Articles
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Citation: Biotechnology for Biofuels and Bioproducts 2023 16:138
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Ethanol tolerance in engineered strains of Clostridium thermocellum
Clostridium thermocellum is a natively cellulolytic bacterium that is promising candidate for cellulosic biofuel production, and can produce ethanol at high yields (75–80% of theoretical) but the ethanol titers p...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:137 -
Enhancement of hemostatic properties of Cyclotella cryptica frustule through genetic manipulation
The silicified cell wall of diatoms, also known as frustule, shows huge potential as an outstanding bio-nanomaterial for hemostatic applications due to its high hemostatic efficiency, good biocompatibility, an...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:136 -
Metabolic engineering of Thermoanaerobacterium AK17 for increased ethanol production in seaweed hydrolysate
Sustainably produced renewable biomass has the potential to replace fossil-based feedstocks, for generation of biobased fuels and chemicals of industrial interest, in biorefineries. In this context, seaweeds c...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:135 -
Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803
Cyanobacteria are emerging as green cell factories for sustainable biofuel and chemical production, due to their photosynthetic ability to use solar energy, carbon dioxide and water in a direct process. The mo...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:134 -
Engineering a marine microalga Chlorella sp. as the cell factory
The use of marine microalgae in industrial systems is attractive for converting CO2 into value-added products using saline water and sunlight. The plant nature and demonstrated industrial potential facilitate Chl...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:133 -
Functional characterization of fungal lytic polysaccharide monooxygenases for cellulose surface oxidation
Microbial lytic polysaccharide monooxygenases (LPMOs) cleave diverse biomass polysaccharides, including cellulose and hemicelluloses, by initial oxidation at C1 or C4 of glycan chains. Within the Carbohydrate-...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:132 -
Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition
Microalgal biomass represents a sustainable bioresource for various applications, such as food, nutraceuticals, pharmaceuticals, feed, and other bio-based products. For decades, its mass production has attract...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:131 -
Whole genome sequencing and the lignocellulose degradation potential of Bacillus subtilis RLI2019 isolated from the intestine of termites
Lignocellulosic biomass is the most abundant and renewable terrestrial raw material for conversion into bioproducts and biofuels. However, the low utilization efficiency of lignocellulose causes environmental ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:130 -
Remodeling of the 3D chromatin architecture in the marine microalga Nannochloropsis oceanica during lipid accumulation
Genomic three-dimensional (3D) spatial organization plays a key role in shaping gene expression and associated chromatin modification, and it is highly sensitive to environmental stress conditions. In microalg...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:129 -
Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae
Betalains, comprising red–violet betacyanins and yellow–orange betaxanthins, are the hydrophilic vacuolar pigments that provide bright coloration to roots, fruits, and flowers of plants of the Caryophyllales o...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:128 -
Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase
β-Carotene is one of the economically important carotenoids, having functions as the antioxidant to remove harmful free radicals and as the precursor for vitamin A and other high-valued xanthophyll such as zea...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:127 -
Glycolic acid-based deep eutectic solvents boosting co-production of xylo-oligomers and fermentable sugars from corncob and the related kinetic mechanism
Xylo-oligomers are a kind of high value-added products in biomass fractionation. Although there are several chemical methods to obtain xylo-oligomers from biomass, the reports about the deep eutectic solvents ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:126 -
Cellulase–lactic acid bacteria synergy action regulates silage fermentation of woody plant
Feed shortage is an important factor limiting livestock production in the world. To effectively utilize natural woody plant resources, we used wilting and microbial additives to prepare an anaerobic fermentati...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:125 -
Switching carbon metabolic flux for enhancing the production of sesquiterpene-based high-density biofuel precursor in Saccharomyces cerevisiae
Sesquiterpenes are designated as a large class of plant-derived natural active compounds, which have wide applications in industries of energy, food, cosmetics, medicine and agriculture. Neither plant extracti...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:124 -
Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production
Alanine dehydrogenase (AlaDH) belongs to oxidoreductases, and it exists in several different bacteria species and plays a key role in microbial carbon and nitrogen metabolism, spore formation and photosynthesi...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:123 -
A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis
ATP, an important cofactor, is involved in many biocatalytic reactions that require energy. Polyphosphate kinases (PPK) can provide energy for ATP-consuming reactions due to their cheap and readily available s...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:122 -
Unravelling and engineering an operon involved in the side-chain degradation of sterols in Mycolicibacterium neoaurum for the production of steroid synthons
Harnessing engineered Mycolicibacteria to convert cheap phytosterols into valuable steroid synthons is a basic way in the industry for the production of steroid hormones. Thus, C-19 and C-22 steroids are the two ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:121 -
Correction: Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:120 -
Engineering Saccharomyces cerevisiae for improved biofilm formation and ethanol production in continuous fermentation
Biofilm-immobilized continuous fermentation has the potential to enhance cellular environmental tolerance, maintain cell activity and improve production efficiency.
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:119 -
Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei
Induction of cellulase in cellulolytic fungi Trichoderma reesei is strongly activated by cellulosic carbon sources. The transport of cellulosic inducer and the perception of inducing signal is generally considere...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:118 -
Integrated transcriptomic and metabolomic analysis reveals the effects of polyploidization on the lignin content and metabolic pathway in Eucalyptus
Lignin is a major restriction factor for the industrial production of biomass resources, such as pulp and bioenergy. Eucalyptus is one of the most important sources of pulp and bioenergy. After polyploidization, ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:117 -
Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate
Fatty acid-derived products such as fatty alcohols (FAL) find growing application in cosmetic products, lubricants, or biofuels. So far, FAL are primarily produced petrochemically or through chemical conversio...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:116 -
Engineered Escherichia coli platforms for tyrosine-derivative production from phenylalanine using phenylalanine hydroxylase and tetrahydrobiopterin-regeneration system
Aromatic compounds derived from tyrosine are important and diverse chemicals that have industrial and commercial applications. Although these aromatic compounds can be obtained by extraction from natural produ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:115 -
Regulation of acetate tolerance by small ORF-encoded polypeptides modulating efflux pump specificity in Methylomonas sp. DH-1
Methanotrophs have emerged as promising hosts for the biological conversion of methane into value-added chemicals, including various organic acids. Understanding the mechanisms of acid tolerance is essential f...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:114 -
Transcriptional insights into Chlorella sp. ABC-001: a comparative study of carbon fixation and lipid synthesis under different CO2 conditions
Microalgae's low tolerance to high CO2 concentrations presents a significant challenge for its industrial application, especially when considering the utilization of industrial exhaust gas streams with high CO2 c...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:113 -
Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles
The efficient production of sustainable biofuels is important for the reduction of greenhouse gas emissions. Clostridium thermocellum ATCC 27405 is a candidate for ethanol production from lignocellulosic biomass ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:112 -
Plug-flow hydrolysis with lignocellulosic residues: effect of hydraulic retention time and thin-sludge recirculation
Two parallel plug-flow reactors were successfully applied as a hydrolysis stage for the anaerobic pre-digestion of maize silage and recalcitrant bedding straw (30% and 66% w/w) under variations of the hydrauli...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:111 -
Optimization of biogas production from anaerobic co-digestion of fish waste and water hyacinth
Many fresh water bodies face a great challenge of an invasive weed called water hyacinth (WH) which has great impacts on the environment, ecology, and society. Food and Agriculture Organization (FAO) estimates...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:110 -
Biosynthesis pathways of expanding carbon chains for producing advanced biofuels
Because the thermodynamic property is closer to gasoline, advanced biofuels (C ≥ 6) are appealing for replacing non-renewable fossil fuels using biosynthesis method that has presented a promising approach. Syn...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:109 -
Correction: Systems metabolic engineering of Escherichia coli for hyper-production of 5-aminolevulinic acid
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:108 -
Efficient production of icariin and baohuoside I from Epimedium Folium flavonoids by fungal α-l-rhamnosidase hydrolysing regioselectively the terminal rhamnose of epimedin C
Industrial application of icariin and baohuoside I has been hindered by the short supply to a great extent. In this work, a novel GH78 α-l-rhamnosidase AmRha catalyzed the bioconversion of low-value epimedin C in...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:107 -
Stem-loop structures control mRNA processing of the cellulosomal cip-cel operon in Ruminiclostridium cellulolyticum
Anaerobic, mesophilic, and cellulolytic Ruminiclostridium cellulolyticum produces an efficient cellulolytic extracellular complex named cellulosome, which consist of a non-catalytic multi-functional integrating s...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:106 -
The combined effect of EGR and hydrogen addition on a Syzygium cumini (jamun) liquid biofuel engine
Rapid depletion of fossil fuels required the development of alternate and sustainable fuel sources that could replace conventional fuel while having no negative environmental impact. Combining hydrogen inducti...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:105 -
Folate-mediated one-carbon metabolism as a potential antifungal target for the sustainable cultivation of microalga Haematococcus pluvialis
Microalgae are widely considered as multifunctional cell factories that are able to transform the photo-synthetically fixed CO2 to numerous high-value compounds, including lipids, carbohydrates, proteins and pigm...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:104 -
Physiochemical and molecular responses of the diatom Phaeodactylum tricornutum to illumination transitions
Light is a key regulatory factor for photosynthesis and metabolism of microalgae. The diatom Phaeodactylum tricornutum is capable of exhibiting metabolic flexibility in response to light fluctuations. However, th...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:103 -
Cold plasma pretreatment reinforces the lignocellulose-derived aldehyde inhibitors tolerance and bioethanol fermentability for Zymomonas mobilis
Lignocellulose-derived aldehyde inhibitors seriously blocked the biorefinery of biofuels and biochemicals. To date, the economic production of lignocellulose-based products heavily relied on high productivitie...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:102 -
Multivariate modular metabolic engineering for enhanced l-methionine biosynthesis in Escherichia coli
l-Methionine is the only bulk amino acid that has not been industrially produced by the fermentation method. Due to highly complex and strictly regulated biosynthesis, the development of microbial strains for h.....
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:101 -
Systematic approaches to C-lignin engineering in Medicago truncatula
C-lignin is a homopolymer of caffeyl alcohol present in the seed coats of a variety of plant species including vanilla orchid, various cacti, and the ornamental plant Cleome hassleriana. Because of its unique che...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:100 -
Valorization of Boehmeria nivea stalk towards multipurpose fractionation: furfural, pulp, and phenolic monomers
As one of the most abundant bioresource in nature, the value-added utilization of lignocellulosic biomass is limited due to its inherent stubbornness. Pretreatment is a necessary step to break down the recalci...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:99 -
Driving the conversion of phytosterol to 9α-hydroxy-4-androstene-3,17-dione in Mycolicibacterium neoaurum by engineering the supply and regeneration of flavin adenine dinucleotide
The conversion of phytosterols to steroid synthons by engineered Mycolicibacteria comprises one of the core steps in the commercial production of steroid hormones. This is a complex oxidative catabolic process, a...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:98 -
Green construction and release mechanism of lignin-based double-layer coated urea
Lignin played an important role in the establishment of coated fertilizers coating material as a substitute for petrochemical raw materials. However, so far, the lignin-based coated fertilizers was limited in ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:97 -
Production of short-chain fatty acids (SCFAs) as chemicals or substrates for microbes to obtain biochemicals
Carboxylic acids have become interesting platform molecules in the last years due to their versatility to act as carbon sources for different microorganisms or as precursors for the chemical industry. Among ca...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:96 -
Regression modelling of conditional morphogene expression links and quantifies the impact of growth rate, fitness and macromorphology with protein secretion in Aspergillus niger
Filamentous fungi are used as industrial cell factories to produce a diverse portfolio of proteins, organic acids, and secondary metabolites in submerged fermentation. Generating optimized strains for maximum ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:95 -
One-pot synthesis of fuel precursor from acetoin fermentation broth using ionic liquid-based salting-out extraction system
The development of biofuels, especially liquid hydrocarbon fuels, has been widely concerned due to the depletion of fossil resources. In order to obtain fuel precursors, the reaction of C–C bond formation is u...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:94 -
Alcohol acyltransferases for the biosynthesis of esters
Esters are widely used in food, energy, spices, chemical industry, etc., becoming an indispensable part of life. However, their production heavily relies on the fossil energy industry, which presents significa...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:93 -
Multi-step biosynthesis of the biodegradable polyester monomer 2-pyrone-4,6-dicarboxylic acid from glucose
2-Pyrone-4,6-dicarboxylic acid (PDC), a chemically stable pseudoaromatic dicarboxylic acid, represents a promising building block for the manufacture of biodegradable polyesters. Microbial production of PDC ha...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:92 -
The metabolome and bacterial composition of high-moisture Italian ryegrass silage inoculated with lactic acid bacteria during ensiling
With its high nutritional value and productivity, Italian ryegrass as a biomass feedstock constantly supplies rumen degradable nitrogen and digestible fiber to ruminants. However, biofuel production is easily ...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:91 -
Improved bioenergy value of residual rice straw by increased lipid levels from upregulation of fatty acid biosynthesis
Rice (Oryza sativa) straw is a common waste product that represents a considerable amount of bound energy. This energy can be used for biogas production, but the rate and level of methane produced from rice straw...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:90 -
Heterologous expression and characterization of novel GH12 β-glucanase and AA10 lytic polysaccharide monooxygenase from Streptomyces megaspores and their synergistic action in cellulose saccharification
The combination of cellulase and lytic polysaccharide monooxygenase (LPMO) is known to boost enzymatic saccharification of cellulose. Although the synergy between cellulases (GH5, 6 or 7) and LPMOs (AA9) has b...
Citation: Biotechnology for Biofuels and Bioproducts 2023 16:89