Table 1 Strains and plasmids used in this study
From: Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation
Strains and plasmids | Description | Reference or source |
|---|---|---|
Saccharomyces cerevisiae strain | ||
CEN.PK 530-1C | MATa tpi1(41–707)::loxP-KanMX4-loxP | Overkamp et al. [34] |
B184M | UV-mutated strain derived from CEN.PK 530.1C | Huang et al. [7] |
B0 | B184M/CPOTud | This study |
BG01 | B184M/pCP-G01 | This study |
BG02 | B184M/pCP-G02 | This study |
BG03 | B184M/pCP-G03 | This study |
BG04 | B184M/pCP-G04 | This study |
BG05 | B184M/pCP-G05 | This study |
BG06 | B184M/pCP-G06 | This study |
BG07 | B184M/pCP-G07 | This study |
BH01 | B184M/pCP-H01 | This study |
BH02 | B184M/pCP-H02 | This study |
BH03 | B184M/pCP-H03 | This study |
BH04 | B184M/pCP-H04 | This study |
BH05 | B184M/pCP-H05 | This study |
Plasmid | ||
pUC57-Mini_G | Vector pUC57-Mini with colG gene (colG pre-peptide‐colG pro-peptide‐colG chain) from Clostridium histolyticum | GenScript co. Ltd |
pUC57-Mini_H | Vector pUC57-Mini with colH gene (colH pre-peptide‐colH pro-peptide‐colH chain) from Clostridium histolyticum | GenScript co. Ltd |
CPOTud | 2 μm, AmpR, TPI1p-(gene of interest)-TPI1t, POT1 gene from S. pombe as a selection marker | Liu et al. [40] |
pAlphaAmyCPOT | CPOTud‐(α-factor leader + spacer‐amylase gene) | Liu et al. [40] |
pCP_G01 | CPOTud‐(α-factor leader + spacer‐colG pro-peptide‐colG chain) | This study |
pCP_G02 | CPOTud‐(α-factor leader‐colG pro-peptide‐colG chain) | This study |
pCP_G03 | CPOTud‐(α-factor pre‐colG pro-peptide‐colG chain) | This study |
pCP_G04 | CPOTud‐(α-factor leader + spacer‐colG chain) | This study |
pCP_G05 | CPOTud‐(α-factor leader-colG chain) | This study |
pCP_G06 | CPOTud‐(α-factor pre‐colG chain) | This study |
pCP_G07 | CPOTud‐(colG pre-peptide‐colG pro-peptide‐colG Chain) | This study |
pCP_H01 | CPOTud‐(α-factor leader + spacer‐colH pro-peptide‐colH chain) | This study |
pCP_H02 | CPOTud‐(α-factor leader‐colH pro-peptide‐colH chain) | This study |
pCP_H03 | CPOTud‐(α-factor leader + spacer-colH chain) | This study |
pCP_H04 | CPOTud‐(α-factor leader‐colH chain) | This study |
pCP_H05 | CPOTud‐(colH pre-peptide‐colH pro-peptide‐colH chain) | This study |