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  1. Biological hydrogen production from lignocellulosic biomass shows great potential as a promising alternative to conventional hydrogen production methods, such as electrolysis of water and coal gasification. Cu...

    Authors: Lei Zhao, Guang-Li Cao, Ai-Jie Wang, Hong-Yu Ren, Kun Zhang and Nan-Qi Ren
    Citation: Biotechnology for Biofuels 2014 7:178
  2. The recalcitrance of softwood to enzymatic hydrolysis is one of the major bottlenecks hindering its profitable use as a raw material for platform sugars. In softwood, the guaiacyl-type lignin is especially pro...

    Authors: Ulla Moilanen, Miriam Kellock, Anikó Várnai, Martina Andberg and Liisa Viikari
    Citation: Biotechnology for Biofuels 2014 7:177
  3. Nitrogen starvation is known to cause drastic alterations in physiology and metabolism leading to the accumulation of lipid bodies in many microalgae, and it thus presents an important alternative for biofuel ...

    Authors: Luis Valledor, Takeshi Furuhashi, Luis Recuenco-Muñoz, Stefanie Wienkoop and Wolfram Weckwerth
    Citation: Biotechnology for Biofuels 2014 7:171
  4. Non-productive binding of enzymes to lignin is thought to impede the saccharification efficiency of pretreated lignocellulosic biomass to fermentable sugars. Due to a lack of suitable analytical techniques tha...

    Authors: Dahai Gao, Carolyn Haarmeyer, Venkatesh Balan, Timothy A Whitehead, Bruce E Dale and Shishir PS Chundawat
    Citation: Biotechnology for Biofuels 2014 7:175
  5. The filamentous fungus Trichoderma reesei is the main industrial cellulolytic enzyme producer. Several strains have been developed in the past using random mutagenesis, and despite impressive performance enhancem...

    Authors: Dante Poggi-Parodi, Frédérique Bidard, Aurélie Pirayre, Thomas Portnoy, Corinne Blugeon, Bernhard Seiboth, Christian P Kubicek, Stéphane Le Crom and Antoine Margeot
    Citation: Biotechnology for Biofuels 2014 7:173
  6. N-acetyl-β-D-glucosamine (GlcNAc) is widely used as a valuable pharmacological agent and a functional food additive. The traditional chemical process for GlcNAc production has some problems such as high product.....

    Authors: Xing Fu, Qiaojuan Yan, Shaoqing Yang, Xinbin Yang, Yu Guo and Zhengqiang Jiang
    Citation: Biotechnology for Biofuels 2014 7:174
  7. Oleaginous microorganisms, such as bacterium, yeast and algal species, can represent an alternative oil source for biodiesel production. The composition of their accumulated lipid is similar to the lipid of an...

    Authors: Julien Cescut, Luc Fillaudeau, Carole Molina-Jouve and Jean-Louis Uribelarrea
    Citation: Biotechnology for Biofuels 2014 7:164
  8. Flow sheet options for integrating ethanol production from spent sulfite liquor (SSL) into the acid-based sulfite pulping process at the Sappi Saiccor mill (Umkomaas, South Africa) were investigated, including...

    Authors: Abdul M Petersen, Kate Haigh and Johann F Görgens
    Citation: Biotechnology for Biofuels 2014 7:169
  9. Simultaneous saccharification and fermentation (SSF) is a promising process for bioconversion of lignocellulosic biomass. High glucan loading for hydrolysis and fermentation is an efficient approach to reduce ...

    Authors: Zhi-Hua Liu, Lei Qin, Jia-Qing Zhu, Bing-Zhi Li and Ying-Jin Yuan
    Citation: Biotechnology for Biofuels 2014 7:167
  10. The efficient conversion of lignocellulosic feedstocks remains a key step in the commercialization of biofuels. One of the barriers to cost-effective conversion of lignocellulosic biomass to sugars remains the...

    Authors: Roman Brunecky, Sarah E Hobdey, Larry E Taylor, Ling Tao, Melvin P Tucker, Michael E Himmel and Stephen R Decker
    Citation: Biotechnology for Biofuels 2014 7:170
  11. Chemical and physical pretreatment of lignocellulosic biomass improves substrate reactivity for increased microbial biofuel production, but also restricts growth via the release of furan aldehydes, such as fur...

    Authors: Sonya M Clarkson, Scott D Hamilton-Brehm, Richard J Giannone, Nancy L Engle, Timothy J Tschaplinski, Robert L Hettich and James G Elkins
    Citation: Biotechnology for Biofuels 2014 7:165
  12. Engineering of Saccharomyces cerevisiae for the simultaneous utilization of hexose and pentose sugars is vital for cost-efficient cellulosic bioethanol production. This yeast lacks specific pentose transporters a...

    Authors: Jeroen G Nijland, Hyun Yong Shin, René M de Jong, Paul P de Waal, Paul Klaassen and Arnold JM Driessen
    Citation: Biotechnology for Biofuels 2014 7:168
  13. A key focus in sustainable biofuel research is to develop cost-effective and energy-saving approaches to increase saccharification of lignocellulosic biomass. Numerous efforts have been made to identify critic...

    Authors: Jijiao Zeng, Deepak Singh, Difeng Gao and Shulin Chen
    Citation: Biotechnology for Biofuels 2014 7:161
  14. For economical bioethanol production from lignocellulosic materials, the major technical challenges to lower the production cost are as follows: (1) The microorganism should use efficiently all glucose and xyl...

    Authors: Ke-Ke Cheng, Jing Wu, Zhang-Nan Lin and Jian-An Zhang
    Citation: Biotechnology for Biofuels 2014 7:166
  15. Clostridium pasteurianum is one of the most promising biofuel producers within the genus Clostridium owing to its unique metabolic ability to ferment glycerol into butanol. Although an efficient means is availabl...

    Authors: Michael E Pyne, Murray Moo-Young, Duane A Chung and C Perry Chou
    Citation: Biotechnology for Biofuels 2014 7:163
  16. Studies in bioconversions have continuously sought the development of processing strategies to overcome the “close physical association” between plant cell wall polymers thought to significantly contribute to ...

    Authors: Michael J Selig, Lisbeth G Thygesen and Claus Felby
    Citation: Biotechnology for Biofuels 2014 7:159
  17. Agricultural residue is more efficient than purified cellulose at inducing lignocellulolytic enzyme production in Penicillium oxalicum GZ-2, but in Trichoderma reesei RUT-C30, cellulose induces a more efficient r...

    Authors: Hanpeng Liao, Shuixian Li, Zhong Wei, Qirong Shen and Yangchun Xu
    Citation: Biotechnology for Biofuels 2014 7:162
  18. Among themophilic consolidated bioprocessing (CBP) candidate organisms, environmental isolates of Clostridium clariflavum have demonstrated the ability to grow on xylan, and the genome of C. clariflavum DSM 19732...

    Authors: Javier A Izquierdo, Sivakumar Pattathil, Anna Guseva, Michael G Hahn and Lee R Lynd
    Citation: Biotechnology for Biofuels 2014 7:136
  19. Microbial lipids produced from lignocellulosic biomass hold great promise for the biodiesel industry. These lipids usually consist of three major processes: pretreatment, enzymatic hydrolysis and lipid product...

    Authors: Zhiwei Gong, Hongwei Shen, Xiaobing Yang, Qian Wang, Haibo Xie and Zongbao K Zhao
    Citation: Biotechnology for Biofuels 2014 7:158
  20. The utilization of biomass from microalgae for biofuel production is one of the key elements for the development of a sustainable and secure energy supply. Among the different microalgae, Chlorella species are of...

    Authors: Stefano Cazzaniga, Luca Dall'Osto, Joanna Szaub, Luca Scibilia, Matteo Ballottari, Saul Purton and Roberto Bassi
    Citation: Biotechnology for Biofuels 2014 7:157
  21. Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concen...

    Authors: Evert K Holwerda, Philip G Thorne, Daniel G Olson, Daniel Amador-Noguez, Nancy L Engle, Timothy J Tschaplinski, Johannes P van Dijken and Lee R Lynd
    Citation: Biotechnology for Biofuels 2014 7:155
  22. Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral ...

    Authors: Jian Ye, Jing Qu, Hui-Zhu Mao, Zhi-Gang Ma, Nur E Rahman, Chao Bai, Wen Chen, Shu-Ye Jiang, Srinivasan Ramachandran and Nam-Hai Chua
    Citation: Biotechnology for Biofuels 2014 7:149
  23. The model alga Chlamydomonas reinhardtii requires acetate as a co-substrate for optimal production of lipids, and the addition of acetate to culture media has practical and economic implications for algal biofuel...

    Authors: Jesse B Therien, Oleg A Zadvornyy, Matthew C Posewitz, Donald A Bryant and John W Peters
    Citation: Biotechnology for Biofuels 2014 7:154
  24. Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial ...

    Authors: Camilla Lambertz, Megan Garvey, Johannes Klinger, Dirk Heesel, Holger Klose, Rainer Fischer and Ulrich Commandeur
    Citation: Biotechnology for Biofuels 2014 7:135
  25. Yarrowia lipolytica is an oleaginous yeast capable of metabolizing glucose to lipids, which then accumulate intracellularly. However, it lacks the suite of cellulolytic enzymes required to break down biomass cell...

    Authors: Hui Wei, Wei Wang, Markus Alahuhta, Todd Vander Wall, John O Baker, Larry E Taylor, Stephen R Decker, Michael E Himmel and Min Zhang
    Citation: Biotechnology for Biofuels 2014 7:148
  26. Sugar cane internodes can be divided diagonally into four fractions, of which the two innermost ones are the least recalcitrant pith and the moderately accessible pith-rind interface. These fractions differ in...

    Authors: Anikó Várnai, Thales HF Costa, Craig B Faulds, Adriane MF Milagres, Matti Siika-aho and André Ferraz
    Citation: Biotechnology for Biofuels 2014 7:153
  27. Third generation biodiesel processing from microbial lipids using low-cost lignocellulosic feedstocks has attracted much attention. Endophytes isolated from oleaginous plants possibly have the capacity to accu...

    Authors: Qin Zhang, Yanbin Li and Liming Xia
    Citation: Biotechnology for Biofuels 2014 7:152
  28. Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol producti...

    Authors: Qining Sun, Marcus Foston, Xianzhi Meng, Daisuke Sawada, Sai Venkatesh Pingali, Hugh M O’Neill, Hongjia Li, Charles E Wyman, Paul Langan, Art J Ragauskas and Rajeev Kumar
    Citation: Biotechnology for Biofuels 2014 7:150
  29. Biogas is a renewable energy carrier which is used for heat and power production or, in the form of purified methane, as a vehicle fuel. The formation of methane from organic materials is carried out by a mixe...

    Authors: Linn Solli, Othilde Elise HĂĄvelsrud, Svein Jarle Horn and Anne Gunn Rike
    Citation: Biotechnology for Biofuels 2014 7:146
  30. Clostridia are anaerobic Gram-positive Firmicutes containing broad and flexible systems for substrate utilization, which have been used successfully to produce a range of industrial compounds. In particular, Clos...

    Authors: Satyakam Dash, Thomas J Mueller, Keerthi P Venkataramanan, Eleftherios T Papoutsakis and Costas D Maranas
    Citation: Biotechnology for Biofuels 2014 7:144
  31. Mineral elements present in lignocellulosic biomass feedstocks may accumulate in biorefinery process streams and cause technological problems, or alternatively can be reaped for value addition. A better unders...

    Authors: Duy Michael Le, Hanne R Sørensen, Niels Ole Knudsen, Jan K Schjoerring and Anne S Meyer
    Citation: Biotechnology for Biofuels 2014 7:141
  32. A major obstacle, and perhaps the most important economic barrier to the effective use of plant biomass for the production of fuels, chemicals, and bioproducts, is our current lack of knowledge of how to effic...

    Authors: Daehwan Chung, Sivakumar Pattathil, Ajaya K Biswal, Michael G Hahn, Debra Mohnen and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:147
  33. Enzymatic hydrolysis of pretreated lignocellulosic biomass is an essential process for the production of fermentable sugars for industrial use. A better understanding of fungal cellulase systems will provide c...

    Authors: Hiroyuki Inoue, Stephen R Decker, Larry E Taylor, Shinichi Yano and Shigeki Sawayama
    Citation: Biotechnology for Biofuels 2014 7:151
  34. Members of the bacterial genus Caldicellulosiruptor are the most thermophilic cellulolytic organisms described to date, and have the ability to grow on lignocellulosic biomass without conventional pretreatment. D...

    Authors: Jenna Young, Daehwan Chung, Yannick J Bomble, Michael E Himmel and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:142
  35. For the development of lignocellulosic biofuels a common strategy to release hemicellulosic sugars and enhance the enzymatic digestibility of cellulose is the heat pretreatment of biomass with dilute acid. Dur...

    Authors: Ana B Ibáñez and Stefan Bauer
    Citation: Biotechnology for Biofuels 2014 7:145
  36. Enzymatic breakdown of lignocellulosic biomass is a known bottleneck for the production of high-value molecules and biofuels from renewable sources. Filamentous fungi are the predominant natural source of enzy...

    Authors: David Navarro, Marie-Noëlle Rosso, Mireille Haon, Caroline Olivé, Estelle Bonnin, Laurence Lesage-Meessen, Didier Chevret, Pedro M Coutinho, Bernard Henrissat and Jean-Guy Berrin
    Citation: Biotechnology for Biofuels 2014 7:143
  37. The heterotrophic and mixotrophic culture of oleaginous microalgae is a promising process to produce biofuel feedstock due to the advantage of fast growth. Various organic carbons have been explored for this a...

    Authors: Yubin Zheng, Xiaochen Yu, Tingting Li, Xiaochao Xiong and Shulin Chen
    Citation: Biotechnology for Biofuels 2014 7:125
  38. Lignocellulosic biomass is a promising renewable feedstock for biofuel production. Acetate is one of the major inhibitors liberated from hemicelluloses during hydrolysis. An understanding of the toxic effects ...

    Authors: Shihui Yang, Mary Ann Franden, Steven D Brown, Yat-Chen Chou, Philip T Pienkos and Min Zhang
    Citation: Biotechnology for Biofuels 2014 7:140
  39. Biohydrogen production through dark fermentation using organic waste as a substrate has gained increasing attention in recent years, mostly because of the economic advantages of coupling renewable, clean energ...

    Authors: Iulian Zoltan Boboescu, Mariana Ilie, Vasile Daniel Gherman, Ion Mirel, Bernadett Pap, Adina Negrea, Éva Kondorosi, Tibor Bíró and Gergely Maróti
    Citation: Biotechnology for Biofuels 2014 7:139
  40. The bioconversion of cellulose into simple sugars or chemicals has attracted extensive attention in recent decades. The crystal allomorphs of cellulose are key factor affecting cellulose saccharification. Howe...

    Authors: Ting Cui, Jihong Li, Zhipei Yan, Menghui Yu and Shizhong Li
    Citation: Biotechnology for Biofuels 2014 7:134
  41. Genetically customised Saccharomyces cerevisiae that can produce ethanol and additional bio-based chemicals from sustainable agro-industrial feedstocks (for example, residual plant biomass) are of major interest ...

    Authors: Claire M Hull, E Joel Loveridge, Nicola J Rolley, Iain S Donnison, Steven L Kelly and Diane E Kelly
    Citation: Biotechnology for Biofuels 2014 7:133
  42. Members of the thermophilic, anaerobic Gram-positive bacterial genus Caldicellulosiruptor grow optimally at 65 to 78°C and degrade lignocellulosic biomass without conventional pretreatment. Decomposition of compl...

    Authors: Joseph Groom, Daehwan Chung, Jenna Young and Janet Westpheling
    Citation: Biotechnology for Biofuels 2014 7:132
  43. Rut-C30 is a cellulase-hyperproducing Trichoderma reesei strain and, consequently, became the ancestor of most industry strains used in the production of plant cell wall-degrading enzymes, in particular cellulase...

    Authors: Thiago M Mello-de-Sousa, Rita Gorsche, Alice Rassinger, Marcio J Poças-Fonseca, Robert L Mach and Astrid R Mach-Aigner
    Citation: Biotechnology for Biofuels 2014 7:129
  44. The efficiency and cost of current lignocellulosic enzymes still limit the large-scale production of cellulosic ethanol in industry. Residual lignin after pretreatment severely depresses the activity of polysa...

    Authors: Lei Ji, Jinshui Yang, Hua Fan, Yi Yang, Baozhen Li, Xuejian Yu, Ning Zhu and Hongli Yuan
    Citation: Biotechnology for Biofuels 2014 7:130
  45. Enzymatic hydrolysis is a crucial step of biomass conversion into biofuels and different pretreatments have been proposed to improve the process efficiency. Amongst the various factors affecting hydrolysis yie...

    Authors: Jefferson Esquina Tsuchida, Camila Alves Rezende, Rodrigo de Oliveira-Silva, Marisa Aparecida Lima, Marcel Nogueira d’Eurydice, Igor Polikarpov and Tito José Bonagamba
    Citation: Biotechnology for Biofuels 2014 7:127
  46. The expression of biomass-degrading enzymes (such as cellobiohydrolases) in transgenic plants has the potential to reduce the costs of biomass saccharification by providing a source of enzymes to supplement co...

    Authors: Mark D Harrison, Zhanying Zhang, Kylie Shand, Barrie Fong Chong, Jason Nichols, Paul Oeller, Ian M O'Hara, William OS Doherty and James L Dale
    Citation: Biotechnology for Biofuels 2014 7:131
  47. Efficient industrial processes for converting plant lignocellulosic materials into biofuels are a key to global efforts to come up with alternative energy sources to fossil fuels. Novel cellulolytic enzymes ha...

    Authors: Sebastian GA Konietzny, Phillip B Pope, Aaron Weimann and Alice C McHardy
    Citation: Biotechnology for Biofuels 2014 7:124