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Table 2 Kinetic parameters of TmXdhA for cellooligosaccharides and xylooligosaccharides

From: Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93

Substrate

KM (mM)

kcat (s−1)

kcat/KM (s−1∙mM−1)

Cellobiose

0.36 ± 0.04

16.7 ± 0.7

46.2 ± 5.8

Cellotriose

0.43 ± 0.04

17.6 ± 0.6

40.1 ± 4.2

Cellotetraose

0.53 ± 0.04

23.8 ± 1.1

45.1 ± 4.0

Xylobiose

0.16 ± 0.01

17.5 ± 0.4

108.9 ± 9.5

Xylotriose

0.11 ± 0.01

22.3 ± 0.4

200.9 ± 14.5

Xylotetraose

0.13 ± 0.01

25.0 ± 0.5

187.7 ± 15.2

  1. Standard deviation is calculated from three replicate reactions
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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