Fig. 2

B. subtilis grown in anaerobic BES with glucose as substrate and K₃[Fe(CN)₆] acting as a mediator. Current density and charge logged at poised anode potential 0.7 V and abiotic electrochemical characterisation of the mediator via CV using graphite felt as the working electrode a. Optical density and pH b, glucose, lactate and 2,3-butanediol concentrations c were measured from the reactors with applied potential [AP] and open circuit [OC]. Results are based on average data of three biological replicates and standard deviations are represented as coloured area and error bars