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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: Photoautotrophic cultivation of a Chlamydomonas reinhardtii mutant with zeaxanthin as the sole xanthophyll

Fig. 1

Organization of photosynthetic complexes of Wt and the Δzl mutant. a Sucrose density gradient fractionation of Wt and Δzl solubilized with 0.6% dodecyl‐α‐D‐maltoside (α‐DM). The composition of the green bands is indicated on the left. b Distribution as percentages of the chlorophylls in the gradient fractions. c Carotenoid distribution in the different gradient fractions. d Thylakoid pigment–protein complexes separated by non-denaturing Deriphat PAGE. e Western blotting images used for immunotitration of thylakoid proteins using antibodies against PsaA, CP43, CP26, and LHCII. Chlorophylls (2, 1, 0.5, and 0.25 µg) were loaded on cellulose membrane. f Immunotitration of thylakoid proteins. Data were corrected by CP43 and normalized to the Wt ratio. All the experiment was performed in biological replicates (n = 3)

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