Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000;24:45–66.
Article
CAS
Google Scholar
Ahmad M, Hirz M, Pichler H, Schwab H. Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol. 2014;98:5301–17.
Article
CAS
Google Scholar
Goncalves AM, Pedro AQ, Maia C, Sousa F, Queiroz JA, Passarinha LA. Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins. J Microbiol Biotechnol. 2013;23:587–601.
Article
CAS
Google Scholar
Huang J, Xia J, Yang Z, Guan F, Cui D, Guan G, Jiang W, Li Y. Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel. Biotechnol Biofuels. 2014;7:111.
Article
Google Scholar
Lunsdorf H, Gurramkonda C, Adnan A, Khanna N, Rinas U. Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. Microb Cell Fact. 2011;10:48.
Article
Google Scholar
Navone L, Vogl T, Luangthongkam P, Blinco JA, Luna-Flores CH, Chen X, von Hellens J, Mahler S, Speight R. Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris. Biotechnol Biofuels. 2021;14:80.
Article
CAS
Google Scholar
Nylen A, Chen MT. Production of full-length antibody by Pichia pastoris. Methods Mol Biol. 2018;1674:37–48.
Article
CAS
Google Scholar
Xu H, Shi M, Shao C, Li H, Wu J, Yu Y, Fang F, Guo Y, Xiao W. Development of IL-15/IL-15Ralpha sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives. Microb Cell Fact. 2021;20:115.
Article
CAS
Google Scholar
Bevis BJ, Hammond AT, Reinke CA, Glick BS. De novo formation of transitional ER sites and Golgi structures in Pichia pastoris. Nat Cell Biol. 2002;4:750–6.
Article
CAS
Google Scholar
Mogelsvang S, Gomez-Ospina N, Soderholm J, Glick BS, Staehelin LA. Tomographic evidence for continuous turnover of Golgi cisternae in Pichia pastoris. Mol Biol Cell. 2003;14:2277–91.
Article
CAS
Google Scholar
Rossanese OW, Soderholm J, Bevis BJ, Sears IB, O’Connor J, Williamson EK, Glick BS. Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae. J Cell Biol. 1999;145:69–81.
Article
CAS
Google Scholar
Karbalaei M, Rezaee SA, Farsiani H. Pichia pastoris: a highly successful expression system for optimal synthesis of heterologous proteins. J Cell Physiol. 2020;235:5867–81.
Article
CAS
Google Scholar
Bolhuis A, Matzen A, Hyyrylainen HL, Kontinen VP, Meima R, Chapuis J, Venema G, Bron S, Freudl R, van Dijl JM. Signal peptide peptidase- and ClpP-like proteins of Bacillus subtilis required for efficient translocation and processing of secretory proteins. J Biol Chem. 1999;274:24585–92.
Article
CAS
Google Scholar
Owji H, Nezafat N, Negahdaripour M, Hajiebrahimi A, Ghasemi Y. A comprehensive review of signal peptides: structure, roles, and applications. Eur J Cell Biol. 2018;97:422–41.
Article
CAS
Google Scholar
Aw R, McKay PF, Shattock RJ, Polizzi KM. A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization. Protein Expr Purif. 2018;149:43–50.
Article
CAS
Google Scholar
Julius D, Blair L, Brake A, Sprague G, Thorner J. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase. Cell. 1983;32:839–52.
Article
CAS
Google Scholar
Brake AJ, Merryweather JP, Coit DG, Heberlein UA, Masiarz FR, Mullenbach GT, Urdea MS, Valenzuela P, Barr PJ. Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci USA. 1984;81:4642–6.
Article
CAS
Google Scholar
Barrero JJ, Casler JC, Valero F, Ferrer P, Glick BS. An improved secretion signal enhances the secretion of model proteins from Pichia pastoris. Microb Cell Fact. 2018;17:161.
Article
CAS
Google Scholar
Yang L, Dong W, He J, Ren X, Yan W. Expression and purification of natural N-terminal recombinant bovine pancreatic trypsin inhibitor from Pichia pastoris. Biol Pharm Bull. 2008;31:1680–5.
Article
CAS
Google Scholar
Liang S, Li C, Ye Y, Lin Y. Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris. Biotechnol Lett. 2013;35:97–105.
Article
CAS
Google Scholar
Duan G, Ding L, Wei D, Zhou H, Chu J, Zhang S, Qian J. Screening endogenous signal peptides and protein folding factors to promote the secretory expression of heterologous proteins in Pichia pastoris. J Biotechnol. 2019;306:193–202.
Article
CAS
Google Scholar
De Schutter K, Lin YC, Tiels P, Van Hecke A, Glinka S, Weber-Lehmann J, Rouze P, Van de Peer Y, Callewaert N. Genome sequence of the recombinant protein production host Pichia pastoris. Nat Biotechnol. 2009;27:561–6.
Article
Google Scholar
Huang Y, Lin T, Lu L, Cai F, Lin J, Jiang YE, Lin Y. Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris. Microb Cell Fact. 2021;20:209.
Article
CAS
Google Scholar
Jumper J, Evans R, Pritzel A, Green T, Figurnov M, Ronneberger O, Tunyasuvunakool K, Bates R, Zidek A, Potapenko A, et al. Highly accurate protein structure prediction with AlphaFold. Nature. 2021;596:583–9.
Article
CAS
Google Scholar
Baghban R, Farajnia S, Rajabibazl M, Ghasemi Y, Mafi A, Hoseinpoor R, Rahbarnia L, Aria M. Yeast expression systems: overview and recent advances. Mol Biotechnol. 2019;61:365–84.
Article
CAS
Google Scholar
Chahal S, Wei P, Moua P, Park SP, Kwon J, Patel A, Vu AT, Catolico JA, Tsai YF, Shaheen N, et al. Structural characterization of the alpha-mating factor prepro-peptide for secretion of recombinant proteins in Pichia pastoris. Gene. 2017;598:50–62.
Article
CAS
Google Scholar
Aggarwal S, Mishra S. Differential role of segments of alpha-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulating factor emerging from a wild type or codon optimized copy of the gene. Microb Cell Fact. 2020;19:199.
Article
CAS
Google Scholar
Lin-Cereghino GP, Stark CM, Kim D, Chang J, Shaheen N, Poerwanto H, Agari K, Moua P, Low LK, Tran N, et al. The effect of alpha-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris. Gene. 2013;519:311–7.
Article
CAS
Google Scholar
Vadhana AK, Samuel P, Berin RM, Krishna J, Kamatchi K, Meenakshisundaram S. Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris. Enzyme Microb Technol. 2013;52:177–83.
Article
CAS
Google Scholar
Latiffi AA, Salleh AB, Rahman RN, Oslan SN, Basri M. Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and alpha-factor secretion signal in Pichia pastoris. Genes Genet Syst. 2013;88:85–91.
Article
CAS
Google Scholar
Massahi A, Calik P. Endogenous signal peptides in recombinant protein production by Pichia pastoris: from in-silico analysis to fermentation. J Theor Biol. 2016;408:22–33.
Article
CAS
Google Scholar
Fitzgerald I, Glick BS. Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting. Microb Cell Fact. 2014;13:125.
Article
Google Scholar
Huang Y, Long Y, Li S, Lin T, Wu J, Zhang Y, Lin Y. Investigation on the processing and improving the cleavage efficiency of furin cleavage sites in Pichia pastoris. Microb Cell Fact. 2018;17:172.
Article
CAS
Google Scholar