKK-Pgap-iaam | This work |
 pHY300PLK-P04420-IAM | pHY300PLK-P04420-iaam | This work |
 pHY300PLK-Pgap-IPyAAKA | pHY300PLK-Pgap-aro8-kdc-aldH | This work |
 pHY300PLK-P04420-IPyAAKA | pHY300PLK-P04420-aro8-kdc-aldH | This work |
 pHY300PLK-P04420-IPyAJP6 | pHY300PLK-P04420-ELJP6_14505-ipdC-ELJP6_00725 | This work |