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  1. Microalgae as a viable biodiesel feedstock show great potential to approach the challenges of energy shortage and environment pollution, but their economic feasibility was seriously hampered by high production...

    Authors: Lin Luo, Hongyu Ren, Xuanyuan Pei, Guojun Xie, Defeng Xing, Yingqi Dai, Nanqi Ren and Bingfeng Liu

    Citation: Biotechnology for Biofuels 2019 12:218

    Content type: Research

    Published on:

  2. 5-Hydroxymethylfurfural (HMF) is a highly valuable platform chemical that can be obtained from plant biomass carbohydrates. HMF can be oxidized to 2,5-furandicarboxylic acid (FDCA), which is used as a renewabl...

    Authors: Ana Serrano, Eva Calviño, Juan Carro, María I. Sánchez-Ruiz, F. Javier Cañada and Angel T. Martínez

    Citation: Biotechnology for Biofuels 2019 12:217

    Content type: Research

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  3. Increasing seed oil content is one of the most important targets for rapeseed (Brassica napus) breeding. However, genetic mechanisms of mature seed oil content in Brassica napus (B. napus) remain little known. To...

    Authors: Zhongchun Xiao, Chao Zhang, Fang Tang, Bo Yang, Liyuan Zhang, Jingsen Liu, Qiang Huo, Shufeng Wang, Shengting Li, Lijuan Wei, Hai Du, Cunmin Qu, Kun Lu, Jiana Li and Nannan Li

    Citation: Biotechnology for Biofuels 2019 12:216

    Content type: Research

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  4. Animal bedding remains an underutilized source of raw material for bioethanol production, despite the economic and environmental benefits of its use. Further research concerning the optimization of the product...

    Authors: Miguel Sanchis-Sebastiá, Borbála Erdei, Krisztina Kovacs, Mats Galbe and Ola Wallberg

    Citation: Biotechnology for Biofuels 2019 12:215

    Content type: Research

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  5. As a renewable and clean energy carrier, the production of biohydrogen from low-value feedstock such as lignocellulose has increasingly garnered interest. The NADH-dependent reduced ferredoxin:NADP+ oxidoreductas...

    Authors: Yang Li, Jialei Hu, Chunyun Qu, Lili Chen, Xiaolong Guo, Hongxin Fu and Jufang Wang

    Citation: Biotechnology for Biofuels 2019 12:214

    Content type: Research

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  6. In this work, three pretreatments under investigation at the DOE Bioenergy Research Centers (BRCs) were subjected to a side-by-side comparison to assess their performance on model bioenergy hardwoods (a eucaly...

    Authors: Aditya Bhalla, Charles M. Cai, Feng Xu, Sandip K. Singh, Namita Bansal, Thanaphong Phongpreecha, Tanmoy Dutta, Cliff E. Foster, Rajeev Kumar, Blake A. Simmons, Seema Singh, Charles E. Wyman, Eric L. Hegg and David B. Hodge

    Citation: Biotechnology for Biofuels 2019 12:213

    Content type: Research

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  7. Lacto-N-neotetraose (LNnT) is one of the important ingredients of human milk oligosaccharides, which can enhance immunity, regulate intestinal bacteria and promote cell maturation.

    Authors: Xiaomin Dong, Nan Li, Zhenmin Liu, Xueqin Lv, Jianghua Li, Guocheng Du, Miao Wang and Long Liu

    Citation: Biotechnology for Biofuels 2019 12:212

    Content type: Research

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  8. S-Adenosylmethionine (SAM) is a critical cofactor involved in many biochemical reactions. However, the low fermentation titer of SAM in methionine-free medium hampers commercial-scale production. The SAM synthe.....

    Authors: Liying Ruan, Lu Li, Dian Zou, Cong Jiang, Zhiyou Wen, Shouwen Chen, Yu Deng and Xuetuan Wei

    Citation: Biotechnology for Biofuels 2019 12:211

    Content type: Research

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  9. Biofuels derived from lignocellulosic biomass are a viable alternative to fossil fuels required for transportation. Following plant biomass pretreatment, the furan derivative furfural is present at concentrati...

    Authors: Daria Feldman, David J. Kowbel, Adi Cohen, N. Louise Glass, Yitzhak Hadar and Oded Yarden

    Citation: Biotechnology for Biofuels 2019 12:210

    Content type: Research

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  10. Schizochytrium sp. is a marine fungus with great potential as an alternative commercial source of lipids rich in polyunsaturated fatty acids (PUFAs). To further increase lipid accumulation in Schizochytrium sp., ...

    Authors: Zhipeng Li, Xueping Ling, Hao Zhou, Tong Meng, Jinjin Zeng, Wei Hang, Yanyan Shi and Ning He

    Citation: Biotechnology for Biofuels 2019 12:209

    Content type: Research

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  11. In anaerobic cellulolytic micro-organisms, cellulolysis results in the action of several cellulases gathered in extracellular multi-enzyme complexes called cellulosomes. Their action releases cellobiose and lo...

    Authors: Nian Liu, Aurélie Fosses, Clara Kampik, Goetz Parsiegla, Yann Denis, Nicolas Vita, Henri-Pierre Fierobe and Stéphanie Perret

    Citation: Biotechnology for Biofuels 2019 12:208

    Content type: Research

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  12. Formate converted from CO2 reduction has great potential as a sustainable feedstock for biological production of biofuels and biochemicals. Nevertheless, utilization of formate for growth and chemical production ...

    Authors: Seung-Jin Kim, Jihee Yoon, Dae-Kyun Im, Yong Hwan Kim and Min-Kyu Oh

    Citation: Biotechnology for Biofuels 2019 12:207

    Content type: Research

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  13. Cellulose-active lytic polysaccharide monooxygenases (LPMOs) secreted by filamentous fungi play a key role in the degradation of recalcitrant lignocellulosic biomass. They can occur as multidomain proteins fus...

    Authors: Amani Chalak, Ana Villares, Celine Moreau, Mireille Haon, Sacha Grisel, Angélina d’Orlando, Isabelle Herpoël-Gimbert, Aurore Labourel, Bernard Cathala and Jean-Guy Berrin

    Citation: Biotechnology for Biofuels 2019 12:206

    Content type: Research

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  14. 2,3-Butanediol (2,3-BDO) is a valuable chemical for industrial applications. Bacteria can produce 2,3-BDO with a high productivity, though most of their classification as pathogens makes them undesirable for t...

    Authors: Ye-Gi Lee and Jin-Ho Seo

    Citation: Biotechnology for Biofuels 2019 12:204

    Content type: Research

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  15. Compared to the oleaginous yeast Yarrowia lipolytica, Trichosporon cutaneum can metabolize pentose sugars more efficiently, and in the meantime is more tolerant to inhibitors, which is suitable for lipid producti...

    Authors: Ya Wang, Riming Yan, Lijuan Tang, Libin Zhu, Du Zhu and Fengwu Bai

    Citation: Biotechnology for Biofuels 2019 12:203

    Content type: Research

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  16. Oleaginous yeasts are potent hosts for the renewable production of lipids and harbor great potential for derived products, such as biofuels. Several promising processes have been described that produce hydroca...

    Authors: Stefan Bruder, Eva Johanna Moldenhauer, Robert Denis Lemke, Rodrigo Ledesma-Amaro and Johannes Kabisch

    Citation: Biotechnology for Biofuels 2019 12:202

    Content type: Research

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  17. Consolidated bioprocessing (CBP) combines enzyme production, saccharification and fermentation into a one-step process. This strategy represents a promising alternative for economic ethanol production from sta...

    Authors: Rosemary A. Cripwell, Shaunita H. Rose, Lorenzo Favaro and Willem H. van Zyl

    Citation: Biotechnology for Biofuels 2019 12:201

    Content type: Research

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  18. Filamentous fungi secret hydrolytic enzymes like cellulase and hemicellulase outside the cells, serving as important scavengers of plant biomass in nature and workhorses in the enzyme industry. Unlike the exte...

    Authors: Chengcheng Li, Ai-Ping Pang, Hang Yang, Roujing Lv, Zhihua Zhou, Fu-Gen Wu and Fengming Lin

    Citation: Biotechnology for Biofuels 2019 12:200

    Content type: Research

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  19. A consolidated bioprocessing (CBP), where lignocellulose is converted into the desired product(s) in a single fermentative step without the addition of expensive degradative enzymes, represents the ideal solut...

    Authors: Zeenat Bashir, Lili Sheng, Annamma Anil, Arvind Lali, Nigel P. Minton and Ying Zhang

    Citation: Biotechnology for Biofuels 2019 12:199

    Content type: Research

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  20. Enzymatic hydrolysis of lignocellulose by fungi is a key step in global carbon cycle and biomass utilization. Cellulolytic enzyme production is tightly controlled at a transcriptional level. Here, we investiga...

    Authors: Yanan Li, Yueyan Hu, Zhu Zhu, Kaili Zhao, Guodong Liu, Lushan Wang, Yinbo Qu, Jian Zhao and Yuqi Qin

    Citation: Biotechnology for Biofuels 2019 12:198

    Content type: Research

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  21. Layered double hydroxides (LDHs) have received widespread attention for their potential applications in catalysis, polymer nanocomposites, pharmaceuticals, and sensors. Here, the mechanism underlying the physi...

    Authors: Wei Ren, Yanshuai Ding, Lide Gu, Wanli Yan, Cang Wang, Mingsheng Lyu, Changhai Wang and Shujun Wang

    Citation: Biotechnology for Biofuels 2019 12:196

    Content type: Research

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  22. Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process...

    Authors: Pei Liu, Guoxiu Zhang, Yumeng Chen, Jian Zhao, Wei Wang and Dongzhi Wei

    Citation: Biotechnology for Biofuels 2019 12:195

    Content type: Research

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  23. Black soldier fly (BSF, Hermetia illucens L.) can efficiently degrade organic wastes and transform into a high fat containing insect biomass that could be used as feedstock for biodiesel production. Meanwhile, th...

    Authors: Zhaolu Zhu, Kashif ur Rehman, Yongqiang Yu, Xiu Liu, Hui Wang, Jeffery K. Tomberlin, Sing-Hoi Sze, Minmin Cai, Jibin Zhang, Ziniu Yu, Jinshui Zheng and Longyu Zheng

    Citation: Biotechnology for Biofuels 2019 12:194

    Content type: Research

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  24. Switchgrass (Panicum virgatum L.), a warm-season perennial C4 plant, can be used as a forage plant, a soil and water conservation plant, a windbreak plant, and as a good source of biofuels and alternative energy ...

    Authors: Shumeng Zhang, Fengli Sun, Weiwei Wang, Guoyu Yang, Chao Zhang, Yongfeng Wang, Shudong Liu and Yajun Xi

    Citation: Biotechnology for Biofuels 2019 12:193

    Content type: Research

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  25. The current extraction and use of fossil fuels has been linked to extensive negative health and environmental outcomes. Lignocellulosic biomass-derived biofuels and bioproducts are being actively considered as...

    Authors: Winston E. Anthony, Rhiannon R. Carr, Drew M. DeLorenzo, Tayte P. Campbell, Zeyu Shang, Marcus Foston, Tae Seok Moon and Gautam Dantas

    Citation: Biotechnology for Biofuels 2019 12:192

    Content type: Review

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  26. Microalgae are one of the promising feedstock that consists of high carbohydrate content which can be converted into bioethanol. Pre-treatment is one of the critical steps required to release fermentable sugar...

    Authors: Chai Kee Phwan, Kit Wayne Chew, Abdi Hanra Sebayang, Hwai Chyuan Ong, Tau Chuan Ling, Marlinda Abdul Malek, Yeek-Chia Ho and Pau Loke Show

    Citation: Biotechnology for Biofuels 2019 12:191

    Content type: Research

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  27. Xylan is the major component of hemicelluloses, which are the second most abundant polysaccharides in nature, accounting for approximately one-third of all renewable organic carbon resources on earth. Efficien...

    Authors: Xueqiang Liu, Zhengqiang Jiang, Yu Liu, Xin You, Shaoqing Yang and Qiaojuan Yan

    Citation: Biotechnology for Biofuels 2019 12:190

    Content type: Research

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  28. Prehydrolyzate, which is from the prehydrolysis process in dissolving pulps industry, contains various sugar-derived and lignin compounds such as xylooligosaccharides (XOS), gluco-oligosaccharides, xylose, glu...

    Authors: Caoxing Huang, Xucai Wang, Chen Liang, Xiao Jiang, Gan Yang, Jie Xu and Qiang Yong

    Citation: Biotechnology for Biofuels 2019 12:189

    Content type: Research

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  29. Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environme...

    Authors: Ho Myeong Kim, In Seong Choi, Seoyoun Lee, In Min Hwang, Ho Hyun Chun, Seung Gon Wi, Jin-Cheol Kim, Tae Young Shin, Jong Cheol Kim, Jae Su Kim, Junheon Kim and Hae Woong Park

    Citation: Biotechnology for Biofuels 2019 12:188

    Content type: Research

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  30. Yarrowia lipolytica, an oleaginous yeast, is a promising platform strain for production of biofuels and oleochemicals as it can accumulate a high level of lipids in response to nitrogen limitation. Accordingly, m...

    Authors: Minsuk Kim, Beom Gi Park, Eun-Jung Kim, Joonwon Kim and Byung-Gee Kim

    Citation: Biotechnology for Biofuels 2019 12:187

    Content type: Research

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  31. Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for ...

    Authors: Liang Tian, Peter M. Conway, Nicholas D. Cervenka, Jingxuan Cui, Marybeth Maloney, Daniel G. Olson and Lee R. Lynd

    Citation: Biotechnology for Biofuels 2019 12:186

    Content type: Research

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  32. Efforts to develop efficient lignocellulose-degrading enzymatic preparations have led to the relatively recent discovery of a new class of novel cellulase boosters, termed lytic polysaccharide monoxygenases (L...

    Authors: Pooja Dixit, Biswajit Basu, Munish Puri, Deepak Kumar Tuli, Anshu Shankar Mathur and Colin James Barrow

    Citation: Biotechnology for Biofuels 2019 12:185

    Content type: Research

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  33. Microalgal starch is regarded as a promising alternative to crop-based starch for biorefinery such as the production of biofuels and bio-based chemicals. The single or separate use of inorganic carbon source, ...

    Authors: Man Qi, Changhong Yao, Binhuan Sun, Xupeng Cao, Qiang Fei, Bobo Liang, Wenyi Ran, Qi Xiang, Yongkui Zhang and Xianqiu Lan

    Citation: Biotechnology for Biofuels 2019 12:184

    Content type: Research

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  34. Efficient biotechnological conversion of lignocellulosic biomass to valuable products, such as transportation biofuels, is ecologically attractive, yet requires substantially improved mechanistic understanding...

    Authors: Zhenxing Ren, Wuxin You, Shasha Wu, Ansgar Poetsch and Chenggang Xu

    Citation: Biotechnology for Biofuels 2019 12:183

    Content type: Research

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  35. Decreasing fossil fuels and its impact on global warming have led to an increasing demand for its replacement by sustainable renewable biofuels. Microalgae may offer a potential feedstock for renewable biofuel...

    Authors: Kashif Mohd Shaikh, Asha Arumugam Nesamma, Malik Zainul Abdin and Pannaga Pavan Jutur

    Citation: Biotechnology for Biofuels 2019 12:182

    Content type: Research

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  36. Dedicated lignocellulosic feedstock from grass crops for biofuel production is extensively increasing. However, the access to fermentable cell wall sugars by carbohydrate degrading enzymes is impeded by lignin...

    Authors: Philippe Le Bris, Yin Wang, Clément Barbereau, Sébastien Antelme, Laurent Cézard, Frédéric Legée, Angelina D’Orlando, Marion Dalmais, Abdelhafid Bendahmane, Mathias Schuetz, Lacey Samuels, Catherine Lapierre and Richard Sibout

    Citation: Biotechnology for Biofuels 2019 12:181

    Content type: Research

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  37. DNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of mic...

    Authors: Shuwen Liu, Haihan Xiao, Fangfang Zhang, Zheng Lu, Yun Zhang, Aihua Deng, Zhongcai Li, Cui Yang and Tingyi Wen

    Citation: Biotechnology for Biofuels 2019 12:180

    Content type: Research

    Published on:

  38. Countercurrent saccharification is a promising way to minimize enzyme loading while obtaining high conversions and product concentrations. However, in countercurrent saccharification experiments, 3–4 months ar...

    Authors: Chao Liang, Chao Gu, M. Nazmul Karim and Mark Holtzapple

    Citation: Biotechnology for Biofuels 2019 12:179

    Content type: Research

    Published on:

  39. Algal harvesting is a major cost which increases biofuel production cost. Algal biofuels are widely studied as third-generation biofuel. However, they are yet not viable because of its high production cost whi...

    Authors: Arghya Bhattacharya, Megha Mathur, Pushpendar Kumar and Anushree Malik

    Citation: Biotechnology for Biofuels 2019 12:178

    Content type: Research

    Published on:

  40. Pretreatment is effective in reducing the natural recalcitrance of plant biomass so polysaccharides in cell walls can be accessed for conversion to sugars. Furthermore, lignocellulosic biomass must typically b...

    Authors: Abhishek S. Patri, Laura McAlister, Charles M. Cai, Rajeev Kumar and Charles E. Wyman

    Citation: Biotechnology for Biofuels 2019 12:177

    Content type: Research

    Published on:

  41. Many studies have been carried out on the growth-modulating efficiency of plants by the colonization of an endophytic fungus Piriformospora indica. However, studies involving the co-culture of alga with endophyti...

    Authors: Vipul Swarup Bhatnagar, Prasun Bandyopadhyay, Girish H. Rajacharya, Sharanya Sarkar, Krishna Mohan Poluri and Shashi Kumar

    Citation: Biotechnology for Biofuels 2019 12:176

    Content type: Research

    Published on:

  42. Valorization of lignin has the potential to significantly improve the economics of lignocellulosic biorefineries. However, its complex structure makes conversion to useful products elusive. One promising appro...

    Authors: Meghan C. Barnhart-Dailey, Dongmei Ye, Dulce C. Hayes, Danae Maes, Casey T. Simoes, Leah Appelhans, Amanda Carroll-Portillo, Michael S. Kent and Jerilyn A. Timlin

    Citation: Biotechnology for Biofuels 2019 12:175

    Content type: Research

    Published on:

  43. The production of glucose from cellulose requires cellulases, which are obtained from decomposing microorganisms such as fungi and bacteria. Among the cellulases, β-glucosidases convert cellobiose to glucose a...

    Authors: Raíza Azevedo, Jéssika Lawall Lopes, Manuel Macedo de Souza, Betania Ferraz Quirino, Letícia Jungmann Cançado and Luis Fernando Marins

    Citation: Biotechnology for Biofuels 2019 12:174

    Content type: Research

    Published on:

  44. Isobutanol is a promising candidate as second-generation biofuel and has several advantages compared to bioethanol. Another benefit of isobutanol is that it is already formed as a by-product in fermentations w...

    Authors: Johannes Wess, Martin Brinek and Eckhard Boles

    Citation: Biotechnology for Biofuels 2019 12:173

    Content type: Research

    Published on:

  45. Plant seed oil is an important bioresource for human food and animal feed, as well as industrial bioproducts. Therefore, increasing oil content in seeds has been one of the primary targets in the breeding prog...

    Authors: Xi Xie, Dauenpen Meesapyodsuk and Xiao Qiu

    Citation: Biotechnology for Biofuels 2019 12:172

    Content type: Research

    Published on:

  46. Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid–AlCl3 catalyzed conversion to hydroxymet...

    Authors: Haibing Yang, Ximing Zhang, Hao Luo, Baoyuan Liu, Tânia M. Shiga, Xu Li, Jeong Im Kim, Peter Rubinelli, Jonathan C. Overton, Varun Subramanyam, Bruce R. Cooper, Huaping Mo, Mahdi M. Abu-Omar, Clint Chapple, Bryon S. Donohoe, Lee Makowski…

    Citation: Biotechnology for Biofuels 2019 12:171

    Content type: Research

    Published on:

  47. Co-pyrolysis of wastes with other feedstock can synergistically improve the rate of biomass decomposition and also help to resolve the issues related to limited availability feedstock. In this regards, synergi...

    Authors: Arun K. Vuppaladadiyam, Hao Liu, Ming Zhao, Abdul F. Soomro, Muhammad Zaki Memon and Valerie Dupont

    Citation: Biotechnology for Biofuels 2019 12:170

    Content type: Research

    Published on:

  48. Lipid extraction for quantification of fat content in oleaginous yeasts often requires strong acids and harmful organic solvents; it is laborious and time-consuming. Therefore, in most cases just endpoint meas...

    Authors: Mikołaj Chmielarz, Sabine Sampels, Johanna Blomqvist, Jule Brandenburg, Frida Wende, Mats Sandgren and Volkmar Passoth

    Citation: Biotechnology for Biofuels 2019 12:169

    Content type: Methodology

    Published on:

  49. H2–ethanol-coproducing bacteria, as primary fermenters, play important roles in the microbiome of bioreactors for bioenergy production from organic wastewater or solid wastes. Ethanoligenens harbinense YUAN-3 is ...

    Authors: Huahua Li, Xiaoxue Mei, Bingfeng Liu, Guojun Xie, Nanqi Ren and Defeng Xing

    Citation: Biotechnology for Biofuels 2019 12:166

    Content type: Research

    Published on:

  50. Escherichia coli has been proved to be one promising platform chassis for the production of various natural products, such as biofuels. Product toxicity is one of the main bottlenecks for achieving maximum produc...

    Authors: Guochao Xu, Anning Wu, Lin Xiao, Ruizhi Han and Ye Ni

    Citation: Biotechnology for Biofuels 2019 12:164

    Content type: Research

    Published on:

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