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  1. We and other workers have shown that accessory enzymes, such as β-glucosidase, xylanase, and cellulase cofactors, such as GH61, can considerably enhance the hydrolysis effectiveness of cellulase cocktails when...

    Authors: Jinguang Hu, Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2011 4:36

    Content type: Research

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  2. Ethanol production from paper sludge (PS) by simultaneous saccharification and fermentation (SSF) is considered to be the most appropriate way to process PS, as it contains negligible lignin. In this study, SS...

    Authors: Joni Prasetyo, Kazuya Naruse, Tatsuya Kato, Chuenchit Boonchird, Satoshi Harashima and Enoch Y Park

    Citation: Biotechnology for Biofuels 2011 4:35

    Content type: Research

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  3. Microalgae are a promising feedstock for biofuel and bioenergy production due to their high photosynthetic efficiencies, high growth rates and no need for external organic carbon supply. In this study, utiliza...

    Authors: Aino-Maija Lakaniemi, Christopher J Hulatt, David N Thomas, Olli H Tuovinen and Jaakko A Puhakka

    Citation: Biotechnology for Biofuels 2011 4:34

    Content type: Research

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  4. In order to generate biofuels, insoluble cellulosic substrates are pretreated andsubsequently hydrolyzed with cellulases. One way to pretreat cellulose in a safeand environmentally friendly manner is to apply,...

    Authors: Gernot Jäger, Michele Girfoglio, Florian Dollo, Roberto Rinaldi, Hans Bongard, Ulrich Commandeur, Rainer Fischer, Antje C Spiess and Jochen Büchs

    Citation: Biotechnology for Biofuels 2011 4:33

    Content type: Research

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  5. Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The ...

    Authors: Mattias Ljunggren, Karin Willquist, Guido Zacchi and Ed WJ van Niel

    Citation: Biotechnology for Biofuels 2011 4:31

    Content type: Research

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  6. The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrol...

    Authors: Marja Ilmén, Riaan den Haan, Elena Brevnova, John McBride, Erin Wiswall, Allan Froehlich, Anu Koivula, Sanni P Voutilainen, Matti Siika-aho, Daniël C la Grange, Naomi Thorngren, Simon Ahlgren, Mark Mellon, Kristen Deleault, Vineet Rajgarhia, Willem H van Zyl…

    Citation: Biotechnology for Biofuels 2011 4:30

    Content type: Research

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  7. Cellulases and related hydrolytic enzymes represent a key cost factor for biochemical conversion of cellulosic biomass feedstocks to sugars for biofuels and chemicals production. The US Department of Energy (D...

    Authors: James D McMillan, Edward W Jennings, Ali Mohagheghi and Mildred Zuccarello

    Citation: Biotechnology for Biofuels 2011 4:29

    Content type: Research

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  8. Atmospheric pressure chemical ionisation mass spectrometry (APCI-MS) offers advantages as a rapid analytical technique for the quantification of three biomass degradation products (acetic acid, formic acid and...

    Authors: Scott M Davies, Rob S Linforth, Stuart J Wilkinson, Katherine A Smart and David J Cook

    Citation: Biotechnology for Biofuels 2011 4:28

    Content type: Methodology

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  9. The trichothecene mycotoxin deoxynivalenol (DON) may be concentrated in distillers dried grains with solubles (DDGS; a co-product of fuel ethanol fermentation) when grain containing DON is used to produce fuel...

    Authors: Piyum A Khatibi, Justin Montanti, Nhuan P Nghiem, Kevin B Hicks, Greg Berger, Wynse S Brooks, Carl A Griffey and David G Schmale III

    Citation: Biotechnology for Biofuels 2011 4:26

    Content type: Research

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  10. Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identifi...

    Authors: Cuimin Hu, Siguo Wu, Qian Wang, Guojie Jin, Hongwei Shen and Zongbao K Zhao

    Citation: Biotechnology for Biofuels 2011 4:25

    Content type: Research

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  11. Neocallimastix patriciarum is one of the common anaerobic fungi in the digestive tracts of ruminants that can actively digest cellulosic materials, and its cellulases have great potential for hydrolyzing cellulos...

    Authors: Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou…

    Citation: Biotechnology for Biofuels 2011 4:24

    Content type: Research

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  12. To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenom...

    Authors: Luen-Luen Li, Safiyh Taghavi, Sean M McCorkle, Yian-Biao Zhang, Michael G Blewitt, Roman Brunecky, William S Adney, Michael E Himmel, Phillip Brumm, Colleen Drinkwater, David A Mead, Susannah G Tringe and Daniel van der Lelie

    Citation: Biotechnology for Biofuels 2011 4:23

    Content type: Research

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  13. As the supply of starch grain and sugar cane, currently the main feedstocks for bioethanol production, become limited, lignocelluloses will be sought as alternative materials for bioethanol production. Product...

    Authors: Yong Tang, Danqing Zhao, Carrasco Cristhian and Jianxin Jiang

    Citation: Biotechnology for Biofuels 2011 4:22

    Content type: Research

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  14. Isobutanol can be a better biofuel than ethanol due to its higher energy density and lower hygroscopicity. Furthermore, the branched-chain structure of isobutanol gives a higher octane number than the isomeric n-...

    Authors: Xiao Chen, Kristian F Nielsen, Irina Borodina, Morten C Kielland-Brandt and Kaisa Karhumaa

    Citation: Biotechnology for Biofuels 2011 4:21

    Content type: Research

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  15. The use of energy crops and agricultural residues is expected to increase to fulfil the legislative demands of bio-based components in transport fuels. Ensiling methods, adapted from the feed sector, are suita...

    Authors: Annukka Pakarinen, Pekka Maijala, Seija Jaakkola, Frederick L Stoddard, Maritta Kymäläinen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:20

    Content type: Research

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  16. The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids betwe...

    Authors: Michael H Studer, Simone Brethauer, Jaclyn D DeMartini, Heather L McKenzie and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:19

    Content type: Research

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  17. Hemicellulose is often credited with being one of the important physical barriers to enzymatic hydrolysis of cellulose, and acts by blocking enzyme access to the cellulose surface. In addition, our recent rese...

    Authors: Qing Qing and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:18

    Content type: Research

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  18. Termites are highly effective at degrading lignocelluloses, and thus can be used as a model for studying plant cell-wall degradation in biological systems. However, the process of lignin deconstruction and/or ...

    Authors: Jing Ke, Dhrubojyoti D Laskar, Deepak Singh and Shulin Chen

    Citation: Biotechnology for Biofuels 2011 4:17

    Content type: Research

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  19. Pretreatment is a critical step in the conversion of lignocellulose to fermentable sugars. Although many pretreatment processes are currently under investigation, none of them are entirely satisfactory in rega...

    Authors: Goutami Banerjee, Suzana Car, John S Scott-Craig, David B Hodge and Jonathan D Walton

    Citation: Biotechnology for Biofuels 2011 4:16

    Content type: Research

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  20. The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi ha...

    Authors: Chunjie Xia, Jianguo Zhang, Weidong Zhang and Bo Hu

    Citation: Biotechnology for Biofuels 2011 4:15

    Content type: Research

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  21. Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment ...

    Authors: Stefan Kühnel, Henk A Schols and Harry Gruppen

    Citation: Biotechnology for Biofuels 2011 4:14

    Content type: Research

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  22. Short rotation coppice willow is a potential lignocellulosic feedstock in the United Kingdom and elsewhere; however, research on optimising willow specifically for bioethanol production has started developing ...

    Authors: Nicholas JB Brereton, Frederic E Pitre, Michael J Ray, Angela Karp and Richard J Murphy

    Citation: Biotechnology for Biofuels 2011 4:13

    Content type: Research

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  23. In the hydrolysis of lignocellulosic materials, thermostable enzymes decrease the amount of enzyme needed due to higher specific activity and elongate the hydrolysis time due to improved stability. For cost-ef...

    Authors: Junhua Zhang, Matti Siika-aho, Terhi Puranen, Ming Tang, Maija Tenkanen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:12

    Content type: Research

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  24. The recent development of improved enzymes and pentose-using yeast for cellulosic ethanol processes calls for new attention to the lignocellulose pretreatment step. This study assessed the influence of pretrea...

    Authors: Mads Pedersen, Katja S Johansen and Anne S Meyer

    Citation: Biotechnology for Biofuels 2011 4:11

    Content type: Research

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  25. When scaling up lignocellulose-based ethanol production, the desire to increase the final ethanol titer after fermentation can introduce problems. A high concentration of water-insoluble solids (WIS) is needed...

    Authors: Benny Palmqvist, Magnus Wiman and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2011 4:10

    Content type: Research

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  26. Xylose isomerase (XI) catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed in Saccharomyces cerevisiae, the m...

    Authors: Nádia Skorupa Parachin and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2011 4:9

    Content type: Research

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  27. Hydrolysis of cellulose requires the action of the cellulolytic enzymes endoglucanase, cellobiohydrolase and β-glucosidase. The expression ratios and synergetic effects of these enzymes significantly influence...

    Authors: Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda and Akihiko Kondo

    Citation: Biotechnology for Biofuels 2011 4:8

    Content type: Research

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  28. Lignin and hemicelluloses are the major components limiting enzyme infiltration into cell walls. Determination of the topochemical distribution of lignin and aromatics in sugar cane might provide important dat...

    Authors: Germano Siqueira, Adriane MF Milagres, Walter Carvalho, Gerald Koch and André Ferraz

    Citation: Biotechnology for Biofuels 2011 4:7

    Content type: Research

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  29. Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, ...

    Authors: Yonghong Meng, Guili Wang, Na Yang, Zhiqi Zhou, Yuejuan Li, Xiaomei Liang, Jinnan Chen, Ying Li and Jilun Li

    Citation: Biotechnology for Biofuels 2011 4:6

    Content type: Research

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  30. High enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretre...

    Authors: Dahai Gao, Nirmal Uppugundla, Shishir PS Chundawat, Xiurong Yu, Spencer Hermanson, Krishne Gowda, Phillip Brumm, David Mead, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2011 4:5

    Content type: Research

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  31. The discovery and development of novel plant cell wall degrading enzymes is a key step towards more efficient depolymerization of polysaccharides to fermentable sugars for the production of liquid transportati...

    Authors: Brian C King, Katrina D Waxman, Nicholas V Nenni, Larry P Walker, Gary C Bergstrom and Donna M Gibson

    Citation: Biotechnology for Biofuels 2011 4:4

    Content type: Research

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  32. A range of lignocellulosic feedstocks (including agricultural, softwood and hardwood substrates) were pretreated with either sulfur dioxide-catalyzed steam or an ethanol organosolv procedure to try to establis...

    Authors: Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2011 4:3

    Content type: Research

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  33. Thermostable enzymes have several benefits in lignocellulose processing. In particular, they potentially allow the use of increased substrate concentrations (because the substrate viscosity decreases as the te...

    Authors: Nóra Szijártó, Emma Horan, Junhua Zhang, Terhi Puranen, Matti Siika-aho and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:2

    Content type: Research

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  34. The glycoside hydrolase family 5 endocellulase, E1 (Cel5A), from Acidothermus cellulolyticus was transformed into both Nicotiana tabacum and Zea mays with expression targeted to the cell wall under a constitutive...

    Authors: Roman Brunecky, Michael J Selig, Todd B Vinzant, Michael E Himmel, David Lee, Michael J Blaylock and Stephen R Decker

    Citation: Biotechnology for Biofuels 2011 4:1

    Content type: Research

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  35. Lignin is embedded in the plant cell wall matrix, and impedes the enzymatic saccharification of lignocellulosic feedstocks. To investigate whether enzymatic digestibility of cell wall materials can be improved...

    Authors: Xu Li, Eduardo Ximenes, Youngmi Kim, Mary Slininger, Richard Meilan, Michael Ladisch and Clint Chapple

    Citation: Biotechnology for Biofuels 2010 3:27

    Content type: Research

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  36. Degradation of the toxic compounds generated in the harsh pretreatment of lignocellulose is an inevitable step in reducing the toxin level for conducting practical enzymatic hydrolysis and ethanol fermentation...

    Authors: Jian Zhang, Zhinan Zhu, Xiaofeng Wang, Nan Wang, Wei Wang and Jie Bao

    Citation: Biotechnology for Biofuels 2010 3:26

    Content type: Research

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  37. Variations in sugar yield due to genotypic qualities of feedstock are largely undescribed for pilot-scale ethanol processing. Our objectives were to compare glucose and xylose yield (conversion and total sugar...

    Authors: Jane Lindedam, Sander Bruun, Henning Jørgensen, Claus Felby and Jakob Magid

    Citation: Biotechnology for Biofuels 2010 3:25

    Content type: Research

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  38. Cell wall resistance represents the main barrier for the production of second generation biofuels. The deconstruction of lignocellulose can provide sugars for the production of fuels or other industrial produc...

    Authors: Leonardo D Gomez, Caragh Whitehead, Abdellah Barakate, Claire Halpin and Simon J McQueen-Mason

    Citation: Biotechnology for Biofuels 2010 3:23

    Content type: Research

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  39. Enzymes for plant cell wall deconstruction are a major cost in the production of ethanol from lignocellulosic biomass. The goal of this research was to develop optimized synthetic mixtures of enzymes for multi...

    Authors: Goutami Banerjee, Suzana Car, John S Scott-Craig, Melissa S Borrusch and Jonathan D Walton

    Citation: Biotechnology for Biofuels 2010 3:22

    Content type: Research

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  40. The complex technology of converting lignocellulose to fuels such as ethanol has advanced rapidly over the past few years, and enzymes are a critical component of this technology. The production of effective e...

    Authors: Suzanne E Lantz, Frits Goedegebuur, Ronald Hommes, Thijs Kaper, Bradley R Kelemen, Colin Mitchinson, Louise Wallace, Jerry Ståhlberg and Edmundo A Larenas

    Citation: Biotechnology for Biofuels 2010 3:20

    Content type: Research

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  41. Overexpression of the PGM2 gene encoding phosphoglucomutase (Pgm2p) has been shown to improve galactose utilization both under aerobic and under anaerobic conditions. Similarly, xylose utilization has been improv...

    Authors: Rosa Garcia Sanchez, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2010 3:19

    Content type: Research

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  42. It is important to generate biofuels and society must be weaned from its dependency on fossil fuels. In order to produce biofuels, lignocellulose is pretreated and the resulting cellulose is hydrolyzed by cell...

    Authors: Gernot Jäger, Zhuojun Wu, Kerstin Garschhammer, Philip Engel, Tobias Klement, Roberto Rinaldi, Antje C Spiess and Jochen Büchs

    Citation: Biotechnology for Biofuels 2010 3:18

    Content type: Research

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  43. Simultaneous saccharification and co-fermentation (SSCF) has been recognized as a feasible option for ethanol production from xylose-rich lignocellulosic materials. To reach high ethanol concentration in the b...

    Authors: Kim Olofsson, Benny Palmqvist and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2010 3:17

    Content type: Research

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  44. Bioethanol can be produced from sugar-rich, starch-rich (first generation; 1G) or lignocellulosic (second generation; 2G) raw materials. Integration of 2G ethanol with 1G could facilitate the introduction of t...

    Authors: Borbála Erdei, Zsolt Barta, Bálint Sipos, Kati Réczey, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:16

    Content type: Research

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  45. The two-step dilute acid hydrolysis (DAH) of softwood is costly in energy demands and capital costs. However, it has the advantage that hydrolysis and subsequent removal of hemicellulose-derived sugars can be ...

    Authors: Peter Bösch, Ola Wallberg, Elisabeth Joelsson, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:15

    Content type: Research

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  46. To make lignocellulosic fuel ethanol economically competitive with fossil fuels, it is necessary to reduce the production cost. One way to achieve this is by increasing the substrate concentration in the produ...

    Authors: Kerstin Hoyer, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2010 3:14

    Content type: Research

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