Fig. 2
From: Engineering isoprenoids production in metabolically versatile microbial host Pseudomonas putida
Engineering heterologous pathway for isoprenol production in P. putida. A Configuration of P. putida strains with the engineered isoprenol pathway plasmids. B–D Production results by the engineered P. putida strains from 1% glucose. B Cell growth. The initial OD600 of 0 h was set at 0.05. C Isoprenol production. Isoprenol levels of different strains were not detectable at 0 h; D Glucose consumption. Error bars indicate one standard deviation of triplicates. NudB, dihydroneopterin triphosphate diphosphatase (E. coli); AtoB, acetoacetyl-CoA synthase (E. coli); HMGS, HMG-CoA synthase; HMGR, HMG-CoA reductase; MvaS, HMG-CoA synthase; MvaE, HMG-CoA reductase; MK, mevalonate kinase; PMK, phosphomevalonate kinase; PMD, phosphomevalonate decarboxylase. The footnote of enzymes indicates their sources: Sc, S. cerevisiae; Sa, Staphylococcus aureus; Ef, Enterococcus faecalis. BBR1, broad host range replication origin; KanR, kanamycin-resistant antibiotic marker