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  1. Chemical and physical pretreatment of lignocellulosic biomass improves substrate reactivity for increased microbial biofuel production, but also restricts growth via the release of furan aldehydes, such as fur...

    Authors: Sonya M Clarkson, Scott D Hamilton-Brehm, Richard J Giannone, Nancy L Engle, Timothy J Tschaplinski, Robert L Hettich and James G Elkins
    Citation: Biotechnology for Biofuels 2014 7:165
  2. Engineering of Saccharomyces cerevisiae for the simultaneous utilization of hexose and pentose sugars is vital for cost-efficient cellulosic bioethanol production. This yeast lacks specific pentose transporters a...

    Authors: Jeroen G Nijland, Hyun Yong Shin, René M de Jong, Paul P de Waal, Paul Klaassen and Arnold JM Driessen
    Citation: Biotechnology for Biofuels 2014 7:168
  3. A key focus in sustainable biofuel research is to develop cost-effective and energy-saving approaches to increase saccharification of lignocellulosic biomass. Numerous efforts have been made to identify critic...

    Authors: Jijiao Zeng, Deepak Singh, Difeng Gao and Shulin Chen
    Citation: Biotechnology for Biofuels 2014 7:161
  4. For economical bioethanol production from lignocellulosic materials, the major technical challenges to lower the production cost are as follows: (1) The microorganism should use efficiently all glucose and xyl...

    Authors: Ke-Ke Cheng, Jing Wu, Zhang-Nan Lin and Jian-An Zhang
    Citation: Biotechnology for Biofuels 2014 7:166
  5. Clostridium pasteurianum is one of the most promising biofuel producers within the genus Clostridium owing to its unique metabolic ability to ferment glycerol into butanol. Although an efficient means is availabl...

    Authors: Michael E Pyne, Murray Moo-Young, Duane A Chung and C Perry Chou
    Citation: Biotechnology for Biofuels 2014 7:163
  6. Studies in bioconversions have continuously sought the development of processing strategies to overcome the “close physical association” between plant cell wall polymers thought to significantly contribute to ...

    Authors: Michael J Selig, Lisbeth G Thygesen and Claus Felby
    Citation: Biotechnology for Biofuels 2014 7:159
  7. Agricultural residue is more efficient than purified cellulose at inducing lignocellulolytic enzyme production in Penicillium oxalicum GZ-2, but in Trichoderma reesei RUT-C30, cellulose induces a more efficient r...

    Authors: Hanpeng Liao, Shuixian Li, Zhong Wei, Qirong Shen and Yangchun Xu
    Citation: Biotechnology for Biofuels 2014 7:162
  8. Among themophilic consolidated bioprocessing (CBP) candidate organisms, environmental isolates of Clostridium clariflavum have demonstrated the ability to grow on xylan, and the genome of C. clariflavum DSM 19732...

    Authors: Javier A Izquierdo, Sivakumar Pattathil, Anna Guseva, Michael G Hahn and Lee R Lynd
    Citation: Biotechnology for Biofuels 2014 7:136
  9. Microbial lipids produced from lignocellulosic biomass hold great promise for the biodiesel industry. These lipids usually consist of three major processes: pretreatment, enzymatic hydrolysis and lipid product...

    Authors: Zhiwei Gong, Hongwei Shen, Xiaobing Yang, Qian Wang, Haibo Xie and Zongbao K Zhao
    Citation: Biotechnology for Biofuels 2014 7:158
  10. The utilization of biomass from microalgae for biofuel production is one of the key elements for the development of a sustainable and secure energy supply. Among the different microalgae, Chlorella species are of...

    Authors: Stefano Cazzaniga, Luca Dall'Osto, Joanna Szaub, Luca Scibilia, Matteo Ballottari, Saul Purton and Roberto Bassi
    Citation: Biotechnology for Biofuels 2014 7:157
  11. Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concen...

    Authors: Evert K Holwerda, Philip G Thorne, Daniel G Olson, Daniel Amador-Noguez, Nancy L Engle, Timothy J Tschaplinski, Johannes P van Dijken and Lee R Lynd
    Citation: Biotechnology for Biofuels 2014 7:155
  12. Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral ...

    Authors: Jian Ye, Jing Qu, Hui-Zhu Mao, Zhi-Gang Ma, Nur E Rahman, Chao Bai, Wen Chen, Shu-Ye Jiang, Srinivasan Ramachandran and Nam-Hai Chua
    Citation: Biotechnology for Biofuels 2014 7:149
  13. The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel...

    Authors: Jesse B Therien, Oleg A Zadvornyy, Matthew C Posewitz, Donald A Bryant and John W Peters
    Citation: Biotechnology for Biofuels 2014 7:154
  14. Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial ...

    Authors: Camilla Lambertz, Megan Garvey, Johannes Klinger, Dirk Heesel, Holger Klose, Rainer Fischer and Ulrich Commandeur
    Citation: Biotechnology for Biofuels 2014 7:135
  15. Yarrowia lipolytica is an oleaginous yeast capable of metabolizing glucose to lipids, which then accumulate intracellularly. However, it lacks the suite of cellulolytic enzymes required to break down biomass cell...

    Authors: Hui Wei, Wei Wang, Markus Alahuhta, Todd Vander Wall, John O Baker, Larry E Taylor, Stephen R Decker, Michael E Himmel and Min Zhang
    Citation: Biotechnology for Biofuels 2014 7:148
  16. Sugar cane internodes can be divided diagonally into four fractions, of which the two innermost ones are the least recalcitrant pith and the moderately accessible pith-rind interface. These fractions differ in...

    Authors: Anikó Várnai, Thales HF Costa, Craig B Faulds, Adriane MF Milagres, Matti Siika-aho and André Ferraz
    Citation: Biotechnology for Biofuels 2014 7:153
  17. Third generation biodiesel processing from microbial lipids using low-cost lignocellulosic feedstocks has attracted much attention. Endophytes isolated from oleaginous plants possibly have the capacity to accu...

    Authors: Qin Zhang, Yanbin Li and Liming Xia
    Citation: Biotechnology for Biofuels 2014 7:152
  18. Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol producti...

    Authors: Qining Sun, Marcus Foston, Xianzhi Meng, Daisuke Sawada, Sai Venkatesh Pingali, Hugh M O’Neill, Hongjia Li, Charles E Wyman, Paul Langan, Art J Ragauskas and Rajeev Kumar
    Citation: Biotechnology for Biofuels 2014 7:150
  19. Biogas is a renewable energy carrier which is used for heat and power production or, in the form of purified methane, as a vehicle fuel. The formation of methane from organic materials is carried out by a mixe...

    Authors: Linn Solli, Othilde Elise Håvelsrud, Svein Jarle Horn and Anne Gunn Rike
    Citation: Biotechnology for Biofuels 2014 7:146
  20. Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds. In particular, Clos...

    Authors: Satyakam Dash, Thomas J Mueller, Keerthi P Venkataramanan, Eleftherios T Papoutsakis and Costas D Maranas
    Citation: Biotechnology for Biofuels 2014 7:144
  21. Mineral elements present in lignocellulosic biomass feedstocks may accumulate in biorefinery process streams and cause technological problems, or alternatively can be reaped for value addition. A better unders...

    Authors: Duy Michael Le, Hanne R Sørensen, Niels Ole Knudsen, Jan K Schjoerring and Anne S Meyer
    Citation: Biotechnology for Biofuels 2014 7:141
  22. A major obstacle, and perhaps the most important economic barrier to the effective use of plant biomass for the production of fuels, chemicals, and bioproducts, is our current lack of knowledge of how to effic...

    Authors: Daehwan Chung, Sivakumar Pattathil, Ajaya K Biswal, Michael G Hahn, Debra Mohnen and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:147
  23. Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide c...

    Authors: Hiroyuki Inoue, Stephen R Decker, Larry E Taylor, Shinichi Yano and Shigeki Sawayama
    Citation: Biotechnology for Biofuels 2014 7:151
  24. Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. D...

    Authors: Jenna Young, Daehwan Chung, Yannick J Bomble, Michael E Himmel and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:142
  25. For the development of lignocellulosic biofuels a common strategy to release hemicellulosic sugars and enhance the enzymatic digestibility of cellulose is the heat pretreatment of biomass with dilute acid. Dur...

    Authors: Ana B Ibáñez and Stefan Bauer
    Citation: Biotechnology for Biofuels 2014 7:145
  26. Enzymatic breakdown of lignocellulosic biomass is a known bottleneck for the production of high-value molecules and biofuels from renewable sources. Filamentous fungi are the predominant natural source of enzy...

    Authors: David Navarro, Marie-Noëlle Rosso, Mireille Haon, Caroline Olivé, Estelle Bonnin, Laurence Lesage-Meessen, Didier Chevret, Pedro M Coutinho, Bernard Henrissat and Jean-Guy Berrin
    Citation: Biotechnology for Biofuels 2014 7:143
  27. The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this a...

    Authors: Yubin Zheng, Xiaochen Yu, Tingting Li, Xiaochao Xiong and Shulin Chen
    Citation: Biotechnology for Biofuels 2014 7:125
  28. Lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. An understanding of the toxic effects ...

    Authors: Shihui Yang, Mary Ann Franden, Steven D Brown, Yat-Chen Chou, Philip T Pienkos and Min Zhang
    Citation: Biotechnology for Biofuels 2014 7:140
  29. Biohydrogen production through dark fermentation using organic waste as a substrate has gained increasing attention in recent years, mostly because of the economic advantages of coupling renewable, clean energ...

    Authors: Iulian Zoltan Boboescu, Mariana Ilie, Vasile Daniel Gherman, Ion Mirel, Bernadett Pap, Adina Negrea, Éva Kondorosi, Tibor Bíró and Gergely Maróti
    Citation: Biotechnology for Biofuels 2014 7:139
  30. The bioconversion of cellulose into simple sugars or chemicals has attracted extensive attention in recent decades. The crystal allomorphs of cellulose are key factor affecting cellulose saccharification. Howe...

    Authors: Ting Cui, Jihong Li, Zhipei Yan, Menghui Yu and Shizhong Li
    Citation: Biotechnology for Biofuels 2014 7:134
  31. Genetically customised Saccharomyces cerevisiae that can produce ethanol and additional bio-based chemicals from sustainable agro-industrial feedstocks (for example, residual plant biomass) are of major interest ...

    Authors: Claire M Hull, E Joel Loveridge, Nicola J Rolley, Iain S Donnison, Steven L Kelly and Diane E Kelly
    Citation: Biotechnology for Biofuels 2014 7:133
  32. Members of the thermophilic, anaerobic Gram-positive bacterial genus Caldicellulosiruptor grow optimally at 65 to 78°C and degrade lignocellulosic biomass without conventional pretreatment. Decomposition of compl...

    Authors: Joseph Groom, Daehwan Chung, Jenna Young and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:132
  33. Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulase...

    Authors: Thiago M Mello-de-Sousa, Rita Gorsche, Alice Rassinger, Marcio J Poças-Fonseca, Robert L Mach and Astrid R Mach-Aigner
    Citation: Biotechnology for Biofuels 2014 7:129
  34. The efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysa...

    Authors: Lei Ji, Jinshui Yang, Hua Fan, Yi Yang, Baozhen Li, Xuejian Yu, Ning Zhu and Hongli Yuan
    Citation: Biotechnology for Biofuels 2014 7:130
  35. Enzymatic hydrolysis is a crucial step of biomass conversion into biofuels and different pretreatments have been proposed to improve the process efficiency. Amongst the various factors affecting hydrolysis yie...

    Authors: Jefferson Esquina Tsuchida, Camila Alves Rezende, Rodrigo de Oliveira-Silva, Marisa Aparecida Lima, Marcel Nogueira d’Eurydice, Igor Polikarpov and Tito José Bonagamba
    Citation: Biotechnology for Biofuels 2014 7:127
  36. The expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement co...

    Authors: Mark D Harrison, Zhanying Zhang, Kylie Shand, Barrie Fong Chong, Jason Nichols, Paul Oeller, Ian M O'Hara, William OS Doherty and James L Dale
    Citation: Biotechnology for Biofuels 2014 7:131
  37. Efficient industrial processes for converting plant lignocellulosic materials into biofuels are a key to global efforts to come up with alternative energy sources to fossil fuels. Novel cellulolytic enzymes ha...

    Authors: Sebastian GA Konietzny, Phillip B Pope, Aaron Weimann and Alice C McHardy
    Citation: Biotechnology for Biofuels 2014 7:124
  38. The concept of adaptive evolution implies underlying genetic mutations conferring a selective advantage to an organism under particular environmental conditions. Thus, a flow cytometry-based strategy was used ...

    Authors: Natarajan Velmurugan, Minji Sung, Sung Sun Yim, Min S Park, Ji Won Yang and Ki Jun Jeong
    Citation: Biotechnology for Biofuels 2014 7:117
  39. The lipid content of microalgae is regarded as an important indicator for biodiesel. Many attempts have been made to increase the lipid content of microalgae through biochemical and genetic engineering. Signif...

    Authors: Jianhui Zhang, Qiang Hao, Lili Bai, Jin Xu, Weibo Yin, Liying Song, Ling Xu, Xuejie Guo, Chengming Fan, Yuhong Chen, Jue Ruan, Shanting Hao, Yuanguang Li, Richard R-C Wang and Zanmin Hu
    Citation: Biotechnology for Biofuels 2014 7:128
  40. Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. H...

    Authors: Yuping Lin, Kulika Chomvong, Ligia Acosta-Sampson, Raíssa Estrela, Jonathan M Galazka, Soo Rin Kim, Yong-Su Jin and Jamie HD Cate
    Citation: Biotechnology for Biofuels 2014 7:126
  41. Shewanella oneidensis MR-1 is one of the model microorganisms used for extracellular electron transfer. In this study, to elucidate the capability and the relevant metabolic processes of S. oneidensis MR-1 involv...

    Authors: Longfei Mao and Wynand S Verwoerd
    Citation: Biotechnology for Biofuels 2014 7:118
  42. The emergence of biofuels produced through yeast fermentation represents an important avenue for sustainable energy production. Despite all its advantages, this process is vulnerable to contamination by other ...

    Authors: Juliano Bertozzi Silva and Dominic Sauvageau
    Citation: Biotechnology for Biofuels 2014 7:123
  43. Efficient xylose fermentation by yeast would improve the economical and sustainable nature of biofuels production from lignocellulosic biomass. However, the efficiency of xylose fermentation by the yeast Saccharo...

    Authors: Sun-Mi Lee, Taylor Jellison and Hal S Alper
    Citation: Biotechnology for Biofuels 2014 7:122
  44. Wheat straw is an attractive substrate for second generation ethanol production because it will complement and augment wheat production rather than competing with food production. However, like other sources o...

    Authors: Samuel RA Collins, Nikolaus Wellner, Isabel Martinez Bordonado, Andrea L Harper, Charlotte N Miller, Ian Bancroft and Keith W Waldron
    Citation: Biotechnology for Biofuels 2014 7:121
  45. Lignocellulosic biomass from plant biomass, especially softwoods, are well-known to present difficulties during attempts at hydrolysis due to their rigid structure. Pretreatment of lignocellulosic biomass with...

    Authors: Kazuma Ogura, Kazuaki Ninomiya, Kenji Takahashi, Chiaki Ogino and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2014 7:120
  46. Hydrogen is regarded as an attractive future energy carrier for its high energy content and zero CO2 emission. Currently, the majority of hydrogen is generated from fossil fuels. However, from an environmental pe...

    Authors: Zhicheng Lai, Muzi Zhu, Xiaofeng Yang, Jufang Wang and Shuang Li
    Citation: Biotechnology for Biofuels 2014 7:119
  47. The recalcitrance of lignocellulosic biomass is a major limitation for its conversion into biofuels by enzymatic hydrolysis. The use of a pretreatment technology is an essential step to diminish biomass recalc...

    Authors: Shaoni Sun, Xuefei Cao, Shaolong Sun, Feng Xu, Xianliang Song, Run-Cang Sun and Gwynn Lloyd Jones
    Citation: Biotechnology for Biofuels 2014 7:116