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  1. Content type: Research

    During inevitable light/dark cycle, lipid productivity of outdoor microalgae photoautotrophic cultivation is lowered by nightly biomass and lipid loss. To minimize, or even reverse the nightly lipid loss, it w...

    Authors: Feng Qi, Haiyan Pei, Ruimin Mu, Guixia Ma, Daoji Wu and Qiang Han

    Citation: Biotechnology for Biofuels 2019 12:151

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  2. Content type: Research

    3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been imple...

    Authors: Christian Arenas-López, Jessica Locker, Diego Orol, Frederik Walter, Tobias Busche, Jörn Kalinowski, Nigel P. Minton, Katalin Kovács and Klaus Winzer

    Citation: Biotechnology for Biofuels 2019 12:150

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  3. Content type: Methodology

    Fungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including...

    Authors: Timothy C. Cairns, Claudia Feurstein, Xiaomei Zheng, Ping Zheng, Jibin Sun and Vera Meyer

    Citation: Biotechnology for Biofuels 2019 12:149

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  4. Content type: Research

    Microbial lipids derived from various lignocellulosic feedstocks have emerged as a promising candidate for the biodiesel industry and a potential substitute for high value-added fats. However, lignocellulosic ...

    Authors: Wenting Zhou, Mou Tang, Tao Zou, Na Peng, Mi Zhao and Zhiwei Gong

    Citation: Biotechnology for Biofuels 2019 12:148

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  5. Content type: Research

    Methane is the primary component of natural gas and biogas. The huge abundance of methane makes it a promising alternative carbon source for industrial biotechnology. Herein, we report diamine compound, putres...

    Authors: Linh Thanh Nguyen and Eun Yeol Lee

    Citation: Biotechnology for Biofuels 2019 12:147

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  6. Content type: Research

    Trichoderma reesei is the most important industrial producer of lignocellulolytic enzymes. These enzymes play an important role in biomass degradation leading to novel applications of this fungus in the biotechno...

    Authors: Renato Graciano de Paula, Amanda Cristina Campos Antoniêto, Karoline Maria Vieira Nogueira, Liliane Fraga Costa Ribeiro, Marina Campos Rocha, Iran Malavazi, Fausto Almeida and Roberto Nascimento Silva

    Citation: Biotechnology for Biofuels 2019 12:146

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  7. Content type: Research

    Bacillus amyloliquefaciens NB is a newly discovered strain, which produces poly-(γ-glutamic acid) (γ-PGA) from raw extracted inulin of Jerusalem artichoke tubers; however, the underlying mechanisms remain unknown...

    Authors: Yibin Qiu, Yifan Zhu, Yijing Zhan, Yatao Zhang, Yuanyuan Sha, Yijing Zhan, Zongqi Xu, Sha Li, Xiaohai Feng and Hong Xu

    Citation: Biotechnology for Biofuels 2019 12:145

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  8. Content type: Research

    The α-l-arabinofuranosidases (α-l-ABFs) are exoenzymes involved in the hydrolysis of α-l-arabinosyl linkages in plant cell wall polysaccharides. They play a crucial role in the degradation of arabinoxylan and ara...

    Authors: Mohamed Mroueh, Marion Aruanno, Romain Borne, Pascale de Philip, Henri-Pierre Fierobe, Chantal Tardif and Sandrine Pagès

    Citation: Biotechnology for Biofuels 2019 12:144

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  9. Content type: Research

    The extremely thermophilic bacterium Caldicellulosiruptor lactoaceticus can degrade and metabolize untreated lignocellulosic biomass containing xylan. The mechanism of the bacterium for degradation of insoluble x...

    Authors: Xiaojing Jia and Yejun Han

    Citation: Biotechnology for Biofuels 2019 12:143

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  10. Content type: Correction

    After the publication of the article [1], it was brought to our attention that the GenBank Accession number for the EF1 is missing in Methods section. Please find the accession number in the erratum below.

    Authors: Wagner Rodrigo de Souza, Thályta Fraga Pacheco, Karoline Estefani Duarte, Bruno Leite Sampaio, Patrícia Abrão de Oliveira Molinari, Polyana Kelly Martins, Thaís Ribeiro Santiago, Eduardo Fernandes Formighieri, Felipe Vinecky, Ana Paula Ribeiro, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayashi, Rowan Andrew Craig Mitchell, Dasciana de Sousa Rodrigues Gambetta and Hugo Bruno Correa Molinari

    Citation: Biotechnology for Biofuels 2019 12:142

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    The original article was published in Biotechnology for Biofuels 2019 12:111

  11. Content type: Research

    Docosahexaenoic acid (DHA, C22:6) and odd-chain fatty acids (OCFAs, C15:0 and C17:0) have attracted great interest, since they have been widely used in food and therapeutic industries, as well as chemical indu...

    Authors: Fangzhong Wang, Yali Bi, Jinjin Diao, Mingming Lv, Jinyu Cui, Lei Chen and Weiwen Zhang

    Citation: Biotechnology for Biofuels 2019 12:141

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  12. Content type: Research

    Oleaginous yeasts are considered as a potential lipid source for food, feed and biofuel production. In order to make the yeast-based lipid production environmentally and economically sustainable, there is a ne...

    Authors: Volha Shapaval, Jule Brandenburg, Johanna Blomqvist, Valeria Tafintseva, Volkmar Passoth, Mats Sandgren and Achim Kohler

    Citation: Biotechnology for Biofuels 2019 12:140

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  13. Content type: Research

    Expression of d-xylose isomerase having high catalytic activity in Saccharomyces cerevisiae (S. cerevisiae) is a prerequisite for efficient and economical production of bioethanol from cellulosic biomass. Althoug...

    Authors: Taisuke Seike, Yosuke Kobayashi, Takehiko Sahara, Satoru Ohgiya, Yoichi Kamagata and Kazuhiro E. Fujimori

    Citation: Biotechnology for Biofuels 2019 12:139

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  14. Content type: Research

    The lignocellulosic biomass feedstocks such as empty fruit bunches (EFBs) prove to be potential renewable resources owing to their abundance, low prices, and high carbohydrate contents. Generally, the conversi...

    Authors: Jae Kyun Kim, Jungwoo Yang, So Young Park, Ju-Hyun Yu and Kyoung Heon Kim

    Citation: Biotechnology for Biofuels 2019 12:138

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  15. Content type: Research

    Rhodotorula toruloides is a promising platform organism for production of lipids from lignocellulosic substrates. Little is known about the metabolic aspects of lipid production from the lignocellolosic sugar xyl...

    Authors: Ievgeniia A. Tiukova, Jule Brandenburg, Johanna Blomqvist, Sabine Sampels, Nils Mikkelsen, Morten Skaugen, Magnus Ø. Arntzen, Jens Nielsen, Mats Sandgren and Eduard J. Kerkhoven

    Citation: Biotechnology for Biofuels 2019 12:137

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  16. Content type: Research

    The biodegradation of chitin is an important part of the carbon and nitrogen cycles in nature. Speeding up the biotransformation of chitin substrates can not only reduce pollution, but also produce high value-...

    Authors: Xiaomeng Sun, Yingjie Li, Zhennan Tian, Yuanchao Qian, Huaiqiang Zhang and Lushan Wang

    Citation: Biotechnology for Biofuels 2019 12:136

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  17. Content type: Research

    Salix spp. are high-productivity crops potentially used for lignocellulosic biofuels such as bioethanol. In general, pretreatment is needed to facilitate the enzymatic depolymerization process. Biomass resistance...

    Authors: Jonas A. Ohlsson, Henrik R. Hallingbäck, Mohamed Jebrane, Anne E. Harman-Ware, Todd Shollenberger, Stephen R. Decker, Mats Sandgren and Ann-Christin Rönnberg-Wästljung

    Citation: Biotechnology for Biofuels 2019 12:135

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  18. Content type: Research

    One of the major problems in the production of lipids for biotechnological purposes using microalgae is maintaining a high productivity of these molecules without reducing cellular biomass. High production rat...

    Authors: Hector Osorio, Carol Jara, Karen Fuenzalida, Emma Rey-Jurado and Mónica Vásquez

    Citation: Biotechnology for Biofuels 2019 12:134

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  19. Content type: Research

    Optimizing the ability of exoelectrogens is a key factor in boosting the overall efficiency of bioelectrochemical systems. In this study, we construct magnetic microbial fuel cells (MFCs) with magnets with dif...

    Authors: Huihui Zhou, Xiaoxue Mei, Bingfeng Liu, Guojun Xie and Defeng Xing

    Citation: Biotechnology for Biofuels 2019 12:133

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  20. Content type: Research

    Chain elongation forms a new platform technology for the circular production of biobased chemicals from renewable carbon and energy sources. This study aimed to develop a continuous methanol-based chain elonga...

    Authors: Sanne M. de Smit, Kasper D. de Leeuw, Cees J. N. Buisman and David P. B. T. B. Strik

    Citation: Biotechnology for Biofuels 2019 12:132

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  21. Content type: Research

    Carbohydrates are major biomass source in fuel-targeted biorefinery. Arthrospira platensis is the largest commercialized microalgae with good environmental tolerance and high biomass production. However, the trad...

    Authors: Qishun Liu, Changhong Yao, Yongxin Sun, Wei Chen, Haidong Tan, Xupeng Cao, Song Xue and Heng Yin

    Citation: Biotechnology for Biofuels 2019 12:131

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  22. Content type: Research

    Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established.

    Authors: Yan Liang, Aymerick Eudes, Sasha Yogiswara, Beibei Jing, Veronica T. Benites, Reo Yamanaka, Clarabelle Cheng-Yue, Edward E. Baidoo, Jenny C. Mortimer, Henrik V. Scheller and Dominique Loqué

    Citation: Biotechnology for Biofuels 2019 12:130

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  23. Content type: Research

    Among the third-generation biodiesel feed stock, oleaginous marine yeasts are the least studied microorganisms for such purpose.

    Authors: Boutheina Bessadok, Andrea Santulli, Thomas Brück and Saloua Sadok

    Citation: Biotechnology for Biofuels 2019 12:129

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    The Correction to this article has been published in Biotechnology for Biofuels 2019 12:152

  24. Content type: Research

    Global demand for energy is on the rise at a time when limited natural resources are fast depleting. To address this issue, microalgal biofuels are being recommended as a renewable and eco-friendly substitute ...

    Authors: Keita Iwasaki, Asuka Kaneko, Yuji Tanaka, Takahiro Ishikawa, Hemanth Noothalapati and Tatsuyuki Yamamoto

    Citation: Biotechnology for Biofuels 2019 12:128

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  25. Content type: Research

    The co-production of single cell oil (SCO) with value-added products could improve the economic viability of industrial SCO production. The newly isolated oleaginous yeast Cryptococcus podzolicus DSM 27192 was ab...

    Authors: Xiujuan Qian, Olga Gorte, Lin Chen, Wenming Zhang, Weiliang Dong, Jiangfeng Ma, Min Jiang, Fengxue Xin and Katrin Ochsenreither

    Citation: Biotechnology for Biofuels 2019 12:127

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  26. Content type: Research

    Fast, complete, and ultimate removal of inhibitory compounds derived from lignocellulose pretreatment is the prerequisite for efficient production of cellulosic ethanol and biochemicals. Biodetoxification is t...

    Authors: Xia Yi, Qiuqiang Gao, Lei Zhang, Xia Wang, Yanqing He, Fengxian Hu, Jian Zhang, Gen Zou, Shihui Yang, Zhihua Zhou and Jie Bao

    Citation: Biotechnology for Biofuels 2019 12:126

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  27. Content type: Research

    Reducing fresh water consumption and nutrient addition will be an effective way to reduce the whole cost of bioethanol production. On the other hand, treatment of biogas slurry derived from anaerobic digestion...

    Authors: Guowei Duan, Bo Wu, Han Qin, Weiting Wang, Qiong Tan, Yonghua Dai, Yao Qin, Furong Tan, Guoquan Hu and Mingxiong He

    Citation: Biotechnology for Biofuels 2019 12:124

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  28. Content type: Research

    During current submerged fermentation for microbial lipid production, the large-scale reactor operations inevitably consume substantial amounts of water and electricity for aeration, stirring, and temperature ...

    Authors: Lei Liu, Jie Song, Yi Li, Ping Li and Hailei Wang

    Citation: Biotechnology for Biofuels 2019 12:123

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  29. Content type: Research

    Chlorophylls play important roles in photosynthesis, and thus are critical for growth and related metabolic pathways in photosynthetic organisms. They are particularly important in microalgae, emerging as the ...

    Authors: Hyun Gi Koh, Nam Kyu Kang, Seungjib Jeon, Sung-Eun Shin, Byeong-ryool Jeong and Yong Keun Chang

    Citation: Biotechnology for Biofuels 2019 12:122

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  30. Content type: Research

    Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid ac...

    Authors: Liang Fu, Qingcheng Li, Ge Yan, Dandan Zhou and John C. Crittenden

    Citation: Biotechnology for Biofuels 2019 12:121

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  31. Content type: Research

    The main representatives of hemicellulose are xylans, usually decorated β-1,4-linked d-xylose polymers, which are hydrolyzed by xylanases. The efficient utilization and complete hydrolysis of xylans necessitate t...

    Authors: Constantinos Katsimpouras, Grigorios Dedes, Nikolaos S. Thomaidis and Evangelos Topakas

    Citation: Biotechnology for Biofuels 2019 12:120

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  32. Content type: Research

    Microalgae are attracting much attention as a promising feedstock for renewable energy production, while simultaneously providing environmental benefits. So far, comparison studies for microalgae selection for...

    Authors: A. Jebali, F. G. Acién, N. Jiménez-Ruiz, C. Gómez, J. M. Fernández-Sevilla, N. Mhiri, F. Karray, S. Sayadi and E. Molina-Grima

    Citation: Biotechnology for Biofuels 2019 12:119

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  33. Content type: Research

    The glucose–methanol–choline (GMC) superfamily is a large and functionally diverse family of oxidoreductases that share a common structural fold. Fungal members of this superfamily that are characterised and r...

    Authors: Leander Sützl, Gabriel Foley, Elizabeth M J Gillam, Mikael Bodén and Dietmar Haltrich

    Citation: Biotechnology for Biofuels 2019 12:118

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  34. Content type: Research

    Lytic polysaccharide monooxygenases (LPMOs) opened a new horizon for biomass deconstruction. They use a redox mechanism not yet fully understood and the range of substrates initially envisaged to be the crysta...

    Authors: Thamy Lívia Ribeiro Corrêa, Atílio Tomazini Júnior, Lúcia Daniela Wolf, Marcos Silveira Buckeridge, Leandro Vieira dos Santos and Mario Tyago Murakami

    Citation: Biotechnology for Biofuels 2019 12:117

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  35. Content type: Research

    Yeast strains that are tolerant to multiple environmental stresses are highly desired for various industrial applications. Despite great efforts in identifying key genes involved in stress tolerance of budding...

    Authors: Ming-Ming Zhang, Liang Xiong, Ya-Jie Tang, Muhammad Aamer Mehmood, Zongbao Kent Zhao, Feng-Wu Bai and Xin-Qing Zhao

    Citation: Biotechnology for Biofuels 2019 12:116

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  36. Content type: Research

    (Pseudo)Bacteroides cellulosolvens is a cellulolytic bacterium that produces the most extensive and intricate cellulosomal system known in nature. Recently, the elaborate architecture of the B. cellulosolvens cel...

    Authors: Olga Zhivin-Nissan, Bareket Dassa, Ely Morag, Meital Kupervaser, Yishai Levin and Edward A. Bayer

    Citation: Biotechnology for Biofuels 2019 12:115

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  37. Content type: Research

    In the present work, the main inhibitors of the yeast cells (vanillin, furfural, formic, and levulinic acid) were generated by pretreatments or hydrolysis (sulfuric acid or enzymes) to convert reducing sugars ...

    Authors: Cecilia Laluce, Longinus I. Igbojionu, José L. Silva and Clóvis A. Ribeiro

    Citation: Biotechnology for Biofuels 2019 12:114

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  38. Content type: Review

    With the increased attention on bio-based industry, demands for techniques that enable fast and effective strain improvement have been dramatically increased. Evolutionary engineering, which is less dependent ...

    Authors: Jina Yang, Beomhee Kim, Gi Yeon Kim, Gyoo Yeol Jung and Sang Woo Seo

    Citation: Biotechnology for Biofuels 2019 12:113

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  39. Content type: Research

    Clostridium spp. can synthesize valuable chemicals and fuels by utilizing diverse waste-stream substrates, including starchy biomass, lignocellulose, and industrial waste gases. However, metabolic engineering in

    Authors: Gabriele Philipps, Sebastian de Vries and Stefan Jennewein

    Citation: Biotechnology for Biofuels 2019 12:112

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  40. Content type: Research

    Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal c...

    Authors: Wagner Rodrigo de Souza, Thályta Fraga Pacheco, Karoline Estefani Duarte, Bruno Leite Sampaio, Patrícia Abrão de Oliveira Molinari, Polyana Kelly Martins, Thaís Ribeiro Santiago, Eduardo Fernandes Formighieri, Felipe Vinecky, Ana Paula Ribeiro, Bárbara Andrade Dias Brito da Cunha, Adilson Kenji Kobayashi, Rowan Andrew Craig Mitchell, Dasciana de Sousa Rodrigues Gambetta and Hugo Bruno Correa Molinari

    Citation: Biotechnology for Biofuels 2019 12:111

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    The Correction to this article has been published in Biotechnology for Biofuels 2019 12:142

  41. Content type: Research

    Lipolytic enzymes of hyperthermophilic archaea generally prefer small carbon chain fatty acid esters (C2–C12) and are categorized as esterases. However, a few have shown activity with long-chain fatty acid esters...

    Authors: Surabhi Soni, Sneha S. Sathe, Rutuja R. Sheth, Prince Tiwari, Rajesh-Kumar N. Vadgama, Annamma Anil Odaneth, Arvind M. Lali and Sanjeev K. Chandrayan

    Citation: Biotechnology for Biofuels 2019 12:110

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  42. Content type: Research

    Grass glucuronoarabinoxylan (GAX) substitutions can inhibit enzymatic degradation and are involved in the interaction of xylan with cell wall cellulose and lignin, factors which contribute to the recalcitrance...

    Authors: Theodora Tryfona, Mathias Sorieul, Carolina Feijao, Katherine Stott, Denis V. Rubtsov, Nadine Anders and Paul Dupree

    Citation: Biotechnology for Biofuels 2019 12:109

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  43. Content type: Research

    Downregulation of genes involved in lignin biosynthesis and related biochemical pathways has been used as a strategy to improve biofuel production. Plant C1 metabolism provides the methyl units used for the me...

    Authors: Hongli Xie, Nancy L. Engle, Sivasankari Venketachalam, Chang Geun Yoo, Jaime Barros, Mitch Lecoultre, Nikki Howard, Guifen Li, Liang Sun, Avinash C. Srivastava, Sivakumar Pattathil, Yunqiao Pu, Michael G. Hahn, Arthur J. Ragauskas, Richard S. Nelson, Richard A. Dixon…

    Citation: Biotechnology for Biofuels 2019 12:108

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  44. Content type: Research

    Due to the intact structure of lignocellulosic biomass, pretreatment was a prerequisite to improve the enzymatic hydrolysis by disrupting the recalcitrant lignocellulose and increasing the accessibility of cel...

    Authors: Hongdan Zhang, Shihang Huang, Weiqi Wei, Jiajie Zhang and Jun Xie

    Citation: Biotechnology for Biofuels 2019 12:107

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  45. Content type: Research

    Monitoring and providing early warning are essential operations in the anaerobic digestion (AD) process. However, there are still several challenges for identifying the early warning indicators and their thres...

    Authors: Yiran Wu, Adam Kovalovszki, Jiahao Pan, Cong Lin, Hongbin Liu, Na Duan and Irini Angelidaki

    Citation: Biotechnology for Biofuels 2019 12:106

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  46. Content type: Research

    Soil ascomycete fungi produce plant-biomass-degrading enzymes to facilitate nutrient and energy uptake in response to exogenous stress. This is controlled by a complex signal network, but the regulatory mechan...

    Authors: Lu-Sheng Liao, Cheng-Xi Li, Feng-Fei Zhang, Yu-Si Yan, Xue-Mei Luo, Shuai Zhao and Jia-Xun Feng

    Citation: Biotechnology for Biofuels 2019 12:105

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  47. Content type: Research

    Commercial biogas upgrading facilities are expensive and consume energy. Biological biogas upgrading may serve as a low-cost approach because it can be easily integrated with existing facilities at biogas plan...

    Authors: Radziah Wahid, Daniel Girma Mulat, John Christian Gaby and Svein Jarle Horn

    Citation: Biotechnology for Biofuels 2019 12:104

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  48. Content type: Research

    Solid-state fermentation (SSF) mimics the natural decay environment of soil fungi and can be employed to investigate the production of plant biomass-degrading enzymes. However, knowledge on the transcriptional...

    Authors: Shuai Zhao, Qi Liu, Jiu-Xiang Wang, Xu-Zhong Liao, Hao Guo, Cheng-Xi Li, Feng-Fei Zhang, Lu-Sheng Liao, Xue-Mei Luo and Jia-Xun Feng

    Citation: Biotechnology for Biofuels 2019 12:103

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