From: Progress and perspectives of enzymatic preparation of human milk fat substitutes
Lipase | Reaction type/substrates | Solvent | Effect | Ref. |
---|---|---|---|---|
Candida sp. 99–125 lipase | Esterification between glycerol and OA | Acetone; tetrahydrofuran (THF); t-butanol; 4-methyl-2-pentanone; chloroform; toluene; tetrachloromethane; cyclohexane; n-hexane; n-heptane; n-octane | Solvent with high log P: high activity, weak sn-1,3 selectivity | [90] |
Novozym 435 | Esterification between glycerol and OA | Acetone; THF; t-butanol; 4-methyl-2-pentanone; chloroform; toluene; tetrachloromethane; cyclohexane; n-hexane; n-heptane; n-octane | Solvent with high log P: high activity, weak sn-1,3 selectivity | [94] |
Whole-cell lipase from Aspergillus niger GZUF36 | Glycerolysis between triolein (OOO) and glycerol | Acetonitrile; t-butanol; n-hexane; n-heptane; n-octane; THF; trichloromethane | Relatively hydrophobic solvent: high activity (except for acetonitrile) | [92] |
Lipozyme TL IM | Esterification between monoolein and OA | t-Butanol; t-amyl alcohol; toluene; hexamethylene; n-hexane | Solvent with high log P: high activity and acyl migration, weak sn-1,3 selectivity decreased | [91] |
Novozym 435 | Ethanolysis of the fungal oil | Solventless; hexane; dichloromethane; acetone; t-butanol; ethanol | Solventless system: the highest acyl migration rate; polar solvent system: low acyl migration rate | [93] |
Lipozyme RM IM | Acidolysis between tripalmitin and FFAs | Acetone; t-butanol; n-hexane | Polar solvent: low acyl migration | [26] |
Lipozyme TL IM | Esterification between monoolein and OA | Acetone; t-butanol; trichloromethane; n-hexane | Solvent with high log P: high activity, weak sn-1,3 selectivity | [95] |