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  1. The secondary alcohol 2-butanol has many important applications, e.g., as a solvent. Industrially, it is usually made by sulfuric acid-catalyzed hydration of butenes. Microbial production of 2-butanol has also...

    Authors: Mette J. Mar, Joakim M. Andersen, Vijayalakshmi Kandasamy, Jianming Liu, Christian Solem and Peter R. Jensen

    Citation: Biotechnology for Biofuels 2020 13:45

    Content type: Research

    Published on:

  2. For bioethanol production from lignocellulosic biomass, phenolics derived from pretreatment have been generally considered as highly inhibitory towards enzymatic hydrolysis and fermentation. As phenolics are p...

    Authors: Xiangxue Chen, Rui Zhai, Ying Li, Xinchuan Yuan, Zhi-Hua Liu and Mingjie Jin

    Citation: Biotechnology for Biofuels 2020 13:44

    Content type: Research

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  3. Biological conversion of lignocellulosic biomass is significantly hindered by feedstock recalcitrance, which is typically assessed through an enzymatic digestion assay, often preceded by a thermal and/or chemi...

    Authors: Christopher T. Straub, Ryan G. Bing, Jack P. Wang, Vincent L. Chiang, Michael W. W. Adams and Robert M. Kelly

    Citation: Biotechnology for Biofuels 2020 13:43

    Content type: Research

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  4. Brassica napus provides approximately 13–16% of global vegetable oil for human consumption and biodiesel production. Plant height (PH) is a key trait that affects plant architecture, seed yield and harvest index....

    Authors: Xiaodong Wang, Ming Zheng, Hongfang Liu, Liang Zhang, Feng Chen, Wei Zhang, Shihang Fan, Menlu Peng, Maolong Hu, Hanzhong Wang, Jiefu Zhang and Wei Hua

    Citation: Biotechnology for Biofuels 2020 13:42

    Content type: Research

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  5. 5-Aminolevulinic acid (5-ALA) is a promising biostimulant, feed nutrient, and photodynamic drug with wide applications in modern agriculture and therapy. Considering the complexity and low yield of chemical sy...

    Authors: Jiuzhou Chen, Yu Wang, Xuan Guo, Deming Rao, Wenjuan Zhou, Ping Zheng, Jibin Sun and Yanhe Ma

    Citation: Biotechnology for Biofuels 2020 13:41

    Content type: Research

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  6. Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been variably successful. Here, we aim to understand this variation by a multif...

    Authors: Evert K. Holwerda, Daniel G. Olson, Natalie M. Ruppertsberger, David M. Stevenson, Sean J. L. Murphy, Marybeth I. Maloney, Anthony A. Lanahan, Daniel Amador-Noguez and Lee R. Lynd

    Citation: Biotechnology for Biofuels 2020 13:40

    Content type: Research

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  7. The global energy crisis and limited supply of petroleum fuels have rekindled the interest in utilizing a sustainable biomass to produce biofuel. Butanol, an advanced biofuel, is a superior renewable resource ...

    Authors: Shubo Li, Li Huang, Chengzhu Ke, Zongwen Pang and Liming Liu

    Citation: Biotechnology for Biofuels 2020 13:39

    Content type: Review

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  8. The necessity to develop high lipid-producing microalgae is emphasized for the commercialization of microalgal biomass, which is environmentally friendly and sustainable. Nannochloropsis are one of the best indus...

    Authors: Ae Jin Ryu, Nam Kyu Kang, Seungjib Jeon, Dong Hoon Hur, Eun Mi Lee, Do Yup Lee, Byeong-ryool Jeong, Yong Keun Chang and Ki Jun Jeong

    Citation: Biotechnology for Biofuels 2020 13:46

    Content type: Research

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  9. Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent redox enzymes that cleave recalcitrant biopolymers such as cellulose, chitin, starch and hemicelluloses. Although LPMOs receive ample interest i...

    Authors: Frantisek Filandr, Petr Man, Petr Halada, Hucheng Chang, Roland Ludwig and Daniel Kracher

    Citation: Biotechnology for Biofuels 2020 13:37

    Content type: Research

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  10. Microbial fuel cell (MFC) convokes microorganism to convert biomass into electricity. However, most well-known electrogenic strains cannot directly use glucose to produce valuable products. Zymomonas mobilis, a p...

    Authors: Bo-Yu Geng, Lian-Ying Cao, Feng Li, Hao Song, Chen-Guang Liu, Xin-Qing Zhao and Feng-Wu Bai

    Citation: Biotechnology for Biofuels 2020 13:36

    Content type: Research

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  11. Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment ...

    Authors: Kiruthika Thangavelu, Pugalendhi Sundararaju, Naganandhini Srinivasan, Iniyakumar Muniraj and Sivakumar Uthandi

    Citation: Biotechnology for Biofuels 2020 13:35

    Content type: Research

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  12. Bamboo, a lignocellulosic feedstock, is considered as a potentially excellent raw material and evaluated for lignocellulose degradation and bioethanol production, with a focus on using physical and chemical pr...

    Authors: Yuanqiu Li, Lu Lei, Li Zheng, Ximeng Xiao, Hao Tang and Chaobing Luo

    Citation: Biotechnology for Biofuels 2020 13:34

    Content type: Research

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  13. Pseudomonas putida is a promising candidate for the industrial production of biofuels and biochemicals because of its high tolerance to toxic compounds and its ability to grow on a wide variety of substrates. Eng...

    Authors: Milenko Tokic, Vassily Hatzimanikatis and Ljubisa Miskovic

    Citation: Biotechnology for Biofuels 2020 13:33

    Content type: Research

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  14. Endoglucanase has been extensively employed in industrial processes as a key biocatalyst for lignocellulosic biomass degradation. Thermostable endoglucanases with high catalytic activity at elevated temperatur...

    Authors: Chao Han, Qunqing Wang, Yanxu Sun, Ruirui Yang, Mengyu Liu, Siqi Wang, Yifan Liu, Lifan Zhou and Duochuan Li

    Citation: Biotechnology for Biofuels 2020 13:30

    Content type: Research

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  15. Protein-based bioconversion has been demonstrated as a sustainable approach to produce higher alcohols and ammonia fertilizers. However, owing to the switchover from transcription mediated by the bacterial RNA...

    Authors: Lianjie Ma, Liwei Guo, Yunpeng Yang, Kai Guo, Yajun Yan, Xiaoyan Ma and Yi-Xin Huo

    Citation: Biotechnology for Biofuels 2020 13:29

    Content type: Research

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  16. Pretreatment of lignocellulosic biomass generates different types of inhibitors (e.g., furfural and acetic acid), which could remarkably inhibit subsequent ethanol fermentation. Here, biochar as an additive in...

    Authors: Wei-ting Wang, Li-chun Dai, Bo Wu, Bu-fan Qi, Tian-fang Huang, Guo-quan Hu and Ming-xiong He

    Citation: Biotechnology for Biofuels 2020 13:28

    Content type: Research

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  17. The fungus Aspergillus niger is an important industrial organism for citric acid fermentation; one of the most efficient biotechnological processes. Previously we introduced a dynamic model that captures this pro...

    Authors: Daniel J. Upton, Simon J. McQueen-Mason and A. Jamie Wood

    Citation: Biotechnology for Biofuels 2020 13:27

    Content type: Research

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  18. Fungal decomposition of wood is considered as a strictly aerobic process. However, recent findings on wood-decaying fungi to produce ethanol from various lignocelluloses under oxygen-depleted conditions lead u...

    Authors: Hans Kristian Mattila, Mari Mäkinen and Taina Lundell

    Citation: Biotechnology for Biofuels 2020 13:26

    Content type: Research

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  19. Microorganisms in biogas reactors are essential for degradation of organic matter and methane production. However, a comprehensive genome-centric comparison, including relevant metadata for each sample, is sti...

    Authors: Stefano Campanaro, Laura Treu, Luis M. Rodriguez-R, Adam Kovalovszki, Ryan M. Ziels, Irena Maus, Xinyu Zhu, Panagiotis G. Kougias, Arianna Basile, Gang Luo, Andreas Schlüter, Konstantinos T. Konstantinidis and Irini Angelidaki

    Citation: Biotechnology for Biofuels 2020 13:25

    Content type: Research

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  20. Production and use of bio-based products offer advantages over conventional petrochemicals, yet the relatively high cost of production has restricted their mainstream adoption. Optimization of wastewater treat...

    Authors: Tyler Tobin, Rick Gustafson, Renata Bura and Heidi L. Gough

    Citation: Biotechnology for Biofuels 2020 13:24

    Content type: Research

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  21. Cellulosic biomass is a promising resource for bioethanol production. However, various sugars in plant biomass hydrolysates including cellodextrins, cellobiose, glucose, xylose, and arabinose, are poorly ferme...

    Authors: Jinyang Li, Yongli Zhang, Jingen Li, Tao Sun and Chaoguang Tian

    Citation: Biotechnology for Biofuels 2020 13:23

    Content type: Research

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  22. The four-carbon dicarboxylic acids of the tricarboxylic acid cycle (malate, fumarate and succinate) remain promising bio-based alternatives to various precursor chemicals derived from fossil-based feed stocks....

    Authors: Reuben M. Swart, Francois le Roux, Andre Naude, Nicolaas W. de Jongh and Willie Nicol

    Citation: Biotechnology for Biofuels 2020 13:22

    Content type: Research

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  23. Biofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophili...

    Authors: Eun Jae Kim, Sanghee Kim, Han-Gu Choi and Se Jong Han

    Citation: Biotechnology for Biofuels 2020 13:20

    Content type: Research

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  24. Cellulase adsorbed on cellulose is productive and helpful to produce reducing sugars in enzymatic hydrolysis of lignocellulose; however, cellulase adsorbed on lignin is non-productive. Increasing productive ad...

    Authors: Wenqiu Zheng, Tianqing Lan, Hui Li, Guojun Yue and Haifeng Zhou

    Citation: Biotechnology for Biofuels 2020 13:19

    Content type: Research

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  25. Vanillin is the main byproduct of alkaline-pretreated lignocellulosic biomass during the process of fermentable-sugar production and a potent inhibitor of ethanol production by yeast. Yeast cells are usually e...

    Authors: Shou Ito, Kiyota Sakai, Vladislav Gamaleev, Masafumi Ito, Masaru Hori, Masashi Kato and Motoyuki Shimizu

    Citation: Biotechnology for Biofuels 2020 13:18

    Content type: Research

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  26. Synechococcus sp. PCC 7002 is an attractive organism as a feedstock and for photoautotrophic production of biofuels and biochemicals due to its fast growth and ability to grow in marine/brackish medium. Previous ...

    Authors: Jai Kumar Gupta, Preeti Rai, Kavish Kumar Jain and Shireesh Srivastava

    Citation: Biotechnology for Biofuels 2020 13:17

    Content type: Research

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  27. Cyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules.

    Authors: Claudia Durall, Pia Lindberg, Jianping Yu and Peter Lindblad

    Citation: Biotechnology for Biofuels 2020 13:16

    Content type: Research

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  28. Biofuels and value-added biochemicals derived from renewable biomass via biochemical conversion have attracted considerable attention to meet global sustainable energy and environmental goals. Isobutanol is a ...

    Authors: Mengyue Qiu, Wei Shen, Xiongyin Yan, Qiaoning He, Dongbo Cai, Shouwen Chen, Hui Wei, Eric P. Knoshaug, Min Zhang, Michael E. Himmel and Shihui Yang

    Citation: Biotechnology for Biofuels 2020 13:15

    Content type: Research

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  29. Lignocellulosic biomass has been commonly regarded as a potential feedstock for the production of biofuels and biochemicals. High sugar yields and the complete bioconversion of all the lignocellulosic sugars i...

    Authors: Zhiwei Gong, Xuemin Wang, Wei Yuan, Yanan Wang, Wenting Zhou, Guanghui Wang and Yi Liu

    Citation: Biotechnology for Biofuels 2020 13:13

    Content type: Research

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  30. Lignocellulosic biorefinery offers economical and sustainable production of fuels and chemicals. Saccharomyces cerevisiae, a promising industrial host for biorefinery, has been intensively developed to expand its...

    Authors: Phuong Hoang Nguyen Tran, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um and Sun-Mi Lee

    Citation: Biotechnology for Biofuels 2020 13:12

    Content type: Research

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  31. Molecular-scale mechanisms of the enzymatic breakdown of cellulosic biomass into fermentable sugars are still poorly understood, with a need for independent measurements of enzyme kinetic parameters. We measur...

    Authors: Akshata R. Mudinoor, Peter M. Goodwin, Raghavendra U. Rao, Nardrapee Karuna, Alex Hitomi, Jennifer Nill and Tina Jeoh

    Citation: Biotechnology for Biofuels 2020 13:10

    Content type: Research

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  32. As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically c...

    Authors: Chunfen Fan, Hua Yu, Shifei Qin, Yongli Li, Aftab Alam, Changzhen Xu, Di Fan, Qingwei Zhang, Yanting Wang, Wanbin Zhu, Liangcai Peng and Keming Luo

    Citation: Biotechnology for Biofuels 2020 13:9

    Content type: Research

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  33. As a renewable carbon source, biomass energy not only helps in resolving the management problems of lignocellulosic wastes, but also helps to alleviate the global climate change by controlling environmental po...

    Authors: Xiaoli Li, Junjing Sha, Yihua Xia, Kuichuan Sheng, Yufei Liu and Yong He

    Citation: Biotechnology for Biofuels 2020 13:8

    Content type: Research

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  34. Filamentous fungi have the ability to efficiently decompose plant biomass, and thus are widely used in the biofuel and bioprocess industries. In process, ambient pH has been reported to strongly affect the per...

    Authors: Youzhi Miao, Xing Chen, Tuo Li, Han Zhu, Siyu Tang, Dongyang Liu and Qirong Shen

    Citation: Biotechnology for Biofuels 2020 13:6

    Content type: Research

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  35. Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isom...

    Authors: Misun Lee, Henriëtte J. Rozeboom, Eline Keuning, Paul de Waal and Dick B. Janssen

    Citation: Biotechnology for Biofuels 2020 13:5

    Content type: Research

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  36. Cultivation of microalgae in wastewater could significantly contribute to wastewater treatment, biodiesel production, and thus the transition to renewable energy. However, more information on effects of enviro...

    Authors: Jean Claude Nzayisenga, Xavier Farge, Sophia Leticia Groll and Anita Sellstedt

    Citation: Biotechnology for Biofuels 2020 13:4

    Content type: Research

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  37. Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) V...

    Authors: Ruiling Gao, Zifu Li, Xiaoqin Zhou, Wenjun Bao, Shikun Cheng and Lei Zheng

    Citation: Biotechnology for Biofuels 2020 13:3

    Content type: Research

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  38. Lignocellulosic biomass, which is composed of cellulose, hemicellulose and lignin, represents the most abundant renewable carbon source with significant potential for the production of sustainable chemicals an...

    Authors: Xue Chen, Kaili Zhang, Ling-Ping Xiao, Run-Cang Sun and Guoyong Song

    Citation: Biotechnology for Biofuels 2020 13:2

    Content type: Research

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  39. The critical issue in the competitiveness between bioengineering and chemical engineering is the products titer and the volume productivity. The most direct and effective approach usually employs high-density ...

    Authors: Xia Hua, Xin Zhou, GenLai Du and Yong Xu

    Citation: Biotechnology for Biofuels 2020 13:1

    Content type: Research

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  40. Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in...

    Authors: Yide Huang, Yafei Zhang, Suhuan Li, Ting Lin, Jingwen Wu and Yao Lin

    Citation: Biotechnology for Biofuels 2019 12:300

    Content type: Research

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  41. Enzymatic hydrolysis continues to have a significant projected production cost for the biological conversion of biomass to fuels and chemicals, motivating research into improved enzyme and reactor technologies...

    Authors: James J. Lischeske and Jonathan J. Stickel

    Citation: Biotechnology for Biofuels 2019 12:299

    Content type: Research

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  42. Programmed cell death (PCD) induced by acetic acid, the main by-product released during cellulosic hydrolysis, cast a cloud over lignocellulosic biofuel fermented by Saccharomyces cerevisiae and became a burning ...

    Authors: Jingjin Hu, Yachen Dong, Wei Wang, Wei Zhang, Hanghang Lou and Qihe Chen

    Citation: Biotechnology for Biofuels 2019 12:298

    Content type: Research

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  43. The bioconversion of lignocellulose to fermentable C5/C6-saccharides is composed of pretreatment and enzymatic hydrolysis. Lignin, as one of the main components, resists lignocellulose to be bio-digested. Alka...

    Authors: Xianqin Lu, Can Li, Shengkui Zhang, Xiaohan Wang, Wenqing Zhang, Shouguo Wang and Tao Xia

    Citation: Biotechnology for Biofuels 2019 12:297

    Content type: Research

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  44. Efficient conversion of plant biomass to commodity chemicals is an important challenge that needs to be solved to enable a sustainable bioeconomy. Deconstruction of biomass to sugars and lignin yields a wide v...

    Authors: George L. Peabody V, Joshua R. Elmore, Jessica Martinez-Baird and Adam M. Guss

    Citation: Biotechnology for Biofuels 2019 12:295

    Content type: Research

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