Fig. 4
From: Spatial–temporal regulation of fatty alcohol biosynthesis in yeast
Dynamical regulation of fatty alcohol biosynthesis under control of fatty acid/acyl-CoA responsive promoters. A Fatty alcohol titer of strains with FAR pathway controlled by fatty acid/acyl-CoA responsive promoters and PGAL1,10. B Characterization of fatty acid/acyl-CoA responsive promoters by eGFP fluorescence intensity. eGFP controlled by various promoters, including PAAD6, PADH6, PBDH2, PSTE3, and PGAL1, 10, were integrated in control strain GN33, generating strains GN33-AAD6, GN33-ADH6, GN33-BDH2, GN33-STE3, and GN33-GAL1,10, respectively. Engineered strains were cultivated in minimal medium with 20 g/L glucose, and eGFP fluorescence was measured with different time to evaluate their specific responses. Data are presented as mean ± SD of three biologically independent yeast clones with displayed data points. Red asterisks indicate statistical significance as determined using paired t test (n.s. p > 0.05; *P < 0.05; ** < 0.01)