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Correction to: Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover
Biotechnology for Biofuels volume 11, Article number: 327 (2018)
- The original article was published in Biotechnology for Biofuels 2018 11:316
Correction to: Biotechnol Biofuels (2018) 11:316 https://doi.org/10.1186/s13068-018-1315-5
Following publication of the original article , the authors wish to update the information under the heading “Enzyme adsorption comparison on lignin materials derived from acid and alkali pre-treated corn stover”. The corrected text is as follows:
Enzyme adsorption comparison on lignin materials derived from acid and alkali pre-treated corn stover.
To further understand the role of lignin materials in enzyme adsorption, we enzymatically hydrolyzed dilute acid pre-treated and dilute alkali pre-treated corn stover excessively to remove all the carbohydrates that are hydrolyzable by the enzyme cocktail applied. The obtained cellulolytic enzyme lignin (CEL) materials were used to investigate their enzyme adsorption kinetics (Fig. 3), and the Langmuir adsorption model was used to fit the data (Table 2). It was found that CEL isolated from dilute alkali pre-treated corn stover (CEL-alkali-CS) had a maximum enzyme adsorption capacity (Ebm) of 10.09 mg protein/g CEL, and the CEL from dilute acid pre-treated corn stover (CEL-acid-CS) had a much higher Ebm of 19.90 mg protein/g CEL. The association constant for CEL-alkali-CS and CEL-acid-CS was 4.2 mL/mg and 3.5 mL/mg, respectively. The distribution coefficient (Kp) was also calculated to characterize the interaction between enzyme and CEL. Kp of CEL-acid-CS was 69.64 mL/g, which was higher than 42.38 for CEL-alkali-CS, indicating that lignin isolated from dilute acid pre-treated corn stover had a higher enzyme adsorption capability than CEL-alkali-CS. Therefore, the enzyme adsorption difference in enzymatic hydrolysis of acid pre-treated CS and alkali pre-treated CS (Fig. 2) is also due to the adsorption property difference of lignin.
Yuan Y, Zhai R, Li Y, Chen X, Jin M. Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover. Biotechnol Biofuels. 2018;11:316. https://doi.org/10.1186/s13068-018-1315-5.
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Yuan, Y., Zhai, R., Li, Y. et al. Correction to: Developing fast enzyme recycling strategy through elucidating enzyme adsorption kinetics on alkali and acid pretreated corn stover. Biotechnol Biofuels 11, 327 (2018). https://doi.org/10.1186/s13068-018-1322-6