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  1. Content type: Research

    Liquid hot water (LHW) pretreatment has been considered as one of the most industrially viable and environment-friendly methods for facilitating the transformation of lignocelluloses into biofuels through biol...

    Authors: Xiaolin Luo, Jing Liu, Peitao Zheng, Meng Li, Yang Zhou, Liulian Huang, Lihui Chen and Li Shuai

    Citation: Biotechnology for Biofuels 2019 12:51

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  2. Content type: Research

    Inefficient utilization of glycerol by Clostridium beijerinckii (Cb) is a major impediment to adopting glycerol metabolism as a strategy for increasing NAD(P)H regeneration, which would in turn, alleviate the tox...

    Authors: Chidozie Victor Agu, Victor Ujor and Thaddeus Chukwuemeka Ezeji

    Citation: Biotechnology for Biofuels 2019 12:50

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  3. Content type: Research

    The thermotolerant yeast Kluyveromyces marxianus is a potential candidate for high-temperature fermentation. When K. marxianus was used for high-temperature ethanol fermentation, a fermentation arrest was observe...

    Authors: Pengsong Li, Xiaofen Fu, Ming Chen, Lei Zhang and Shizhong Li

    Citation: Biotechnology for Biofuels 2019 12:49

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  4. Content type: Research

    Cellulose and hemicellulose are the two largest components in lignocellulosic biomass. Enzymes with activities towards cellulose and xylan have attracted great interest in the bioconversion of lignocellulosic ...

    Authors: Kui Wang, Ruoting Cao, Meiling Wang, Qibin Lin, Ruoting Zhan, Hui Xu and Sidi Wang

    Citation: Biotechnology for Biofuels 2019 12:48

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  5. Content type: Research

    Beer is the most popular alcoholic beverage worldwide. In the manufacture of beer, various by-products and residues are generated, and the most abundant (85% of total by-products) are spent grains. Thanks to i...

    Authors: Simona Giacobbe, Alessandra Piscitelli, Francesca Raganati, Vincenzo Lettera, Giovanni Sannia, Antonio Marzocchella and Cinzia Pezzella

    Citation: Biotechnology for Biofuels 2019 12:47

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  6. Content type: Research

    Microbial fuel cells (MFCs) are effective biofuel devices that use indigenous microbes to directly convert chemical energy from organics oxidation into bioelectric energy. To maximize energy-converting efficie...

    Authors: Bin Xu, John Chiwei Lan, Qingjiang Sun, Chungchuan Hsueh and Bor-Yann Chen

    Citation: Biotechnology for Biofuels 2019 12:46

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  7. Content type: Research

    Lignocellulosic biomass is an abundant and sustainable feedstock, which represents a promising raw material for the production of lactic acid via microbial fermentation. However, toxic compounds that affect mi...

    Authors: Martina Aulitto, Salvatore Fusco, David Benjamin Nickel, Simonetta Bartolucci, Patrizia Contursi and Carl Johan Franzén

    Citation: Biotechnology for Biofuels 2019 12:45

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  8. Content type: Research

    Renewable energy has become a field of high interest over the past decade, and production of biofuels from cellulosic substrates has a particularly high potential as an alternative source of energy. Industrial...

    Authors: Amaranta Kahn, Sarah Moraïs, Anastasia P. Galanopoulou, Daehwan Chung, Nicholas S. Sarai, Neal Hengge, Dimitris G. Hatzinikolaou, Michael E. Himmel, Yannick J. Bomble and Edward A. Bayer

    Citation: Biotechnology for Biofuels 2019 12:44

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  9. Content type: Research

    Cyanobacteria are ideal model organisms to exploit photosynthetically derived electrons or fixed carbon for the biotechnological synthesis of high value compounds and energy carriers. Much effort is spent on t...

    Authors: Marcel Grund, Torsten Jakob, Christian Wilhelm, Bruno Bühler and Andreas Schmid

    Citation: Biotechnology for Biofuels 2019 12:43

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  10. Content type: Research

    Polysaccharide monooxygenases (PMOs) play an important role in the enzymatic degradation of cellulose. They have been demonstrated to able to C6-oxidize cellulose to produce C6-hexodialdoses. However, the biol...

    Authors: Jinyin Chen, Xiuna Guo, Min Zhu, Chen Chen and Duochuan Li

    Citation: Biotechnology for Biofuels 2019 12:42

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  11. Content type: Research

    Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an ...

    Authors: Yusuke Sasaki, Thomas Eng, Robin A. Herbert, Jessica Trinh, Yan Chen, Alberto Rodriguez, John Gladden, Blake A. Simmons, Christopher J. Petzold and Aindrila Mukhopadhyay

    Citation: Biotechnology for Biofuels 2019 12:41

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  12. Content type: Research

    Lignocellulosic hydrolysates contain a mixture of hexose (C6)/pentose (C5) sugars and pretreatment-generated inhibitors (furans, weak acids and phenolics). Therefore, robust yeast isolates with characteristics...

    Authors: Ajay Kumar Pandey, Mohit Kumar, Sonam Kumari, Priya Kumari, Farnaz Yusuf, Shaik Jakeer, Sumera Naz, Piyush Chandna, Ishita Bhatnagar and Naseem A. Gaur

    Citation: Biotechnology for Biofuels 2019 12:40

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  13. Content type: Research

    Light/dark cycling is an inevitable outdoor culture condition for microalgal biofuel production; however, the influence of this cycling on cellular lipid production has not been clearly established. The genera...

    Authors: Yuichi Kato, Yusuke Fujihara, Christopher J. Vavricka, Jo-Shu Chang, Tomohisa Hasunuma and Akihiko Kondo

    Citation: Biotechnology for Biofuels 2019 12:39

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  14. Content type: Research

    Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis. Translational pausing coordinates protein translation and co...

    Authors: Xihao Liao, Jing Zhao, Shuli Liang, Jingjie Jin, Cheng Li, Ruiming Xiao, Lu Li, Meijin Guo, Gong Zhang and Ying Lin

    Citation: Biotechnology for Biofuels 2019 12:38

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  15. Content type: Research

     Lignocellulosic biomass is considered as a potential source for sustainable biofuels. In the conversion process, a pretreatment step is necessary in order to overcome the biomass recalcitrance and allow for s...

    Authors: Christoph-Maximilian Seidel, Simone Brethauer, László Gyenge, Philipp Rudolf von Rohr and Michael H. Studer

    Citation: Biotechnology for Biofuels 2019 12:37

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  16. Content type: Research

    The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large q...

    Authors: Yumeng Chen, Chuan Wu, Yaling Shen, Yushu Ma, Dongzhi Wei and Wei Wang

    Citation: Biotechnology for Biofuels 2019 12:36

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  17. Content type: Research

    The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for ...

    Authors: Shiyue Liu, Ya-Jun Liu, Yingang Feng, Bin Li and Qiu Cui

    Citation: Biotechnology for Biofuels 2019 12:35

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  18. Content type: Research

    Enzymatic hydrolysis is a major step for cellulosic ethanol production. A thorough understanding of enzymatic hydrolysis is necessary to help design optimal conditions and economical systems. The original HCH-...

    Authors: Chao Liang, Chao Gu, Jonathan Raftery, M. Nazmul Karim and Mark Holtzapple

    Citation: Biotechnology for Biofuels 2019 12:34

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  19. Content type: Research

    This review presents a critical assessment of emerging microfluidic technologies for the application on biological productions of biofuels and other chemicals from microalgae. Comparisons of cell culture desig...

    Authors: Pierre Bodénès, Hsiang-Yu Wang, Tsung-Hua Lee, Hung-Yu Chen and Chun-Yen Wang

    Citation: Biotechnology for Biofuels 2019 12:33

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  20. Content type: Review

    Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorizati...

    Authors: Zhaoxian Xu, Peng Lei, Rui Zhai, Zhiqiang Wen and Mingjie Jin

    Citation: Biotechnology for Biofuels 2019 12:32

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  21. Content type: Research

    Clostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and eth...

    Authors: Celine Foulquier, Ching-Ning Huang, Ngoc-Phuong-Thao Nguyen, Axel Thiel, Tom Wilding-Steel, Julie Soula, Minyeong Yoo, Armin Ehrenreich, Isabelle Meynial-Salles, Wolfgang Liebl and Philippe Soucaille

    Citation: Biotechnology for Biofuels 2019 12:31

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  22. Content type: Research

    Biofilms with immobilized cells encased in extracellular polymeric substance are beneficial for industrial fermentation. Their formation is regulated by various factors, including nitric oxide (NO), which is r...

    Authors: Leyun Yang, Cheng Zheng, Yong Chen, Xinchi Shi, Zhuojun Ying and Hanjie Ying

    Citation: Biotechnology for Biofuels 2019 12:30

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  23. Content type: Research

    Bioprocessing offers a sustainable and green approach to manufacture various chemicals and materials. Development of bioprocesses requires transforming common producer strains to cell factories. 13C metabolic flu...

    Authors: Ruilian Yao, Jiawei Li, Lei Feng, Xuehong Zhang and Hongbo Hu

    Citation: Biotechnology for Biofuels 2019 12:29

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  24. Content type: Research

    The green alga Chlorella zofingiensis has been recognized as an industrially relevant strain because of its robust growth under multiple trophic conditions and the potential for simultaneous production of triacyl...

    Authors: Xuemei Mao, Tao Wu, Yaping Kou, Ying Shi, Yu Zhang and Jin Liu

    Citation: Biotechnology for Biofuels 2019 12:28

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  25. Content type: Research

    Microalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more eff...

    Authors: Xiaobin Wen, Huanping Tao, Xinan Peng, Zhongjie Wang, Yi Ding, Yan Xu, Lin Liang, Kui Du, Aoqi Zhang, Caixia Liu, Yahong Geng and Yeguang Li

    Citation: Biotechnology for Biofuels 2019 12:27

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  26. Content type: Research

    Substrate spectra for anaerobic digestion have been broadened in the past decade, inter alia, due to the application of different pretreatment strategies and now include materials rich in lignocellulose, prote...

    Authors: Andreas Otto Wagner, Eva Maria Prem, Rudolf Markt, Rüdiger Kaufmann and Paul Illmer

    Citation: Biotechnology for Biofuels 2019 12:26

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  27. Content type: Correction

    Following publication of the original article [1], the authors reported that the omission of author name.

    Authors: Evert K. Holwerda, Robert S. Worthen, Ninad Kothari, Ronald C. Lasky, Brian H. Davison, Chunxiang Fu, Zeng-Yu Wang, Richard A. Dixon, Ajaya K. Biswal, Debra Mohnen, Richard S. Nelson, Holly L. Baxter, Mitra Mazarei, C. Neal Stewart Jr., Wellington Muchero, Gerald A. Tuskan…

    Citation: Biotechnology for Biofuels 2019 12:25

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    The original article was published in Biotechnology for Biofuels 2019 12:15

  28. Content type: Research

    The production of biohydrogen (H2) as a promising future fuel in anaerobic hyperthermophiles has attracted great attention because H2 formation is more thermodynamically feasible at elevated temperatures and fewe...

    Authors: Seong Hyuk Lee, Min-Sik Kim, Sung Gyun Kang and Hyun Sook Lee

    Citation: Biotechnology for Biofuels 2019 12:24

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  29. Content type: Research

    Due to its wide range of applications in the food, pharmaceutical and chemical fields, microbial synthesis of succinic acid is receiving growing attention, generating already relevant industrial results, as we...

    Authors: Donatella Cimini, Lucio Zaccariello, Sergio D’Ambrosio, Licia Lama, Giovanna Ruoppolo, Olimpia Pepe, Vincenza Faraco and Chiara Schiraldi

    Citation: Biotechnology for Biofuels 2019 12:22

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  30. Content type: Research

    Nannochloropsis oceanica belongs to a large group of photoautotrophic eukaryotic organisms that play important roles in fixation and cycling of atmospheric CO2. Its capability of storing solar energy and carbon d...

    Authors: Chaoyun Chen, Andreas Harst, Wuxin You, Jian Xu, Kang Ning and Ansgar Poetsch

    Citation: Biotechnology for Biofuels 2019 12:21

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  31. Content type: Research

    Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlen...

    Authors: Joana T. Cunha, Pedro O. Soares, Aloia Romaní, Johan M. Thevelein and Lucília Domingues

    Citation: Biotechnology for Biofuels 2019 12:20

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  32. Content type: Research

    Recently, we showed that steady-state continuous cultures of S. stipitis follow the principles of growth on mixture of two complementary substrates. More precisely, when such cultures are fed with progressively h...

    Authors: Shraddha Maitra and Atul Narang

    Citation: Biotechnology for Biofuels 2019 12:19

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  33. Content type: Research

    Among all organisms tested, Haematococcus pluvialis can accumulate the highest levels of natural astaxanthin. Nitrogen starvation and high irradiance promote the accumulation of starch, lipid, and astaxanthin in

    Authors: Luodong Huang, Baoyan Gao, Manman Wu, Feifei Wang and Chengwu Zhang

    Citation: Biotechnology for Biofuels 2019 12:18

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  34. Content type: Research

    Methanol has attracted increased attention as a non-food alternative carbon source to sugar for biological production of chemicals and fuels. Moreover, the high degree of reduction of methanol offers some adva...

    Authors: Xin Wang, Xuelin Wang, Xiaolu Lu, Chen Ma, Kequan Chen and Pingkai Ouyang

    Citation: Biotechnology for Biofuels 2019 12:17

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  35. Content type: Research

    Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications. Hiptage benghalensis seed oil contains a high amount of ricinoleic acid (~ 80%) and represents an emerging source of this unu...

    Authors: Bo Tian, Tianquan Lu, Yang Xu, Ruling Wang and Guanqun Chen

    Citation: Biotechnology for Biofuels 2019 12:16

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  36. Content type: Research

    The recalcitrance of cellulosic biomass is widely recognized as a key barrier to cost-effective biological processing to fuels and chemicals, but the relative impacts of physical, chemical and genetic interven...

    Authors: Evert K. Holwerda, Robert S. Worthen, Ninad Kothari, Ronald C. Lasky, Brian H. Davison, Chunxiang Fu, Zeng-Yu Wang, Richard A. Dixon, Ajaya K. Biswal, Debra Mohnen, Richard S. Nelson, Holly L. Baxter, Mitra Mazarei, C. Neal Stewart Jr., Wellington Muchero, Gerald A. Tuskan…

    Citation: Biotechnology for Biofuels 2019 12:15

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    The Correction to this article has been published in Biotechnology for Biofuels 2019 12:25

  37. Content type: Research

    In the past few decades, microalgae biofuel has become one of the most interesting sources of renewable energy. However, the higher cost of microalgae biofuel compared to that of petroleum prevented microalgae...

    Authors: Nannan Li, Yan Zhang, Hongjun Meng, Shengting Li, Shufeng Wang, Zhongchun Xiao, Peng Chang, Xiaohui Zhang, Qing Li, Liang Guo, Yasuo Igarashi and Feng Luo

    Citation: Biotechnology for Biofuels 2019 12:14

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  38. Content type: Research

    The alginate oligosaccharides have been widely used in agriculture, medicine, and food industries due to their versatile physiological functions such as antioxidant, anticoagulant, and antineoplastic activitie...

    Authors: Benwei Zhu, Fang Ni, Yun Sun, Limin Ning and Zhong Yao

    Citation: Biotechnology for Biofuels 2019 12:13

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  39. Content type: Research

    Due to biomass recalcitrance, including complexity of lignocellulosic matrix, crystallinity of cellulose, and inhibition of lignin, the bioconversion of lignocellulosic biomass is difficult and inefficient. Th...

    Authors: Yanjuan Zhang, Min Huang, Jianmei Su, Huayu Hu, Mei Yang, Zuqiang Huang, Dong Chen, Juan Wu and Zhenfei Feng

    Citation: Biotechnology for Biofuels 2019 12:12

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  40. Content type: Research

    Genetic modification of plant cell walls has been implemented to reduce lignocellulosic recalcitrance for biofuel production. Plant glycoside hydrolase family 9 (GH9) comprises endo-β-1,4-glucanase in plants. ...

    Authors: Jiangfeng Huang, Tao Xia, Guanhua Li, Xianliang Li, Ying Li, Yanting Wang, Youmei Wang, Yuanyuan Chen, Guosheng Xie, Feng-Wu Bai, Liangcai Peng and Lingqiang Wang

    Citation: Biotechnology for Biofuels 2019 12:11

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  41. Content type: Research

    Cellulosic biofuels are sustainable compared to fossil fuels. However, inhibitors, such as acetic acid generated during lignocellulose pretreatment and hydrolysis, would significantly inhibit microbial ferment...

    Authors: Bo Wu, Han Qin, Yiwei Yang, Guowei Duan, Shihui Yang, Fengxue Xin, Chunyan Zhao, Huanhuan Shao, Yanwei Wang, Qili Zhu, Furong Tan, Guoquan Hu and Mingxiong He

    Citation: Biotechnology for Biofuels 2019 12:10

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  42. Content type: Research

    Cyanobacteria are potential sources for third generation biofuels. Their capacity for biofuel production has been widely improved using metabolically engineered strains. In this study, we employed metabolic en...

    Authors: Kamonchanock Eungrasamee, Rui Miao, Aran Incharoensakdi, Peter Lindblad and Saowarath Jantaro

    Citation: Biotechnology for Biofuels 2019 12:8

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  43. Content type: Research

    Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi rem...

    Authors: Mei-Yuan Zhang, Shuai Zhao, Yuan-Ni Ning, Li-Hao Fu, Cheng-Xi Li, Qi Wang, Ran You, Chen-Ying Wang, Han-Nan Xu, Xue-Mei Luo and Jia-Xun Feng

    Citation: Biotechnology for Biofuels 2019 12:7

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  44. Content type: Research

    Based on our previous studies of 17 Prunus sibirica germplasms, one plus tree with high quality and quantity of seed oils has emerged as novel potential source of biodiesel. To better develop P. sibirica seed oil...

    Authors: Jia Wang, Weijun Lin, Zhongdong Yin, Libing Wang, ShuBin Dong, Jiyong An, Zixin Lin, Haiyan Yu, Lingling Shi, Shanzhi Lin and Shaoliang Chen

    Citation: Biotechnology for Biofuels 2019 12:6

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  45. Content type: Research

    With the increasing global population and increasing demand for food, the generation of food waste and animal manure increases. Anaerobic digestion is one of the best available technologies for food waste and ...

    Authors: Yan Jiang, Conor Dennehy, Peadar G. Lawlor, Zhenhu Hu, Matthew McCabe, Paul Cormican, Xinmin Zhan and Gillian E. Gardiner

    Citation: Biotechnology for Biofuels 2019 12:5

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  46. Content type: Research

    As a ubiquitous filamentous fungal, Aspergillus spp. play a critical role in lignocellulose degradation, which was also defined as considerable cell factories for organic acids and industrially relevant enzymes p...

    Authors: Jiaxi Miao, Mengmeng Wang, Lei Ma, Tuo Li, Qiwei Huang, Dongyang Liu and Qirong Shen

    Citation: Biotechnology for Biofuels 2019 12:4

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  47. Content type: Research

    To improve process economics for production of fuels and chemicals from lignocellulosic biomass, high solids concentrations are applied in enzymatic hydrolysis, to increase product concentration and reduce ene...

    Authors: Noah D. Weiss, Claus Felby and Lisbeth G. Thygesen

    Citation: Biotechnology for Biofuels 2019 12:3

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  48. Content type: Research

    Harnessing the halotolerant characteristics of microalgae provides a viable alternative for sustainable biomass and triacylglyceride (TAG) production. Scenedesmus sp. IITRIND2 is a fast growing fresh water microa...

    Authors: Neha Arora, Poonam Kumari, Amit Kumar, Rashmi Gangwar, Khushboo Gulati, Parul A. Pruthi, Ramasare Prasad, Dinesh Kumar, Vikas Pruthi and Krishna Mohan Poluri

    Citation: Biotechnology for Biofuels 2019 12:2

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  49. Content type: Research

    The forest biorefinery plays an important part in the evolving circular bioeconomy due to its capacity to produce a portfolio of bio-based and sustainable fuels, chemicals, and materials. To tap into its true ...

    Authors: Johanna Olsson, Vera Novy, Fredrik Nielsen, Ola Wallberg and Mats Galbe

    Citation: Biotechnology for Biofuels 2019 12:1

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