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  1. Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Framew...

    Authors: Zi-Wen Zhou, Chun-Xian Cai, Xiu Xing, Jun Li, Zu-E. Hu, Zong-Bo Xie, Na Wang and Xiao-Qi Yu
    Citation: Biotechnology for Biofuels 2021 14:156
    Content type: Research Published on:

  2. Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals and other bioproducts, but challenged by various harsh industrial conditions, such ...

    Authors: Yanfang Liu, Yuping Lin, Yufeng Guo, Fengli Wu, Yuanyuan Zhang, Xianni Qi, Zhen Wang and Qinhong Wang
    Citation: Biotechnology for Biofuels 2021 14:155
    Content type: Research Published on:

  3. The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem fu...

    Authors: Sivasamy Sethupathy, Gabriel Murillo Morales, Yixuan Li, Yongli Wang, Jianxiong Jiang, Jianzhong Sun and Daochen Zhu
    Citation: Biotechnology for Biofuels 2021 14:154
    Content type: Review Published on:

  4. Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed...

    Authors: Leila Khaleghipour, Javier A. Linares-Pastén, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari and Eva Nordberg Karlsson
    Citation: Biotechnology for Biofuels 2021 14:153
    Content type: Research Published on:

  5. Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvested because of rapid sucrose losses and deterioration of stalks. This study was condu...

    Authors: Na Peng, Ziting Yao, Ziting Wang, Jiangfeng Huang, Muhammad Tahir Khan, Baoshan Chen and Muqing Zhang
    Citation: Biotechnology for Biofuels 2021 14:152
    Content type: Research Published on:

  6. Hydrogen peroxide–acetic acid (HPAA) is widely used in pretreatment of lignocellulose because it has a good capability in selective delignification. However, high concentration (more than 60%) of HPAA increase...

    Authors: Peiyao Wen, Ying Zhang, Junjun Zhu, Yong Xu and Junhua Zhang
    Citation: Biotechnology for Biofuels 2021 14:151
    Content type: Research Published on:

  7. Ascomycetous yeasts from the kingdom fungi inhabit every biome in nature. While filamentous fungi have been studied extensively regarding their enzymatic degradation of the complex polymers comprising lignocel...

    Authors: Jonas L. Ravn, Martin K. M. Engqvist, Johan Larsbrink and Cecilia Geijer
    Citation: Biotechnology for Biofuels 2021 14:150
    Content type: Research Published on:

  8. In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level of gene expression. Therefore, rational fine-tuning of promote...

    Authors: Yu Zhao, Shiqi Liu, Zhihui Lu, Baixiang Zhao, Shuhui Wang, Cuiying Zhang, Dongguang Xiao, Jee Loon Foo and Aiqun Yu
    Citation: Biotechnology for Biofuels 2021 14:149
    Content type: Research Published on:

  9. Lipomyces starkeyi has been widely regarded as a promising oleaginous yeast with broad industrial application prospects because of its wide substrate spectrum, good adaption to fermentation inhibitors, excellent ...

    Authors: Wei Zhou, Yanan Wang, Junlu Zhang, Man Zhao, Mou Tang, Wenting Zhou and Zhiwei Gong
    Citation: Biotechnology for Biofuels 2021 14:148
    Content type: Research Published on:

  10. Plant monoterpenoids with structural diversities have extensive applications in food, cosmetics, pharmaceuticals, and biofuels. Due to the strong dependence on the geographical locations and seasonal annual gr...

    Authors: Dengwei Lei, Zetian Qiu, Jianjun Qiao and Guang-Rong Zhao
    Citation: Biotechnology for Biofuels 2021 14:147
    Content type: Review Published on:

  11. The model ethanologenic bacterium Zymomonas mobilis has many advantages for diverse biochemical production. Although the impact of temperature especially high temperature on the growth and ethanol production of Z...

    Authors: Runxia Li, Wei Shen, Yongfu Yang, Jun Du, Mian Li and Shihui Yang
    Citation: Biotechnology for Biofuels 2021 14:146
    Content type: Research Published on:

  12. Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology indus...

    Authors: Zhennan Jiang, Zhiyong Cui, Ziwei Zhu, Yinghang Liu, Ya-jie Tang, Jin Hou and Qingsheng Qi
    Citation: Biotechnology for Biofuels 2021 14:145
    Content type: Research Published on:

  13. Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite genetic modification of major wa...

    Authors: Zhen Hu, Youmei Wang, Jingyuan Liu, Yuqi Li, Yanting Wang, Jiangfeng Huang, Yuanhang Ai, Peng Chen, Yuqing He, Muhammad Nauman Aftab, Lingqiang Wang and Liangcai Peng
    Citation: Biotechnology for Biofuels 2021 14:144
    Content type: Research Published on:

  14. During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. H...

    Authors: Wenqian Lin, Jinlai Yang, Yayue Zheng, Caoxing Huang and Qiang Yong
    Citation: Biotechnology for Biofuels 2021 14:143
    Content type: Research Published on:

  15. In plants, a large diversity of polysaccharides comprise the cell wall. Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions ...

    Authors: William J. Barnes, Sabina Koj, Ian M. Black, Stephanie A. Archer-Hartmann, Parastoo Azadi, Breeanna R. Urbanowicz, Maria J. Peña and Malcolm A. O’Neill
    Citation: Biotechnology for Biofuels 2021 14:142
    Content type: Methodology Published on:

  16. Salix (willow) species represent an important source of bioenergy and offer great potential for producing biofuels. Salix spp. like many hardwoods, produce tension wood (TW) characterized by special fibres (G-fib...

    Authors: Jie Gao, Mohamed Jebrane, Nasko Terziev and Geoffrey Daniel
    Citation: Biotechnology for Biofuels 2021 14:141
    Content type: Research Published on:

  17. The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses. In the past 5 years, exciting possibilities have emerged in the engineering ...

    Authors: Madalen Robert, Julian Waldhauer, Fabian Stritt, Bo Yang, Markus Pauly and Cătălin Voiniciuc
    Citation: Biotechnology for Biofuels 2021 14:140
    Content type: Research Published on:

  19. Biomass valorization has been suggested as a sustainable alternative to petroleum-based energy and commodities. In this context, the copper radical oxidases (CROs) from Auxiliary Activity Family 5/Subfamily 2 ...

    Authors: Maria Cleveland, Mickael Lafond, Fan Roderick Xia, Ryan Chung, Paul Mulyk, Jason E. Hein and Harry Brumer
    Citation: Biotechnology for Biofuels 2021 14:138
    Content type: Research Published on:

  20. Cellulose is not only a common component in vascular plants, but also has great economic benefits for paper, wood, and industrial products. In addition, its biosynthesis is highly regulated by carbohydrate met...

    Authors: Jing Su, Chunxia Zhang, Lingcheng Zhu, Nanxiang Yang, Jingjing Yang, Baiquan Ma, Fengwang Ma and Mingjun Li
    Citation: Biotechnology for Biofuels 2021 14:137
    Content type: Research Published on:

  21. Ethanol organosolv (EOS) pretreatment is one of the most efficient methods for boosting biomass saccharification as it can achieve an efficient fractionation of three major constituents in lignocellulose. Howe...

    Authors: Qiulu Chu, Wenyao Tong, Jianqiang Chen, Shufang Wu, Yongcan Jin, Jinguang Hu and Kai Song
    Citation: Biotechnology for Biofuels 2021 14:136
    Content type: Research Published on:

  22. Pretreatment is a critical step required for efficient conversion of woody biomass into biofuels and platform chemicals. Fungal pretreatment is regarded as one of the most promising technology for woody biomas...

    Authors: Jiangshan Ma, Huimin Yue, Hongqian Li, Jing Zhang, Yanghong Zhang, Xiaoling Wang, Si Gong and Gao-Qiang Liu
    Citation: Biotechnology for Biofuels 2021 14:135
    Content type: Research Published on:

  23. Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-d-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymeri...

    Authors: Mario Klimacek, Chao Zhong and Bernd Nidetzky
    Citation: Biotechnology for Biofuels 2021 14:134
    Content type: Research Published on:

  24. Microalgae-based high-density fuels offer an efficient and environmental pathway towards decarbonization of the transport sector and could be produced as part of a globally distributed network without competin...

    Authors: John Roles, Jennifer Yarnold, Karen Hussey and Ben Hankamer
    Citation: Biotechnology for Biofuels 2021 14:133
    Content type: Research Published on:

  25. Microalgae can contribute to more than 40% of global primary biomass production and are suitable candidates for various biotechnology applications such as food, feed products, drugs, fuels, and wastewater trea...

    Authors: Mingcan Wu, Ming Du, Guimei Wu, Feimiao Lu, Jing Li, Anping Lei, Hui Zhu, Zhangli Hu and Jiangxin Wang
    Citation: Biotechnology for Biofuels 2021 14:132
    Content type: Research Published on:

  26. Biomass-degrading enzymes with improved activity and stability can increase substrate saccharification and make biorefineries economically feasible. Filamentous fungi are a rich source of carbohydrate-active e...

    Authors: Monika Tõlgo, Silvia Hüttner, Peter Rugbjerg, Nguyen Thanh Thuy, Vu Nguyen Thanh, Johan Larsbrink and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2021 14:131
    Content type: Research Published on:

  27. Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the orga...

    Authors: Miriam Edel, Gunnar Sturm, Katrin Sturm-Richter, Michael Wagner, Julia Novion Ducassou, Yohann Couté, Harald Horn and Johannes Gescher
    Citation: Biotechnology for Biofuels 2021 14:130
    Content type: Research Published on:

  28. The overwhelming concerns due to over exploitation of fossil resources necessitate the utilization of alternative energy resources. Biodiesel has been considered as one of the most adaptable alternative to fos...

    Authors: Mustafa Kamal Pasha, Lingmei Dai, Dehua Liu, Miao Guo and Wei Du
    Citation: Biotechnology for Biofuels 2021 14:129
    Content type: Review Published on:

  29. Dye-decolorizing peroxidases (DyPs) represent a novel family of heme peroxidases that use H2O2 as the final electron acceptor to catalyze the oxidation of various organic compounds. A DyP from Irpex lacteus F17 (

    Authors: Liuqing Li, Tao Wang, Taohua Chen, Wenhan Huang, Yinliang Zhang, Rong Jia and Chao He
    Citation: Biotechnology for Biofuels 2021 14:128
    Content type: Research Published on:

  30. Nowadays there is a strong trend towards a circular economy using lignocellulosic biowaste for the production of biofuels and other bio-based products. The use of enzymes at several stages of the production pr...

    Authors: Lisanne Hameleers, Leena Penttinen, Martina Ikonen, Léa Jaillot, Régis Fauré, Nicolas Terrapon, Peter J. Deuss, Nina Hakulinen, Emma R. Master and Edita Jurak
    Citation: Biotechnology for Biofuels 2021 14:127
    Content type: Research Published on:

  31. β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal glycoside hydrolase family 3 (GH3) β-gluc...

    Authors: Mu-Rong Kao, Su-May Yu and Tuan-H ua David Ho
    Citation: Biotechnology for Biofuels 2021 14:126
    Content type: Research Published on:

  32. During the acetogenic step of anaerobic digestion, the products of acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis and methanogenesis are highly i...

    Authors: Anna Detman, Michał Bucha, Laura Treu, Aleksandra Chojnacka, Łukasz Pleśniak, Agnieszka Salamon, Ewa Łupikasza, Robert Gromadka, Jan Gawor, Agnieszka Gromadka, Wojciech Drzewicki, Marta Jakubiak, Marek Janiga, Irena Matyasik, Mieczysław K. Błaszczyk, Mariusz Orion Jędrysek…
    Citation: Biotechnology for Biofuels 2021 14:125
    Content type: Research Published on:

  33. Microbial oils, generated from lignocellulosic material, have great potential as renewable and sustainable alternatives to fossil-based fuels and chemicals. By unravelling the diversity of lipid accumulation p...

    Authors: Jule Brandenburg, Johanna Blomqvist, Volha Shapaval, Achim Kohler, Sabine Sampels, Mats Sandgren and Volkmar Passoth
    Citation: Biotechnology for Biofuels 2021 14:124
    Content type: Research Published on:

  34. Sugarcane is one of the most crucial energy crops that produces high yields of sugar and lignocellulose. The cellulose crystallinity index (CrI) and lignin are the two kinds of key cell wall features that acco...

    Authors: Xinru Li, Fumin Ma, Chengping Liang, Maoyao Wang, Yan Zhang, Yufei Shen, Muhammad Adnan, Pan Lu, Muhammad Tahir Khan, Jiangfeng Huang and Muqing Zhang
    Citation: Biotechnology for Biofuels 2021 14:123
    Content type: Research Published on:

  35. Lytic polysaccharide monooxygenases (LPMOs) are attracting large attention due their ability to degrade recalcitrant polysaccharides in biomass conversion and to perform powerful redox chemistry.

    Authors: Lukas Rieder, Katharina Ebner, Anton Glieder and Morten Sørlie
    Citation: Biotechnology for Biofuels 2021 14:122
    Content type: Research Published on:

  36. Chlamydomonas reinhardtii is a model green alga strain for molecular studies; its fully sequenced genome has enabled omic-based analyses that have been applied to better understand its metabolic responses to stre...

    Authors: E. Hounslow, C. A. Evans, J. Pandhal, T. Sydney, N. Couto, T. K. Pham, D. James Gilmour and P. C. Wright
    Citation: Biotechnology for Biofuels 2021 14:121
    Content type: Research Published on:

  37. Lignocellulolytic enzymes are essential for agricultural waste disposal and production of renewable bioenergy. Many commercialized cellulase mixtures have been developed, mostly from saprophytic or endophytic ...

    Authors: Meng-Chun Lin, Hsion-Wen Kuo, Mu-Rong Kao, Wen-Dar Lin, Chen-Wei Li, Kuo-Sheng Hung, Sheng-Chih Yang, Su-May Yu and Tuan-Hua David Ho
    Citation: Biotechnology for Biofuels 2021 14:120
    Content type: Research Published on:

  38. The algal lipids-based biodiesel, albeit having advantages over plant oils, still remains high in the production cost. Co-production of value-added products with lipids has the potential to add benefits and is...

    Authors: Yu Zhang, Ying Ye, Fan Bai and Jin Liu
    Citation: Biotechnology for Biofuels 2021 14:119
    Content type: Review Published on:

  39. Xylan is the most abundant hemicellulose polysaccharide in nature, which can be converted into high value-added products. However, its recalcitrance to breakdown requires the synergistic action of multiple enz...

    Authors: Shu Zhang, Sha Zhao, Weihao Shang, Zijuan Yan, Xiuyun Wu, Yingjie Li, Guanjun Chen, Xinli Liu and Lushan Wang
    Citation: Biotechnology for Biofuels 2021 14:118
    Content type: Research Published on:

  40. The interest in using methanol as a substrate to cultivate acetogens increased in recent years since it can be sustainably produced from syngas and has the additional benefit of reducing greenhouse gas emissio...

    Authors: Maximilian Flaiz, Gideon Ludwig, Frank R. Bengelsdorf and Peter Dürre
    Citation: Biotechnology for Biofuels 2021 14:117
    Content type: Research Published on:

  41. Mass spectrometry-based proteomics can identify and quantify thousands of proteins from individual microbial species, but a significant percentage of these proteins are unannotated and hence classified as prot...

    Authors: Suresh Poudel, Alexander L. Cope, Kaela B. O’Dell, Adam M. Guss, Hyeongmin Seo, Cong T. Trinh and Robert L. Hettich
    Citation: Biotechnology for Biofuels 2021 14:116
    Content type: Research Published on:

  43. Plant-biomass-based nanomaterials have attracted great interest recently for their potential to replace petroleum-sourced polymeric materials for sustained economic development. However, challenges associated ...

    Authors: J. Y. Zhu, Umesh P. Agarwal, Peter N. Ciesielski, Michael E. Himmel, Runan Gao, Yulin Deng, Maria Morits and Monika Österberg
    Citation: Biotechnology for Biofuels 2021 14:114
    Content type: Review Published on:

  44. Lipids are important precursors in the biofuel and oleochemical industries. Yarrowia lipolytica is among the most extensively studied oleaginous microorganisms and has been a focus of metabolic engineering to imp...

    Authors: Annapurna Kamineni, Andrew L. Consiglio, Kyle MacEwen, Shuyan Chen, Gamuchirai Chifamba, A. Joe Shaw and Vasiliki Tsakraklides
    Citation: Biotechnology for Biofuels 2021 14:113
    Content type: Research Published on:

  45. Zymomonas mobilis is an aerotolerant α-proteobacterium, which has been genetically engineered for industrial purposes for decades. However, a comprehensive comparison of existing strains on the genomic level in c...

    Authors: Magdalena M. Felczak, Robert M. Bowers, Tanja Woyke and Michaela A. TerAvest
    Citation: Biotechnology for Biofuels 2021 14:112
    Content type: Research Published on:

  47. The biomanufacturing of d-glucaric acid has attracted increasing interest because it is one of the top value-added chemicals produced from biomass. Saccharomyces cerevisiae is regarded as an excellent host for d-

    Authors: Chaofeng Li, Xiaofeng Lin, Xing Ling, Shuo Li and Hao Fang
    Citation: Biotechnology for Biofuels 2021 14:110
    Content type: Research Published on:

  48. An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equiv...

    Authors: Hengkai Meng, Wei Zhang, Huawei Zhu, Fan Yang, Yanping Zhang, Jie Zhou and Yin Li
    Citation: Biotechnology for Biofuels 2021 14:109
    Content type: Research Published on:

  49. Lignin peroxidases catalyze a variety of reactions, resulting in cleavage of both β-O-4′ ether bonds and C–C bonds in lignin, both of which are essential for depolymerizing lignin into fragments amendable to b...

    Authors: Le Thanh Mai Pham, Kai Deng, Trent R. Northen, Steven W. Singer, Paul D. Adams, Blake A. Simmons and Kenneth L. Sale
    Citation: Biotechnology for Biofuels 2021 14:108
    Content type: Research Published on:
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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