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  1. The efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysa...

    Authors: Lei Ji, Jinshui Yang, Hua Fan, Yi Yang, Baozhen Li, Xuejian Yu, Ning Zhu and Hongli Yuan
    Citation: Biotechnology for Biofuels 2014 7:130
  2. Enzymatic hydrolysis is a crucial step of biomass conversion into biofuels and different pretreatments have been proposed to improve the process efficiency. Amongst the various factors affecting hydrolysis yie...

    Authors: Jefferson Esquina Tsuchida, Camila Alves Rezende, Rodrigo de Oliveira-Silva, Marisa Aparecida Lima, Marcel Nogueira d’Eurydice, Igor Polikarpov and Tito José Bonagamba
    Citation: Biotechnology for Biofuels 2014 7:127
  3. The expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement co...

    Authors: Mark D Harrison, Zhanying Zhang, Kylie Shand, Barrie Fong Chong, Jason Nichols, Paul Oeller, Ian M O'Hara, William OS Doherty and James L Dale
    Citation: Biotechnology for Biofuels 2014 7:131
  4. Efficient industrial processes for converting plant lignocellulosic materials into biofuels are a key to global efforts to come up with alternative energy sources to fossil fuels. Novel cellulolytic enzymes ha...

    Authors: Sebastian GA Konietzny, Phillip B Pope, Aaron Weimann and Alice C McHardy
    Citation: Biotechnology for Biofuels 2014 7:124
  5. The concept of adaptive evolution implies underlying genetic mutations conferring a selective advantage to an organism under particular environmental conditions. Thus, a flow cytometry-based strategy was used ...

    Authors: Natarajan Velmurugan, Minji Sung, Sung Sun Yim, Min S Park, Ji Won Yang and Ki Jun Jeong
    Citation: Biotechnology for Biofuels 2014 7:117
  6. The lipid content of microalgae is regarded as an important indicator for biodiesel. Many attempts have been made to increase the lipid content of microalgae through biochemical and genetic engineering. Signif...

    Authors: Jianhui Zhang, Qiang Hao, Lili Bai, Jin Xu, Weibo Yin, Liying Song, Ling Xu, Xuejie Guo, Chengming Fan, Yuhong Chen, Jue Ruan, Shanting Hao, Yuanguang Li, Richard R-C Wang and Zanmin Hu
    Citation: Biotechnology for Biofuels 2014 7:128
  7. Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. H...

    Authors: Yuping Lin, Kulika Chomvong, Ligia Acosta-Sampson, Raíssa Estrela, Jonathan M Galazka, Soo Rin Kim, Yong-Su Jin and Jamie HD Cate
    Citation: Biotechnology for Biofuels 2014 7:126
  8. Shewanella oneidensis MR-1 is one of the model microorganisms used for extracellular electron transfer. In this study, to elucidate the capability and the relevant metabolic processes of S. oneidensis MR-1 involv...

    Authors: Longfei Mao and Wynand S Verwoerd
    Citation: Biotechnology for Biofuels 2014 7:118
  9. The emergence of biofuels produced through yeast fermentation represents an important avenue for sustainable energy production. Despite all its advantages, this process is vulnerable to contamination by other ...

    Authors: Juliano Bertozzi Silva and Dominic Sauvageau
    Citation: Biotechnology for Biofuels 2014 7:123
  10. Efficient xylose fermentation by yeast would improve the economical and sustainable nature of biofuels production from lignocellulosic biomass. However, the efficiency of xylose fermentation by the yeast Saccharo...

    Authors: Sun-Mi Lee, Taylor Jellison and Hal S Alper
    Citation: Biotechnology for Biofuels 2014 7:122
  11. Wheat straw is an attractive substrate for second generation ethanol production because it will complement and augment wheat production rather than competing with food production. However, like other sources o...

    Authors: Samuel RA Collins, Nikolaus Wellner, Isabel Martinez Bordonado, Andrea L Harper, Charlotte N Miller, Ian Bancroft and Keith W Waldron
    Citation: Biotechnology for Biofuels 2014 7:121
  12. Lignocellulosic biomass from plant biomass, especially softwoods, are well-known to present difficulties during attempts at hydrolysis due to their rigid structure. Pretreatment of lignocellulosic biomass with...

    Authors: Kazuma Ogura, Kazuaki Ninomiya, Kenji Takahashi, Chiaki Ogino and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2014 7:120
  13. Hydrogen is regarded as an attractive future energy carrier for its high energy content and zero CO2 emission. Currently, the majority of hydrogen is generated from fossil fuels. However, from an environmental pe...

    Authors: Zhicheng Lai, Muzi Zhu, Xiaofeng Yang, Jufang Wang and Shuang Li
    Citation: Biotechnology for Biofuels 2014 7:119
  14. The recalcitrance of lignocellulosic biomass is a major limitation for its conversion into biofuels by enzymatic hydrolysis. The use of a pretreatment technology is an essential step to diminish biomass recalc...

    Authors: Shaoni Sun, Xuefei Cao, Shaolong Sun, Feng Xu, Xianliang Song, Run-Cang Sun and Gwynn Lloyd Jones
    Citation: Biotechnology for Biofuels 2014 7:116
  15. Understanding the diversity of lignocellulose-degrading enzymes in nature will provide insights for the improvement of cellulolytic enzyme cocktails used in the biofuels industry. Two families of enzymes, fung...

    Authors: Adam J Book, Ragothaman M Yennamalli, Taichi E Takasuka, Cameron R Currie, George N Phillips Jr and Brian G Fox
    Citation: Biotechnology for Biofuels 2014 7:109
  16. We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze metha...

    Authors: Jinjin Huang, Ji Xia, Zhen Yang, Feifei Guan, Di Cui, Guohua Guan, Wei Jiang and Ying Li
    Citation: Biotechnology for Biofuels 2014 7:111
  17. The search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. The present work reports the heterologous expres...

    Authors: Liliane FC Ribeiro, Rosymar C De Lucas, Gabriela L Vitcosque, Lucas F Ribeiro, Richard J Ward, Marcelo V Rubio, Andre RL Damásio, Fabio M Squina, Rebecca C Gregory, Paul H Walton, João A Jorge, Rolf A Prade, Marcos S Buckeridge and Maria de Lourdes TM Polizeli
    Citation: Biotechnology for Biofuels 2014 7:115
  18. The European Union has made it a strategic objective to develop its biofuels market in order to minimize greenhouse gas (GHG) emissions, to help mitigate climate change and to address energy insecurity within ...

    Authors: Jade Littlewood, Miao Guo, Wout Boerjan and Richard J Murphy
    Citation: Biotechnology for Biofuels 2014 7:113
  19. Ligninolytic peroxidases are divided into three families: manganese peroxidases (MnPs), lignin peroxidases (LiPs), and versatile peroxidases (VPs). The latter two are able to degrade intact lignins, as shown u...

    Authors: Elena Fernández-Fueyo, Francisco J Ruiz-Dueñas and Angel T Martínez
    Citation: Biotechnology for Biofuels 2014 7:114
  20. Lactobacillus plantarum is an attractive candidate for metabolic engineering towards bioprocessing of lignocellulosic biomass to ethanol or polylactic acid, as its natural characteristics include high ethanol and...

    Authors: Sarah Moraïs, Naama Shterzer, Raphael Lamed, Edward A Bayer and Itzhak Mizrahi
    Citation: Biotechnology for Biofuels 2014 7:112
  21. High-cost production of bioplastics polyhydroxyalkanoates (PHA) is a major concern for their large scale application. In order to produce PHA economically, new technology must be developed to reduce costs on e...

    Authors: Haitao Yue, Chen Ling, Tao Yang, Xiangbin Chen, Yuling Chen, Haiteng Deng, Qiong Wu, Jinchun Chen and Guo-Qiang Chen
    Citation: Biotechnology for Biofuels 2014 7:108
  22. Microalgae are ideal raw materials for biodiesel and bioactive compounds. Glycerol-3-phosphate is formed from dihydroxyacetone phosphate (DHAP) through the glycolytic pathway catalyzed by glycerol-3-phosphate ...

    Authors: Yao Yao, Yang Lu, Kun-Tao Peng, Tan Huang, Ying-Fang Niu, Wei-Hong Xie, Wei-Dong Yang, Jie-Sheng Liu and Hong-Ye Li
    Citation: Biotechnology for Biofuels 2014 7:110
  23. Cellulose is the most abundant biomass on earth. The major players in cellulose degradation in nature are cellulases produced by microorganisms. Aerobic filamentous fungi are the main sources of commercial cel...

    Authors: Zheng Zhang, Jun-Liang Liu, Jian-Yi Lan, Cheng-Jie Duan, Qing-Sheng Ma and Jia-Xun Feng
    Citation: Biotechnology for Biofuels 2014 7:107
  24. 2,3,5,6-Tetramethylpyrazine (TMP) is a popular food flavour additive. This biologically active ingredient has additional potential dietotherapy functions, such as in cardiovascular and cerebrovascular health. ...

    Authors: Zijun Xiao, Xiaoyuan Hou, Xin Lyu, Lijun Xi and Jing-yi Zhao
    Citation: Biotechnology for Biofuels 2014 7:106
  25. The economics of producing only electricity from residues, which comprise of surplus bagasse and 50% post-harvest residues, at an existing sugar mill in South Africa was compared to the coproduction of ethanol...

    Authors: Abdul M Petersen, Mathew C Aneke and Johann F Görgens
    Citation: Biotechnology for Biofuels 2014 7:105
  26. One of the challenges facing the fuel ethanol industry is the management of bacterial contamination during fermentation. Lactobacillus species are the predominant contaminants that decrease the profitability of b...

    Authors: Piyum A Khatibi, Dwayne R Roach, David M Donovan, Stephen R Hughes and Kenneth M Bischoff
    Citation: Biotechnology for Biofuels 2014 7:104
  27. Engineered fungi are attractive platforms for the production of plant cell wall hydrolytic enzymes which, among other biotechnological applications, are required for the efficient conversion of biomass to gluc...

    Authors: Juan Antonio Tamayo-Ramos and Margarita Orejas
    Citation: Biotechnology for Biofuels 2014 7:103
  28. Two major hurdles for successful production of second-generation bioethanol are the presence of inhibitory compounds in lignocellulosic media, and the fact that Saccharomyces cerevisiae cannot naturally utilise p...

    Authors: Johan O Westman, Nicklas Bonander, Mohammad J Taherzadeh and Carl Johan Franzén
    Citation: Biotechnology for Biofuels 2014 7:102
  29. Biosynthesis of liquid fuels and biomass-based building block chemicals from microorganisms have been regarded as a competitive alternative route to traditional. Zymomonas mobilis possesses a number of desirable ...

    Authors: Ming Xiong He, Bo Wu, Han Qin, Zhi Yong Ruan, Fu Rong Tan, Jing Li Wang, Zong Xia Shui, Li Chun Dai, Qi Li Zhu, Ke Pan, Xiao Yu Tang, Wen Guo Wang and Qi Chun Hu
    Citation: Biotechnology for Biofuels 2014 7:101
  30. Clostridium clariflavum is an anaerobic, thermophilic, Gram-positive bacterium, capable of growth on crystalline cellulose as a single carbon source. The genome of C. clariflavum has been sequenced to completion,...

    Authors: Lior Artzi, Bareket Dassa, Ilya Borovok, Melina Shamshoum, Raphael Lamed and Edward A Bayer
    Citation: Biotechnology for Biofuels 2014 7:100
  31. Molecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chem...

    Authors: S Andreas Angermayr, Aniek D van der Woude, Danilo Correddu, Angie Vreugdenhil, Valeria Verrone and Klaas J Hellingwerf
    Citation: Biotechnology for Biofuels 2014 7:99
  32. The deconstruction of renewable biomass feedstocks into soluble sugars at low cost is a critical component of the biochemical conversion of biomass to fuels and chemicals. Providing low cost high concentration...

    Authors: Xiaowen Chen, Joseph Shekiro, Thomas Pschorn, Marc Sabourin, Ling Tao, Rick Elander, Sunkyu Park, Ed Jennings, Robert Nelson, Olev Trass, Keith Flanegan, Wei Wang, Michael E Himmel, David Johnson and Melvin P Tucker
    Citation: Biotechnology for Biofuels 2014 7:98
  33. Biodiesel production from marine microalgae has received much attention as microalgae can be cultivated on non-arable land without the use of potable water, and with the additional benefits of mitigating CO2 emis...

    Authors: Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2014 7:97
  34. Camelina sativa (L.) Crantz, known by such popular names as “gold-of-pleasure” and “false flax,” is an alternative oilseed crop for biofuel production and can be grown in harsh environments. Considerable interest...

    Authors: Won Park, Yufeng Feng and Sung-Ju Ahn
    Citation: Biotechnology for Biofuels 2014 7:96
  35. Ensiling may act as a pretreatment of fresh grass biomass and increase the enzymatic conversion of structural carbohydrates to fermentable sugars. However, ensiling does not provide sufficient severity to be a...

    Authors: Morten Ambye-Jensen, Katja S Johansen, Thomas Didion, Zsófia Kádár and Anne S Meyer
    Citation: Biotechnology for Biofuels 2014 7:95
  36. In order to rapidly and efficiently screen potential biofuel feedstock candidates for quintessential traits, robust high-throughput analytical techniques must be developed and honed. The traditional methods of...

    Authors: Jason S Lupoi, Seema Singh, Mark Davis, David J Lee, Merv Shepherd, Blake A Simmons and Robert J Henry
    Citation: Biotechnology for Biofuels 2014 7:93
  37. Mixed microbial cultures, in which bacteria and fungi interact, have been proposed as an efficient way to deconstruct plant waste. The characterization of specific microbial consortia could be the starting poi...

    Authors: Diego Javier Jiménez, Francisco Dini-Andreote and Jan Dirk van Elsas
    Citation: Biotechnology for Biofuels 2014 7:92
  38. Jatropha curcas is being promoted as a new bioenergy crop in tropical and subtropical regions due to its high amount of seed oil and its potential capacity to grow on marginal land for biofuel production. However...

    Authors: Jian Ye, Yunfeng Geng, Bipei Zhang, Huizhu Mao, Jing Qu and Nam-Hai Chua
    Citation: Biotechnology for Biofuels 2014 7:91
  39. Previous research focused on pretreatment of biomass, production of fermentable sugars and their consumption to produce ethanol. The main goal of the work was to economise the production process cost of fermen...

    Authors: Reinu E Abraham, Madan L Verma, Colin J Barrow and Munish Puri
    Citation: Biotechnology for Biofuels 2014 7:90
  40. Butanol production directly from CO2 in photosynthetic cyanobacteria is restricted by the high toxicity of butanol to the hosts. In previous studies, we have found that a few two-component signal transduction sys...

    Authors: Lei Chen, Lina Wu, Jiangxin Wang and Weiwen Zhang
    Citation: Biotechnology for Biofuels 2014 7:89
  41. Oxygenic photosynthetic microorganisms such as cyanobacteria and microalgae have attracted attention as an alternative carbon source for the next generation of biofuels. Glycogen abundantly accumulated in cyan...

    Authors: Shimpei Aikawa, Atsumi Nishida, Shih-Hsin Ho, Jo-Shu Chang, Tomohisa Hasunuma and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2014 7:88
  42. There is still considerable debate regarding the actual mechanism by which a “cellulase mixture” deconstructs cellulosic materials, with accessibility to the substrate at the microscopic level being one of the...

    Authors: Valdeir Arantes, Keith Gourlay and Jack N Saddler
    Citation: Biotechnology for Biofuels 2014 7:87
  43. Ionic liquid (IL) pretreatment could enable an economically viable route to produce biofuels by providing efficient means to extract sugars and lignin from lignocellulosic biomass. However, to realize this, no...

    Authors: NVSN Murthy Konda, Jian Shi, Seema Singh, Harvey W Blanch, Blake A Simmons and Daniel Klein-Marcuschamer
    Citation: Biotechnology for Biofuels 2014 7:86
  44. Cellobiose and xylose co-fermentation holds promise for efficiently producing biofuels from plant biomass. Cellobiose phosphorylase (CBP), an intracellular enzyme generally found in anaerobic bacteria, cleaves...

    Authors: Kulika Chomvong, Vesna Kordić, Xin Li, Stefan Bauer, Abigail E Gillespie, Suk-Jin Ha, Eun Joong Oh, Jonathan M Galazka, Yong-Su Jin and Jamie H D Cate
    Citation: Biotechnology for Biofuels 2014 7:85
  45. Chlorella is one of the few microalgae employed for human consumption. It typically has a high protein content, but it can also accumulate high amounts of lipids or carbohydrates under stress conditions and, for ...

    Authors: Alessia Guccione, Natascia Biondi, Giacomo Sampietro, Liliana Rodolfi, Niccolò Bassi and Mario R Tredici
    Citation: Biotechnology for Biofuels 2014 7:84
  46. Biofuels are a well-known alternative to the largely used fossil-derived fuels, however the competition with food production is an ethical dilemma. Fortunately a solution is offered by second-generation biofue...

    Authors: Rafael C Bernardi, Isaac Cann and Klaus Schulten
    Citation: Biotechnology for Biofuels 2014 7:83
  47. Consolidated bioprocessing (CBP) of lignocellulosic biomass to hydrogen offers great potential for lower cost and higher efficiency compared to processes featuring dedicated cellulase production. Current studi...

    Authors: Guang-Li Cao, Lei Zhao, Ai-Jie Wang, Zhen-Yu Wang and Nan-Qi Ren
    Citation: Biotechnology for Biofuels 2014 7:82
  48. Sterols are vital structural and regulatory components in eukaryotic cells; however, their biosynthetic pathways and functional roles in microalgae remain poorly understood.

    Authors: Yandu Lu, Wenxu Zhou, Li Wei, Jing Li, Jing Jia, Fei Li, Steven M Smith and Jian Xu
    Citation: Biotechnology for Biofuels 2014 7:81
  49. Clostridium thermocellum is a thermophilic anaerobic bacterium that degrades cellulose by using a highly effective cellulosome, a macromolecular complex consisting of multiple cellulose degrading enzymes organize...

    Authors: Wei Hong, Jie Zhang, Yingang Feng, Georg Mohr, Alan M Lambowitz, Gu-Zhen Cui, Ya-Jun Liu and Qiu Cui
    Citation: Biotechnology for Biofuels 2014 7:80