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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Integrated microbiology and metabolomic analysis reveal the improvement of rice straw silage quality by inoculation of Lactobacillus brevis

Fig. 4

Metabolite analysis of rice straw silage with various strain inoculations. a Principal Component Analysis (PCA) of metabolite profiles in rice straw silage with different strain inoculations. The 95% confidence ellipses are shown in respective colors for distinct communities. Dashed circles in blue and red signify groups inoculated without Lac33 and with Lac33, respectively. b Relative abundance of metabolite categories in silage groups with and without L. brevis R33 inoculation. Metabolites classified into different groups and labeled accordingly. c Volcano plots illustrate metabolites up or down-regulated between silage groups without and with L. brevis R33 inoculation. Dashed lines represent fold change (FC > 2 and < -2) and P-value (P < 0.05) thresholds. d Analysis of up and downregulated metabolites summarized and displayed at the subclass level. Abbreviations: CK, no inoculation; LB, inoculated with LB; Leu, inoculated with L. pseudomesenteroides; Lac17, inoculated with L. buchneri R17; Leu + Lac17, inoculated with L. pseudomesenteroides and L. buchneri R17; Lac33, inoculated with L. brevis R33; Leu + Lac33, inoculated with L. pseudomesenteroides and L. brevis R33; Lac17 + 33, inoculated with L. buchneri R17 and L. brevis R33; Leu + Lac17 + 33, inoculated with L. pseudomesenteroides, L. buchneri R17, and L. brevis R33

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