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  1. Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-d-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymeri...

    Authors: Mario Klimacek, Chao Zhong and Bernd Nidetzky
    Citation: Biotechnology for Biofuels 2021 14:134
    Content type: Research Published on:

  2. Microalgae-based high-density fuels offer an efficient and environmental pathway towards decarbonization of the transport sector and could be produced as part of a globally distributed network without competin...

    Authors: John Roles, Jennifer Yarnold, Karen Hussey and Ben Hankamer
    Citation: Biotechnology for Biofuels 2021 14:133
    Content type: Research Published on:

  3. Microalgae can contribute to more than 40% of global primary biomass production and are suitable candidates for various biotechnology applications such as food, feed products, drugs, fuels, and wastewater trea...

    Authors: Mingcan Wu, Ming Du, Guimei Wu, Feimiao Lu, Jing Li, Anping Lei, Hui Zhu, Zhangli Hu and Jiangxin Wang
    Citation: Biotechnology for Biofuels 2021 14:132
    Content type: Research Published on:

  4. Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification and make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active e...

    Authors: Monika Tõlgo, Silvia Hüttner, Peter Rugbjerg, Nguyen Thanh Thuy, Vu Nguyen Thanh, Johan Larsbrink and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2021 14:131
    Content type: Research Published on:

  5. Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the orga...

    Authors: Miriam Edel, Gunnar Sturm, Katrin Sturm-Richter, Michael Wagner, Julia Novion Ducassou, Yohann Couté, Harald Horn and Johannes Gescher
    Citation: Biotechnology for Biofuels 2021 14:130
    Content type: Research Published on:

  6. The overwhelming concerns due to over exploitation of fossil resources necessitate the utilization of alternative energy resources. Biodiesel has been considered as one of the most adaptable alternative to fos...

    Authors: Mustafa Kamal Pasha, Lingmei Dai, Dehua Liu, Miao Guo and Wei Du
    Citation: Biotechnology for Biofuels 2021 14:129
    Content type: Review Published on:

  7. Dye-decolorizing peroxidases (DyPs) represent a novel family of heme peroxidases that use H2O2 as the final electron acceptor to catalyze the oxidation of various organic compounds. A DyP from Irpex lacteus F17 (

    Authors: Liuqing Li, Tao Wang, Taohua Chen, Wenhan Huang, Yinliang Zhang, Rong Jia and Chao He
    Citation: Biotechnology for Biofuels 2021 14:128
    Content type: Research Published on:

  8. Nowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other bio-based products. The use of enzymes at several stages of the production pr...

    Authors: Lisanne Hameleers, Leena Penttinen, Martina Ikonen, Léa Jaillot, Régis Fauré, Nicolas Terrapon, Peter J. Deuss, Nina Hakulinen, Emma R. Master and Edita Jurak
    Citation: Biotechnology for Biofuels 2021 14:127
    Content type: Research Published on:

  9. β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal glycoside hydrolase family 3 (GH3) β-gluc...

    Authors: Mu-Rong Kao, Su-May Yu and Tuan-H ua David Ho
    Citation: Biotechnology for Biofuels 2021 14:126
    Content type: Research Published on:

  10. During the acetogenic step of anaerobic digestion, the products of acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis and methanogenesis are highly i...

    Authors: Anna Detman, Michał Bucha, Laura Treu, Aleksandra Chojnacka, Łukasz Pleśniak, Agnieszka Salamon, Ewa Łupikasza, Robert Gromadka, Jan Gawor, Agnieszka Gromadka, Wojciech Drzewicki, Marta Jakubiak, Marek Janiga, Irena Matyasik, Mieczysław K. Błaszczyk, Mariusz Orion Jędrysek…
    Citation: Biotechnology for Biofuels 2021 14:125
    Content type: Research Published on:

  11. Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation p...

    Authors: Jule Brandenburg, Johanna Blomqvist, Volha Shapaval, Achim Kohler, Sabine Sampels, Mats Sandgren and Volkmar Passoth
    Citation: Biotechnology for Biofuels 2021 14:124
    Content type: Research Published on:

  12. Sugarcane is one of the most crucial energy crops that produces high yields of sugar and lignocellulose. The cellulose crystallinity index (CrI) and lignin are the two kinds of key cell wall features that acco...

    Authors: Xinru Li, Fumin Ma, Chengping Liang, Maoyao Wang, Yan Zhang, Yufei Shen, Muhammad Adnan, Pan Lu, Muhammad Tahir Khan, Jiangfeng Huang and Muqing Zhang
    Citation: Biotechnology for Biofuels 2021 14:123
    Content type: Research Published on:

  13. Lytic polysaccharide monooxygenases (LPMOs) are attracting large attention due their ability to degrade recalcitrant polysaccharides in biomass conversion and to perform powerful redox chemistry.

    Authors: Lukas Rieder, Katharina Ebner, Anton Glieder and Morten Sørlie
    Citation: Biotechnology for Biofuels 2021 14:122
    Content type: Research Published on:

  14. Chlamydomonas reinhardtii is a model green alga strain for molecular studies; its fully sequenced genome has enabled omic-based analyses that have been applied to better understand its metabolic responses to stre...

    Authors: E. Hounslow, C. A. Evans, J. Pandhal, T. Sydney, N. Couto, T. K. Pham, D. James Gilmour and P. C. Wright
    Citation: Biotechnology for Biofuels 2021 14:121
    Content type: Research Published on:

  15. Lignocellulolytic enzymes are essential for agricultural waste disposal and production of renewable bioenergy. Many commercialized cellulase mixtures have been developed, mostly from saprophytic or endophytic ...

    Authors: Meng-Chun Lin, Hsion-Wen Kuo, Mu-Rong Kao, Wen-Dar Lin, Chen-Wei Li, Kuo-Sheng Hung, Sheng-Chih Yang, Su-May Yu and Tuan-Hua David Ho
    Citation: Biotechnology for Biofuels 2021 14:120
    Content type: Research Published on:

  16. The algal lipids-based biodiesel, albeit having advantages over plant oils, still remains high in the production cost. Co-production of value-added products with lipids has the potential to add benefits and is...

    Authors: Yu Zhang, Ying Ye, Fan Bai and Jin Liu
    Citation: Biotechnology for Biofuels 2021 14:119
    Content type: Review Published on:

  17. Xylan is the most abundant hemicellulose polysaccharide in nature, which can be converted into high value-added products. However, its recalcitrance to breakdown requires the synergistic action of multiple enz...

    Authors: Shu Zhang, Sha Zhao, Weihao Shang, Zijuan Yan, Xiuyun Wu, Yingjie Li, Guanjun Chen, Xinli Liu and Lushan Wang
    Citation: Biotechnology for Biofuels 2021 14:118
    Content type: Research Published on:

  18. The interest in using methanol as a substrate to cultivate acetogens increased in recent years since it can be sustainably produced from syngas and has the additional benefit of reducing greenhouse gas emissio...

    Authors: Maximilian Flaiz, Gideon Ludwig, Frank R. Bengelsdorf and Peter Dürre
    Citation: Biotechnology for Biofuels 2021 14:117
    Content type: Research Published on:

  19. Mass spectrometry-based proteomics can identify and quantify thousands of proteins from individual microbial species, but a significant percentage of these proteins are unannotated and hence classified as prot...

    Authors: Suresh Poudel, Alexander L. Cope, Kaela B. O’Dell, Adam M. Guss, Hyeongmin Seo, Cong T. Trinh and Robert L. Hettich
    Citation: Biotechnology for Biofuels 2021 14:116
    Content type: Research Published on:

  21. Plant-biomass-based nanomaterials have attracted great interest recently for their potential to replace petroleum-sourced polymeric materials for sustained economic development. However, challenges associated ...

    Authors: J. Y. Zhu, Umesh P. Agarwal, Peter N. Ciesielski, Michael E. Himmel, Runan Gao, Yulin Deng, Maria Morits and Monika Österberg
    Citation: Biotechnology for Biofuels 2021 14:114
    Content type: Review Published on:

  22. Lipids are important precursors in the biofuel and oleochemical industries. Yarrowia lipolytica is among the most extensively studied oleaginous microorganisms and has been a focus of metabolic engineering to imp...

    Authors: Annapurna Kamineni, Andrew L. Consiglio, Kyle MacEwen, Shuyan Chen, Gamuchirai Chifamba, A. Joe Shaw and Vasiliki Tsakraklides
    Citation: Biotechnology for Biofuels 2021 14:113
    Content type: Research Published on:

  23. Zymomonas mobilis is an aerotolerant α-proteobacterium, which has been genetically engineered for industrial purposes for decades. However, a comprehensive comparison of existing strains on the genomic level in c...

    Authors: Magdalena M. Felczak, Robert M. Bowers, Tanja Woyke and Michaela A. TerAvest
    Citation: Biotechnology for Biofuels 2021 14:112
    Content type: Research Published on:

  25. The biomanufacturing of d-glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for d-

    Authors: Chaofeng Li, Xiaofeng Lin, Xing Ling, Shuo Li and Hao Fang
    Citation: Biotechnology for Biofuels 2021 14:110
    Content type: Research Published on:

  26. An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equiv...

    Authors: Hengkai Meng, Wei Zhang, Huawei Zhu, Fan Yang, Yanping Zhang, Jie Zhou and Yin Li
    Citation: Biotechnology for Biofuels 2021 14:109
    Content type: Research Published on:

  27. Lignin peroxidases catalyze a variety of reactions, resulting in cleavage of both β-O-4′ ether bonds and C–C bonds in lignin, both of which are essential for depolymerizing lignin into fragments amendable to b...

    Authors: Le Thanh Mai Pham, Kai Deng, Trent R. Northen, Steven W. Singer, Paul D. Adams, Blake A. Simmons and Kenneth L. Sale
    Citation: Biotechnology for Biofuels 2021 14:108
    Content type: Research Published on:

  29. During the biomass-to-bio-oil conversion process, many studies focus on studying the association between biomass and bio-products using near-infrared spectra (NIR) and chemical analysis methods. However, the c...

    Authors: Chao Yin, Xiaohua Deng, Zhiqiang Yu, Zechun Liu, Hongxiang Zhong, Ruting Chen, Guohua Cai, Quanxing Zheng, Xiucai Liu, Jiawei Zhong, Pengfei Ma, Wei He, Kai Lin, Qiaoling Li and Anan Wu
    Citation: Biotechnology for Biofuels 2021 14:106
    Content type: Research Published on:

  30. On-site enzyme production using Trichoderma reesei can improve yields and lower the overall cost of lignocellulose saccharification by exploiting the fungal gene regulatory mechanism that enables it to continuous...

    Authors: Vera Novy, Fredrik Nielsen, Daniel Cullen, Grzegorz Sabat, Carl J. Houtman and Christopher G. Hunt
    Citation: Biotechnology for Biofuels 2021 14:105
    Content type: Research Published on:

  31. Recirculating aquaculture systems (RAS) are an essential component of sustainable inland seafood production. Still, nutrient removal from these systems can result in substantial environmental problems, or pres...

    Authors: Andreas Heyland, Jordan Roszell, Jeremy Chau, Kevin Chai, Andrew Eaton, Kathleen Nolan, Kyle Madden and Wael H. Ahmed
    Citation: Biotechnology for Biofuels 2021 14:104
    Content type: Research Published on:

  32. Understanding how the digestibility of lignocellulosic biomass is affected by its morphology is essential to design efficient processes for biomass deconstruction. In this study, we used a model based on a set...

    Authors: Jessica C. Rohrbach and Jeremy S. Luterbacher
    Citation: Biotechnology for Biofuels 2021 14:103
    Content type: Research Published on:

  33. Plant biomass is a highly abundant renewable resource that can be converted into several types of high-value-added products, including chemicals, biofuels and advanced materials. In the last few decades, an in...

    Authors: Peng Ning, Guofeng Yang, Lihong Hu, Jingxin Sun, Lina Shi, Yonghong Zhou, Zhaobao Wang and Jianming Yang
    Citation: Biotechnology for Biofuels 2021 14:102
    Content type: Review Published on:

  34. Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biof...

    Authors: James Kirby, Gina M. Geiselman, Junko Yaegashi, Joonhoon Kim, Xun Zhuang, Mary Bao Tran-Gyamfi, Jan-Philip Prahl, Eric R. Sundstrom, Yuqian Gao, Nathalie Munoz, Kristin E. Burnum-Johnson, Veronica T. Benites, Edward E. K. Baidoo, Anna Fuhrmann, Katharina Seibel, Bobbie-Jo M. Webb-Robertson…
    Citation: Biotechnology for Biofuels 2021 14:101
    Content type: Research Published on:

  35. 4-Hydroxyphenylacetic acid (4HPAA) is an important raw material for the synthesis of drugs, pesticides and biochemicals. Microbial biotechnology would be an attractive approach for 4HPAA production, and cofact...

    Authors: Yu-Ping Shen, Yu-Ling Liao, Qian Lu, Xin He, Zhi-Bo Yan and Jian-Zhong Liu
    Citation: Biotechnology for Biofuels 2021 14:100
    Content type: Research Published on:

  36. Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. The starch content can be promoted by plant growth regulators after the vegetative reproduc...

    Authors: Yerong Zhu, Xiaoxue Li, Xuan Gao, Jiqi Sun, Xiaoyuan Ji, Guodong Feng, Guangshuang Shen, Beibei Xiang and Yong Wang
    Citation: Biotechnology for Biofuels 2021 14:99
    Content type: Research Published on:

  37. Miscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity and low chemical input requirements. Within an interspecific Miscanthus cross, progeny with high biomass yield were s...

    Authors: Jose J. De Vega, Ned Peel, Sarah J. Purdy, Sarah Hawkins, Lain Donnison, Sarah Dyer and Kerrie Farrar
    Citation: Biotechnology for Biofuels 2021 14:98
    Content type: Research Published on:

  38. Xylo-oligosaccharide is the spotlight of functional sugar that improves the economic benefits of lignocellulose biorefinery. Acetic acid acidolysis technology provides a promising application for xylo-oligosac...

    Authors: Jianming Guo, Kaixuan Huang, Rou Cao, Junhua Zhang and Yong Xu
    Citation: Biotechnology for Biofuels 2021 14:97
    Content type: Research Published on:

  39. Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydra...

    Authors: Fernanda Lopes de Figueiredo, Ana Carolina Piva de Oliveira, Cesar Rafael Fanchini Terrasan, Thiago Augusto Gonçalves, Jaqueline Aline Gerhardt, Geizecler Tomazetto, Gabriela Felix Persinoti, Marcelo Ventura Rubio, Jennifer Andrea Tamayo Peña, Michelle Fernandes Araújo, Maria Augusta de Carvalho Silvello, Telma Teixeira Franco, Sarita Cândida Rabelo, Rosana Goldbeck, Fabio Marcio Squina and André Damasio
    Citation: Biotechnology for Biofuels 2021 14:96
    Content type: Research Published on:

  40. Rhodosporidium strain, a well-known oleaginous yeast, has been widely used as a platform for lipid and carotenoid production. However, the production of squalene for application in lipid-based biofuels is not rep...

    Authors: Shahryar Shakeri, Farshad Khoshbasirat and Mahmood Maleki
    Citation: Biotechnology for Biofuels 2021 14:95
    Content type: Research Published on:

  41. Seed germination is the most important stage for the formation of a new plant. This process starts when the dry seed begins to absorb water and ends when the radicle protrudes. The germination rate of seed fro...

    Authors: Bingchao Wu, Min Sun, Huan Zhang, Dan Yang, Chuang Lin, Imran Khan, Xiaoshan Wang, Xinquan Zhang, Gang Nie, Guangyan Feng, Yanhong Yan, Zhou Li, Yan Peng and Linkai Huang
    Citation: Biotechnology for Biofuels 2021 14:94
    Content type: Research Published on:

  42. Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild-typ...

    Authors: Enrique Romero-Frasca, Sharon B. Velasquez-Orta, Viviana Escobar-Sánchez, Raunel Tinoco-Valencia and María Teresa Orta Ledesma
    Citation: Biotechnology for Biofuels 2021 14:93
    Content type: Research Published on:

  43. Presence of inhibitory chemicals in lignocellulose hydrolysates is a major hurdle for production of second-generation bioethanol. Especially cheaper pre-treatment methods that ensure an economical viable produ...

    Authors: Gert Vanmarcke, Mekonnen M. Demeke, Maria R. Foulquié-Moreno and Johan M. Thevelein
    Citation: Biotechnology for Biofuels 2021 14:92
    Content type: Research Published on:

  44. Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was to investigate the growth profiles of C. kronotskyensis in the presenc...

    Authors: Thitiwut Vongkampang, Krishnan Sreenivas, Jonathan Engvall, Carl Grey and Ed W. J. van Niel
    Citation: Biotechnology for Biofuels 2021 14:91
    Content type: Research Published on:

  46. Burning fast-growing trees for energy production can be an effective alternative to coal combustion. Thus, lignocellulosic material, which can be used to obtain chemicals with a high added value, is highly abu...

    Authors: Alberto Palma, Javier Mauricio Loaiza, Manuel J. Díaz, Juan Carlos García, Inmaculada Giráldez and Francisco López
    Citation: Biotechnology for Biofuels 2021 14:89
    Content type: Research Published on:

  47. Cocksfoot grass (Dactylis glomerata L.) with high biomass yield and rich cellulose can be used to produce bioethanol as fuel additive. In view of this, ultrasonic and hydrothermal pretreatments followed by succes...

    Authors: Shao-Chao Sun, Dan Sun and Xue-Fei Cao
    Citation: Biotechnology for Biofuels 2021 14:88
    Content type: Research Published on:

  48. Global issues such as environmental problems and food security are currently of concern to all of us. Circular bioeconomy is a promising approach towards resolving these global issues. The production of bioene...

    Authors: Hui Yi Leong, Chih-Kai Chang, Kuan Shiong Khoo, Kit Wayne Chew, Shir Reen Chia, Jun Wei Lim, Jo-Shu Chang and Pau Loke Show
    Citation: Biotechnology for Biofuels 2021 14:87
    Content type: Review Published on:

  49. Bioethanol from abundant and inexpensive agricultural and industrial wastes possesses the potential to reduce greenhouse gas emissions. Bioethanol as renewable fuel addresses elevated production costs, as well...

    Authors: Anita Ejiro Nwaefuna, Karl Rumbold, Teun Boekhout and Nerve Zhou
    Citation: Biotechnology for Biofuels 2021 14:86
    Content type: Review Published on:

  50. Wheat straw, one of the most abundant agricultural residues worldwide, can be used to produce biogas, which is considered one of the most efficiently produced renewable energies. Wheat grown with the dual-purp...

    Authors: Nadia Gabbanelli, Elisa Erbetta, Maria Eugenia Sanz Smachetti, Máximo Lorenzo, Paola Mónica Talia, Ignacio Ramírez, Magdalena Vera, Ignacio Durruty, Ana Clara Pontaroli and Maria Mercedes Echarte
    Citation: Biotechnology for Biofuels 2021 14:85
    Content type: Research Published on:
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